Pseudodesulfovibrio aespoeensis (strain ATCC 700646 / DSM 10631 / Aspo-2) (Desulfovibrio aespoeensis)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Pseudodesulfovibrio; Pseudodesulfovibrio aespoeensis

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6VTH3|E6VTH3_PSEA9 Sigma-54 factor interaction domain-containing protein OS=Pseudodesulfovibrio aespoeensis (strain ATCC 700646 / DSM 10631 / Aspo-2) OX=643562 GN=Daes_1134 PE=4 SV=1
MM1 pKa = 7.16LTSLYY6 pKa = 10.65NLASSALKK14 pKa = 10.06NAQVSINNASNNIANADD31 pKa = 3.62TEE33 pKa = 4.93GYY35 pKa = 10.01QRR37 pKa = 11.84TEE39 pKa = 3.35AAYY42 pKa = 6.58EE43 pKa = 3.88TSYY46 pKa = 11.11SISIYY51 pKa = 10.08GLSVGTGANITSIISLKK68 pKa = 10.63DD69 pKa = 3.19QFVEE73 pKa = 4.21AQYY76 pKa = 11.61LDD78 pKa = 3.45ASADD82 pKa = 3.8LNRR85 pKa = 11.84EE86 pKa = 4.18SAALTYY92 pKa = 10.8LNQLDD97 pKa = 4.07SLLNQAEE104 pKa = 4.56GGLSEE109 pKa = 4.34TLTDD113 pKa = 6.78FFDD116 pKa = 3.24AWNALTTDD124 pKa = 4.23PDD126 pKa = 3.92SLSARR131 pKa = 11.84EE132 pKa = 4.17DD133 pKa = 3.49LLGIAEE139 pKa = 4.33TLVYY143 pKa = 10.66ALNSTADD150 pKa = 3.51GLEE153 pKa = 4.06TMEE156 pKa = 4.53AAINTEE162 pKa = 3.6IEE164 pKa = 4.38DD165 pKa = 4.03AVSSANQLIADD176 pKa = 4.06IAVANASIAASPDD189 pKa = 3.32NNQLISDD196 pKa = 4.42RR197 pKa = 11.84DD198 pKa = 3.45QMIRR202 pKa = 11.84EE203 pKa = 4.31LNAIIGVQTIEE214 pKa = 4.17QEE216 pKa = 4.01NGMVTILTDD225 pKa = 3.21EE226 pKa = 5.19GYY228 pKa = 10.93SLVDD232 pKa = 3.24GTEE235 pKa = 4.04THH237 pKa = 6.19SLVYY241 pKa = 10.67GDD243 pKa = 4.32ATATSALVRR252 pKa = 11.84ASDD255 pKa = 3.83YY256 pKa = 11.31DD257 pKa = 3.72GEE259 pKa = 4.31LAYY262 pKa = 10.9SGTSSEE268 pKa = 4.13EE269 pKa = 3.72VLLKK273 pKa = 10.38FVSSGADD280 pKa = 3.04GTAQFKK286 pKa = 10.94ASLDD290 pKa = 3.71GGKK293 pKa = 7.63TWITDD298 pKa = 3.63EE299 pKa = 4.35NGEE302 pKa = 4.26TMLYY306 pKa = 9.93IAGDD310 pKa = 3.37EE311 pKa = 4.06AAPVEE316 pKa = 4.17IAGIEE321 pKa = 3.69IWFEE325 pKa = 4.02GGSADD330 pKa = 4.09HH331 pKa = 6.97AVGDD335 pKa = 4.35TYY337 pKa = 11.1TIVPKK342 pKa = 10.75SGLYY346 pKa = 8.9WEE348 pKa = 5.12KK349 pKa = 11.32SDD351 pKa = 4.16GSLVNITPMTDD362 pKa = 2.92SSGTAVNGRR371 pKa = 11.84TSGGSLAGLFNVRR384 pKa = 11.84DD385 pKa = 3.97DD386 pKa = 4.11VVTPTLDD393 pKa = 3.41SLNDD397 pKa = 3.68LSEE400 pKa = 4.66SIIWEE405 pKa = 4.12VNAVHH410 pKa = 5.8STGAGLDD417 pKa = 3.34HH418 pKa = 7.22HH419 pKa = 6.46EE420 pKa = 4.63TLTGSYY426 pKa = 8.53TVEE429 pKa = 4.07DD430 pKa = 3.65SGALLSNSGLHH441 pKa = 6.26FADD444 pKa = 4.02NVTAGDD450 pKa = 4.29FSLVTYY456 pKa = 10.63DD457 pKa = 3.18SDD459 pKa = 4.8GNVSTSAIISFDD471 pKa = 3.71PATDD475 pKa = 3.69SLDD478 pKa = 3.91DD479 pKa = 4.91LMAEE483 pKa = 4.17INTAFGGEE491 pKa = 4.04LTASINASGQFVLSAGSDD509 pKa = 3.3TGFEE513 pKa = 4.14IASDD517 pKa = 3.86STGLMAALGVNTFFSGIDD535 pKa = 3.38ADD537 pKa = 4.51TIAIASAVAEE547 pKa = 4.1DD548 pKa = 3.81TSRR551 pKa = 11.84INAGAVGSDD560 pKa = 3.38GLVSSGNNDD569 pKa = 2.77IATLLAALSDD579 pKa = 4.03SKK581 pKa = 11.71VGVGDD586 pKa = 4.05KK587 pKa = 9.46NTSLTSYY594 pKa = 9.97LASLVAGVGAAASSAEE610 pKa = 4.13LKK612 pKa = 9.4LTYY615 pKa = 10.44AQTSVDD621 pKa = 3.78YY622 pKa = 10.64YY623 pKa = 10.45YY624 pKa = 10.51QQQASASEE632 pKa = 4.29VNVDD636 pKa = 3.58EE637 pKa = 5.11EE638 pKa = 5.41LINLLKK644 pKa = 10.48YY645 pKa = 9.84QQAFKK650 pKa = 10.65AAAEE654 pKa = 4.12IMTVTRR660 pKa = 11.84TMMDD664 pKa = 3.16TVLDD668 pKa = 3.86IVV670 pKa = 3.82

Molecular weight: 69.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6VRP6|E6VRP6_PSEA9 Heat shock protein Hsp20 OS=Pseudodesulfovibrio aespoeensis (strain ATCC 700646 / DSM 10631 / Aspo-2) OX=643562 GN=Daes_3191 PE=3 SV=1
MM1 pKa = 7.7PKK3 pKa = 9.95IKK5 pKa = 9.47TRR7 pKa = 11.84RR8 pKa = 11.84AAAKK12 pKa = 9.63RR13 pKa = 11.84FSKK16 pKa = 9.46TASGKK21 pKa = 9.4FKK23 pKa = 10.67RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84KK27 pKa = 9.07NLRR30 pKa = 11.84HH31 pKa = 6.24ILTKK35 pKa = 10.67KK36 pKa = 7.68NAKK39 pKa = 9.22RR40 pKa = 11.84KK41 pKa = 9.18RR42 pKa = 11.84RR43 pKa = 11.84LGQSTLVDD51 pKa = 3.93STNMKK56 pKa = 9.88AVRR59 pKa = 11.84RR60 pKa = 11.84QLPNGG65 pKa = 3.51

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3269

0

3269

1066561

31

3450

326.3

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.298 ± 0.057

1.314 ± 0.019

5.837 ± 0.04

6.127 ± 0.036

3.998 ± 0.03

8.346 ± 0.047

2.106 ± 0.022

5.251 ± 0.035

4.061 ± 0.04

10.429 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.893 ± 0.019

2.888 ± 0.029

4.778 ± 0.032

3.124 ± 0.023

6.605 ± 0.04

5.419 ± 0.036

5.371 ± 0.045

7.439 ± 0.038

1.167 ± 0.016

2.55 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski