Helcococcus kunzii ATCC 51366

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Helcococcus; Helcococcus kunzii

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1876 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H3NQT2|H3NQT2_9FIRM Sortase B cell surface sorting signal OS=Helcococcus kunzii ATCC 51366 OX=883114 GN=HMPREF9709_01693 PE=4 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84NYY4 pKa = 10.46EE5 pKa = 4.13VFNTEE10 pKa = 3.77QEE12 pKa = 4.18LLDD15 pKa = 5.6RR16 pKa = 11.84IQQLQEE22 pKa = 3.19TGYY25 pKa = 9.98QYY27 pKa = 11.44SEE29 pKa = 4.56FEE31 pKa = 4.49VYY33 pKa = 9.4TSNEE37 pKa = 3.63LSEE40 pKa = 4.7DD41 pKa = 3.29SEE43 pKa = 4.92YY44 pKa = 11.24YY45 pKa = 10.7YY46 pKa = 10.99DD47 pKa = 3.9VNKK50 pKa = 10.44HH51 pKa = 6.22DD52 pKa = 4.59GEE54 pKa = 4.31ASFVDD59 pKa = 3.93KK60 pKa = 10.95VAAFFTGEE68 pKa = 4.06DD69 pKa = 3.42PEE71 pKa = 4.1EE72 pKa = 4.19RR73 pKa = 11.84VFSKK77 pKa = 10.91YY78 pKa = 9.32EE79 pKa = 3.48WDD81 pKa = 3.6EE82 pKa = 4.13DD83 pKa = 3.83SKK85 pKa = 11.32EE86 pKa = 3.99QAKK89 pKa = 10.18RR90 pKa = 11.84AIDD93 pKa = 3.34NGLYY97 pKa = 10.39VLVVDD102 pKa = 4.97RR103 pKa = 11.84EE104 pKa = 4.55GYY106 pKa = 9.7YY107 pKa = 10.63EE108 pKa = 3.94NEE110 pKa = 3.74EE111 pKa = 3.93NFRR114 pKa = 11.84YY115 pKa = 10.09LDD117 pKa = 3.65NPEE120 pKa = 4.05NRR122 pKa = 11.84EE123 pKa = 3.24NDD125 pKa = 3.88YY126 pKa = 11.38INNPEE131 pKa = 4.09YY132 pKa = 10.82TGDD135 pKa = 3.72GYY137 pKa = 11.99VNNPEE142 pKa = 4.06YY143 pKa = 11.13NRR145 pKa = 11.84DD146 pKa = 3.41DD147 pKa = 4.17VIADD151 pKa = 3.57KK152 pKa = 10.99EE153 pKa = 4.23IGNEE157 pKa = 4.04SPTKK161 pKa = 9.73KK162 pKa = 9.99NRR164 pKa = 11.84RR165 pKa = 11.84EE166 pKa = 3.98LTDD169 pKa = 3.48EE170 pKa = 5.16DD171 pKa = 4.09EE172 pKa = 5.32LSVEE176 pKa = 4.7NYY178 pKa = 8.93TLYY181 pKa = 11.22NEE183 pKa = 4.9DD184 pKa = 5.53DD185 pKa = 3.89EE186 pKa = 5.14EE187 pKa = 5.59LDD189 pKa = 3.66YY190 pKa = 11.72DD191 pKa = 4.57EE192 pKa = 6.34EE193 pKa = 5.19EE194 pKa = 5.38DD195 pKa = 5.24DD196 pKa = 5.45LLL198 pKa = 6.29

Molecular weight: 23.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H3NQU7|H3NQU7_9FIRM R3H domain-containing protein OS=Helcococcus kunzii ATCC 51366 OX=883114 GN=HMPREF9709_01708 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.23QPNRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 8.46SKK14 pKa = 10.02KK15 pKa = 8.2HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTPGGKK28 pKa = 8.9RR29 pKa = 11.84VLQARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.52GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1876

0

1876

604950

25

3160

322.5

36.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.189 ± 0.056

0.54 ± 0.016

6.176 ± 0.061

7.823 ± 0.061

4.771 ± 0.043

5.929 ± 0.064

1.36 ± 0.02

9.87 ± 0.076

9.187 ± 0.064

8.973 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.03

6.652 ± 0.063

2.622 ± 0.033

2.529 ± 0.027

3.357 ± 0.035

6.372 ± 0.039

4.987 ± 0.047

6.071 ± 0.046

0.733 ± 0.026

4.424 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski