Yellowstone Lake virophage 5

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; unclassified Lavidaviridae

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0RJS4|A0A0A0RJS4_9VIRU Uncharacterized protein OS=Yellowstone Lake virophage 5 OX=1557033 GN=YSLV5_ORF08 PE=4 SV=1
MM1 pKa = 7.39SSVASILTGTPAVVNPALVSGGGGGGSTAPQFTATGVAGAGGFVSSGAGANVTLNNGTFVSYY63 pKa = 11.32VDD65 pKa = 3.85TLAVPPGGAEE75 pKa = 4.07VVVLGSDD82 pKa = 3.24VGGNGPRR89 pKa = 11.84WTIGYY94 pKa = 9.24NNSDD98 pKa = 3.53VGDD101 pKa = 4.11GFAGADD107 pKa = 3.75LAVKK111 pKa = 10.12CYY113 pKa = 10.79ADD115 pKa = 4.88DD116 pKa = 4.52GNLIATPFSIARR128 pKa = 11.84DD129 pKa = 3.61TGIVSMPEE137 pKa = 3.73SFEE140 pKa = 4.06ASAGEE145 pKa = 4.26VGAPATVGGGVLNINGTLGLSRR167 pKa = 11.84VYY169 pKa = 10.51DD170 pKa = 3.31QLYY173 pKa = 9.99NVPPATAQGAYY184 pKa = 10.14AFQSTKK190 pKa = 10.41LAASTFVDD198 pKa = 5.81FISNGNSTIITAPVPVGAQNASIFKK223 pKa = 10.18LRR225 pKa = 11.84VVGGWTYY232 pKa = 9.17ATPSGFIANMAIYY245 pKa = 10.02GATDD249 pKa = 3.73PAALVPDD256 pKa = 4.5DD257 pKa = 4.1TSMDD261 pKa = 3.34TVMVYY266 pKa = 10.73DD267 pKa = 4.43VFSGDD272 pKa = 3.46QIRR275 pKa = 11.84FEE277 pKa = 4.56VNPHH281 pKa = 5.94FLVGSEE287 pKa = 3.97AVSLVYY293 pKa = 10.31LRR295 pKa = 11.84EE296 pKa = 3.97MVFTVTNPTLATVTNLYY313 pKa = 10.5LVVRR317 pKa = 11.84PTPADD322 pKa = 3.57GSQTIGSVTTDD333 pKa = 2.93NGGPTYY339 pKa = 10.78ANCEE343 pKa = 4.0AYY345 pKa = 10.49LL346 pKa = 3.88

Molecular weight: 34.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0RPA2|A0A0A0RPA2_9VIRU Uncharacterized protein OS=Yellowstone Lake virophage 5 OX=1557033 GN=YSLV5_ORF04 PE=4 SV=1
MM1 pKa = 7.84GYY3 pKa = 10.48LCFLLPLLPLGQPFLVVIPRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84IRR27 pKa = 11.84PIKK30 pKa = 9.66VSHH33 pKa = 6.43SGFGTTTSSSHH44 pKa = 6.37SEE46 pKa = 4.07EE47 pKa = 4.01AKK49 pKa = 10.69LPYY52 pKa = 9.84KK53 pKa = 9.73SLRR56 pKa = 11.84RR57 pKa = 11.84NFSAHH62 pKa = 5.32

Molecular weight: 7.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32

0

32

8954

62

904

279.8

31.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.745 ± 0.413

1.284 ± 0.251

5.696 ± 0.321

6.243 ± 0.586

3.864 ± 0.203

7.807 ± 0.598

1.619 ± 0.2

5.149 ± 0.313

7.058 ± 0.839

7.628 ± 0.428

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.602 ± 0.195

4.177 ± 0.328

5.484 ± 0.374

2.937 ± 0.23

5.461 ± 0.483

6.768 ± 0.514

5.997 ± 0.573

6.299 ± 0.371

1.273 ± 0.147

3.909 ± 0.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski