Streptococcus sp. WM07
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1709 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y9FGU6|A0A4Y9FGU6_9STRE Uncharacterized protein OS=Streptococcus sp. WM07 OX=2558284 GN=E4T71_04790 PE=4 SV=1
MM1 pKa = 8.29 DD2 pKa = 6.39 DD3 pKa = 3.53 LTSLFGVTGSQGNVLDD19 pKa = 3.97 TFEE22 pKa = 4.57 EE23 pKa = 4.46 FAEE26 pKa = 4.21 EE27 pKa = 4.16 AVAEE31 pKa = 4.41 TEE33 pKa = 4.48 KK34 pKa = 11.3 NPSEE38 pKa = 4.1 KK39 pKa = 10.43 NAAFSQLLIDD49 pKa = 4.33 ALEE52 pKa = 4.42 DD53 pKa = 3.63 SEE55 pKa = 5.55 FRR57 pKa = 11.84 TGLQLRR63 pKa = 11.84 QDD65 pKa = 4.12 TVWPRR70 pKa = 11.84 EE71 pKa = 4.08 GVVYY75 pKa = 9.64 AQPVAVEE82 pKa = 4.04 ASAPVAEE89 pKa = 4.35 VAPLSFEE96 pKa = 4.76 AVATPAAPVVEE107 pKa = 4.95 EE108 pKa = 3.97 VAEE111 pKa = 4.22 PVAAPVAEE119 pKa = 4.31 VASPVVAAPVSEE131 pKa = 4.0 PAAPVEE137 pKa = 4.06 ATPVSTEE144 pKa = 3.48 AVAAEE149 pKa = 4.21 VVNLVSSAPAAPVSEE164 pKa = 4.08 AAAPVVEE171 pKa = 4.06 ASEE174 pKa = 4.08 VSEE177 pKa = 4.25 AVEE180 pKa = 4.43 TPAVEE185 pKa = 4.27 STQGSEE191 pKa = 3.85 SAAQVEE197 pKa = 5.05 GEE199 pKa = 4.18 HH200 pKa = 5.91 QAAEE204 pKa = 3.96 QAAAQAEE211 pKa = 4.51 AEE213 pKa = 4.18 RR214 pKa = 11.84 QAAEE218 pKa = 3.76 QAAAQAEE225 pKa = 4.51 AEE227 pKa = 4.18 RR228 pKa = 11.84 QAAEE232 pKa = 3.76 QAAAQAEE239 pKa = 4.51 AEE241 pKa = 4.18 RR242 pKa = 11.84 QAAEE246 pKa = 3.72 QAAAEE251 pKa = 4.23 QAAQAQTGSITPTYY265 pKa = 10.44 NYY267 pKa = 7.37 NTSGSYY273 pKa = 10.1 PVGQCTWGVKK283 pKa = 8.95 VMAPWVGDD291 pKa = 3.29 YY292 pKa = 9.65 WGNGGDD298 pKa = 3.59 WAASAAAAGFRR309 pKa = 11.84 TGTTPQVGAVASWNDD324 pKa = 3.03 GGYY327 pKa = 10.59 GHH329 pKa = 6.79 VAYY332 pKa = 9.36 VTAVEE337 pKa = 4.56 SNNAIQVYY345 pKa = 8.46 EE346 pKa = 4.19 SNYY349 pKa = 10.93 NGIQQIGNYY358 pKa = 9.55 RR359 pKa = 11.84 GTFDD363 pKa = 3.47 PTTAQGTVTYY373 pKa = 9.88 IYY375 pKa = 10.34 PNN377 pKa = 3.33
Molecular weight: 38.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.872
IPC_protein 3.808
Toseland 3.643
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.567
Rodwell 3.643
Grimsley 3.554
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 3.923
Thurlkill 3.656
EMBOSS 3.592
Sillero 3.91
Patrickios 1.125
IPC_peptide 3.732
IPC2_peptide 3.897
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A4Y9FHG4|A0A4Y9FHG4_9STRE Uncharacterized protein OS=Streptococcus sp. WM07 OX=2558284 GN=E4T71_06235 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.67 IRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 KK15 pKa = 7.52 HH16 pKa = 5.2 GFRR19 pKa = 11.84 HH20 pKa = 6.39 RR21 pKa = 11.84 MSTKK25 pKa = 9.02 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1709
0
1709
496822
23
2629
290.7
32.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.98 ± 0.11
0.465 ± 0.017
5.548 ± 0.061
7.457 ± 0.076
4.241 ± 0.057
6.788 ± 0.066
1.834 ± 0.03
6.365 ± 0.06
5.868 ± 0.075
10.562 ± 0.122
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.374 ± 0.033
3.861 ± 0.042
3.783 ± 0.052
4.853 ± 0.061
4.545 ± 0.051
6.265 ± 0.136
5.438 ± 0.07
7.217 ± 0.083
0.973 ± 0.021
3.582 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here