Klebsiella phage ST13-OXA48phi12.5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8RNZ5|A0A5B8RNZ5_9CAUD Tail protein OS=Klebsiella phage ST13-OXA48phi12.5 OX=2594282 PE=4 SV=1
MM1 pKa = 7.47SSVANWSYY9 pKa = 9.65TATATIWRR17 pKa = 11.84RR18 pKa = 11.84IRR20 pKa = 11.84DD21 pKa = 3.67ADD23 pKa = 4.18GSDD26 pKa = 3.52TDD28 pKa = 5.45GGGQPYY34 pKa = 10.43GWGPPIAILCDD45 pKa = 3.71YY46 pKa = 10.98QGGLSAKK53 pKa = 9.72IGDD56 pKa = 3.66IGRR59 pKa = 11.84EE60 pKa = 3.59IVVKK64 pKa = 8.98NTIWTEE70 pKa = 3.69YY71 pKa = 8.62ATARR75 pKa = 11.84EE76 pKa = 3.91GDD78 pKa = 4.08YY79 pKa = 11.07ILIGASTDD87 pKa = 3.27AAPPDD92 pKa = 3.57EE93 pKa = 5.39ADD95 pKa = 4.07EE96 pKa = 4.21IRR98 pKa = 11.84QIVQFADD105 pKa = 3.27TFEE108 pKa = 4.42RR109 pKa = 11.84LADD112 pKa = 4.07DD113 pKa = 4.15FALITGVV120 pKa = 3.19

Molecular weight: 12.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8RRL9|A0A5B8RRL9_9CAUD Replication protein OS=Klebsiella phage ST13-OXA48phi12.5 OX=2594282 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 8.66RR3 pKa = 11.84TPFYY7 pKa = 10.64RR8 pKa = 11.84RR9 pKa = 11.84PGKK12 pKa = 10.06AGKK15 pKa = 10.12FSGLRR20 pKa = 11.84EE21 pKa = 3.79RR22 pKa = 11.84VIWMIQTRR30 pKa = 11.84GRR32 pKa = 11.84PVTGSEE38 pKa = 3.59IAEE41 pKa = 4.27KK42 pKa = 10.62FGVTLVEE49 pKa = 4.59FNRR52 pKa = 11.84VANGITRR59 pKa = 11.84GEE61 pKa = 3.86GRR63 pKa = 11.84IAQLIASEE71 pKa = 4.18TWLNEE76 pKa = 4.2DD77 pKa = 4.91GICDD81 pKa = 3.54RR82 pKa = 11.84TFDD85 pKa = 5.15LITRR89 pKa = 11.84PKK91 pKa = 10.64VITPQGKK98 pKa = 7.26TRR100 pKa = 11.84LFTKK104 pKa = 10.49RR105 pKa = 11.84SIAQAASGNRR115 pKa = 11.84QKK117 pKa = 11.15CIDD120 pKa = 3.14KK121 pKa = 9.97AARR124 pKa = 11.84RR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84LIASGLYY134 pKa = 8.29IDD136 pKa = 4.96EE137 pKa = 4.61MEE139 pKa = 4.47SVLL142 pKa = 5.09

Molecular weight: 16.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

13500

35

889

190.1

21.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.926 ± 0.54

1.319 ± 0.19

5.919 ± 0.258

6.348 ± 0.355

3.452 ± 0.158

7.222 ± 0.291

1.941 ± 0.187

5.644 ± 0.258

5.319 ± 0.322

7.63 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.165

4.607 ± 0.233

3.963 ± 0.234

4.111 ± 0.375

6.015 ± 0.382

6.77 ± 0.328

5.793 ± 0.357

6.585 ± 0.283

1.719 ± 0.186

3.163 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski