Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) (Burkholderia phymatum)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia; Paraburkholderia phymatum

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7461 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2JL75|B2JL75_PARP8 Aldehyde Dehydrogenase OS=Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) OX=391038 GN=Bphy_4949 PE=3 SV=1
MM1 pKa = 7.87DD2 pKa = 6.2SIVIAATGDD11 pKa = 3.49SEE13 pKa = 5.08EE14 pKa = 4.35PVTVDD19 pKa = 3.11MAKK22 pKa = 9.59AHH24 pKa = 6.3CRR26 pKa = 11.84VDD28 pKa = 3.97VDD30 pKa = 5.22DD31 pKa = 6.28DD32 pKa = 4.11DD33 pKa = 6.26ALFEE37 pKa = 4.87SIYY40 pKa = 10.59IPAARR45 pKa = 11.84QTVEE49 pKa = 3.84QYY51 pKa = 10.47TGLTLLSCDD60 pKa = 3.24VDD62 pKa = 4.44VLVCQCDD69 pKa = 3.51VTQNVIAVPVQPVASVTALATVDD92 pKa = 3.5ANGVQTPLDD101 pKa = 3.84LDD103 pKa = 3.84EE104 pKa = 5.09YY105 pKa = 10.17GIAVAKK111 pKa = 10.5VGMRR115 pKa = 11.84SVIVARR121 pKa = 11.84NGPLPSAPYY130 pKa = 10.62YY131 pKa = 9.66RR132 pKa = 11.84VQYY135 pKa = 8.8VAGYY139 pKa = 10.05AEE141 pKa = 4.8NATPPNLLLAILEE154 pKa = 4.51YY155 pKa = 11.18VGDD158 pKa = 3.86AYY160 pKa = 10.3EE161 pKa = 4.15NRR163 pKa = 11.84EE164 pKa = 4.08AQQTSQAINDD174 pKa = 3.5NPRR177 pKa = 11.84AVRR180 pKa = 11.84LMDD183 pKa = 4.03PFRR186 pKa = 11.84ITFGLL191 pKa = 3.66

Molecular weight: 20.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2JK81|B2JK81_PARP8 2-hydroxy-3-oxopropionate reductase OS=Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) OX=391038 GN=Bphy_1622 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7461

0

7461

2416160

30

4384

323.8

35.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.525 ± 0.045

1.009 ± 0.009

5.519 ± 0.025

5.105 ± 0.028

3.686 ± 0.021

8.04 ± 0.032

2.398 ± 0.016

4.751 ± 0.019

3.155 ± 0.025

10.028 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.426 ± 0.014

2.87 ± 0.029

4.977 ± 0.019

3.605 ± 0.019

7.065 ± 0.032

5.813 ± 0.025

5.495 ± 0.025

7.748 ± 0.023

1.367 ± 0.012

2.419 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski