Blochmannia floridanus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; ant endosymbionts; Candidatus Blochmannia

Average proteome isoelectric point is 7.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 583 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7VR24|Q7VR24_BLOFL DNA polymerase III subunit delta' OS=Blochmannia floridanus OX=203907 GN=holB PE=4 SV=1
MM1 pKa = 7.48SIVEE5 pKa = 4.53EE6 pKa = 4.11KK7 pKa = 10.77VKK9 pKa = 10.18TIIAEE14 pKa = 3.96QLGVKK19 pKa = 9.13QEE21 pKa = 4.05EE22 pKa = 4.95VVNHH26 pKa = 6.54ASFVDD31 pKa = 3.91DD32 pKa = 5.57LGADD36 pKa = 3.42SLDD39 pKa = 3.64TVEE42 pKa = 5.9LVMALEE48 pKa = 4.32EE49 pKa = 4.24EE50 pKa = 4.31FDD52 pKa = 3.79IEE54 pKa = 4.81IPDD57 pKa = 3.67EE58 pKa = 4.11EE59 pKa = 4.53AEE61 pKa = 5.03KK62 pKa = 9.69ITTVQAAIDD71 pKa = 4.51FIHH74 pKa = 6.61TNQKK78 pKa = 10.13

Molecular weight: 8.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q7VQV3|MNME_BLOFL tRNA modification GTPase MnmE OS=Blochmannia floridanus OX=203907 GN=mnmE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVLKK11 pKa = 10.65RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 7.22GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTQNGRR28 pKa = 11.84QILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.6GRR39 pKa = 11.84IRR41 pKa = 11.84LIVSSS46 pKa = 4.03

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

583

0

583

195064

38

1420

334.6

38.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.566 ± 0.081

1.578 ± 0.037

4.585 ± 0.068

4.439 ± 0.075

4.453 ± 0.076

5.7 ± 0.096

2.581 ± 0.063

11.407 ± 0.118

7.168 ± 0.081

10.051 ± 0.102

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.508 ± 0.047

6.889 ± 0.108

3.18 ± 0.048

3.9 ± 0.067

4.082 ± 0.074

7.029 ± 0.064

4.864 ± 0.068

5.751 ± 0.12

1.037 ± 0.034

4.232 ± 0.069
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski