Cyclospora cayetanensis
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7151 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D3CS24|A0A1D3CS24_9EIME Uncharacterized protein OS=Cyclospora cayetanensis OX=88456 GN=cyc_07968 PE=4 SV=1
MM1 pKa = 7.44 TPPLRR6 pKa = 11.84 GSPTGRR12 pKa = 11.84 LSAAAAAIATAAMSALLLSPSAKK35 pKa = 9.86 AQEE38 pKa = 4.58 PYY40 pKa = 10.53 GVPSEE45 pKa = 4.87 ASWAPASASSTLSYY59 pKa = 10.81 SPSAFSASASPSAYY73 pKa = 9.3 ISPSLSFSASSSYY86 pKa = 11.11 QYY88 pKa = 11.13 TNYY91 pKa = 10.09 PEE93 pKa = 4.11 YY94 pKa = 10.08 QGSGGGPQVASEE106 pKa = 4.19 LSGLGYY112 pKa = 8.37 FTASLDD118 pKa = 3.42 TRR120 pKa = 11.84 AISAGQYY127 pKa = 9.08 SASPEE132 pKa = 4.23 TFSVSPSSSIPAFDD146 pKa = 3.74 SFTPYY151 pKa = 10.71 APPAAPSFAPSAAPPFAPSAAPSSAAAYY179 pKa = 9.52 QDD181 pKa = 3.79 AGIATTEE188 pKa = 3.98 ATATAASAYY197 pKa = 8.26 EE198 pKa = 4.34 AYY200 pKa = 10.45 ASGGLTASSGSPYY213 pKa = 10.03 TPASNPVSPSYY224 pKa = 10.7 FDD226 pKa = 3.69 EE227 pKa = 5.75 GPTEE231 pKa = 4.12 MAPLPSPSMPVAGVAEE247 pKa = 4.7 AAAEE251 pKa = 4.1 MQTAAVAAVAADD263 pKa = 3.92 TTATADD269 pKa = 3.39 MAEE272 pKa = 4.09 AAVAAEE278 pKa = 4.06 AAAAALEE285 pKa = 4.09 ALQEE289 pKa = 4.4 TLTSPTQHH297 pKa = 6.71 HH298 pKa = 6.53 EE299 pKa = 3.98 ATTEE303 pKa = 3.77 AVANTEE309 pKa = 4.13 QVDD312 pKa = 3.43 SHH314 pKa = 6.72 AAEE317 pKa = 4.01 TVAAQGAPEE326 pKa = 4.85 HH327 pKa = 6.59 PPSVQVSPTADD338 pKa = 3.18 SSPDD342 pKa = 3.45 TVSAEE347 pKa = 4.06 TTAARR352 pKa = 11.84 QPTEE356 pKa = 3.94 SSEE359 pKa = 3.96 AAQQEE364 pKa = 4.66 GAEE367 pKa = 4.36 SARR370 pKa = 11.84 VQDD373 pKa = 3.65 EE374 pKa = 4.11 ASAVALTEE382 pKa = 3.81 PTAAQTPSEE391 pKa = 4.52 GQQHH395 pKa = 5.58 TPVEE399 pKa = 4.49 EE400 pKa = 3.95 QTQQQGEE407 pKa = 4.34 TAEE410 pKa = 4.4 GLLTAAAGDD419 pKa = 4.19 TYY421 pKa = 11.63 VEE423 pKa = 4.46 TPAAASTTQSAEE435 pKa = 3.57 VDD437 pKa = 3.51 AGGHH441 pKa = 4.29 TTLAEE446 pKa = 4.2 GTTALPSEE454 pKa = 4.44 AVMPLAEE461 pKa = 4.2 AAEE464 pKa = 4.26 AQHH467 pKa = 6.68 ASSHH471 pKa = 6.52 PSQSATGEE479 pKa = 3.93 ASEE482 pKa = 5.31 DD483 pKa = 3.44 QQQQSQQPNEE493 pKa = 4.05 PPQLQQATEE502 pKa = 3.69 THH504 pKa = 6.16 AAYY507 pKa = 9.59 EE508 pKa = 4.31 SLGALPNAHH517 pKa = 6.35 PQEE520 pKa = 4.52 EE521 pKa = 5.3 GSTEE525 pKa = 4.09 GAPTGAATPPSSLSAAVDD543 pKa = 3.61 EE544 pKa = 4.85 AVMFAAAINAPSSAPAEE561 pKa = 4.23 AGSADD566 pKa = 3.71 EE567 pKa = 5.52 AEE569 pKa = 4.5 SQAAATQGGMLPEE582 pKa = 5.14 SYY584 pKa = 10.53 PEE586 pKa = 4.58 GPTLEE591 pKa = 4.4 TPLEE595 pKa = 4.02 EE596 pKa = 4.1 MMMEE600 pKa = 4.54 HH601 pKa = 6.75 AQNNPAAATQAEE613 pKa = 4.64 EE614 pKa = 4.97 DD615 pKa = 3.55 SQGPHH620 pKa = 6.5 RR621 pKa = 11.84 EE622 pKa = 4.08 SEE624 pKa = 4.27 AAGAAPAADD633 pKa = 3.94 GQLDD637 pKa = 3.67 AAASEE642 pKa = 4.29 PTQRR646 pKa = 11.84 GALDD650 pKa = 3.49 TLTGVTQQAIAFGSHH665 pKa = 7.12 PEE667 pKa = 4.12 SEE669 pKa = 4.28 TDD671 pKa = 3.19 EE672 pKa = 4.38 TAEE675 pKa = 4.31 SQQQQHH681 pKa = 7.1 PGQSQQQRR689 pKa = 11.84 GQGTLEE695 pKa = 4.04 QEE697 pKa = 4.39 ASAQQHH703 pKa = 6.08 EE704 pKa = 4.73 IVIRR708 pKa = 11.84 LLTGDD713 pKa = 4.58 KK714 pKa = 10.53 EE715 pKa = 4.23 DD716 pKa = 4.21 LAASLSRR723 pKa = 11.84 LAEE726 pKa = 4.42 TIRR729 pKa = 11.84 HH730 pKa = 5.79 RR731 pKa = 11.84 EE732 pKa = 3.66 RR733 pKa = 11.84 LANPHH738 pKa = 6.41 EE739 pKa = 4.28 EE740 pKa = 4.04 LATVEE745 pKa = 4.13 GTEE748 pKa = 4.24 QQEE751 pKa = 4.38 QQQTPADD758 pKa = 3.55 ATEE761 pKa = 4.05 NAEE764 pKa = 4.38 GVAAPLKK771 pKa = 10.39 PGTPQEE777 pKa = 4.26 EE778 pKa = 4.46 EE779 pKa = 3.64 QDD781 pKa = 3.6 QSGEE785 pKa = 4.12 EE786 pKa = 4.11 TPDD789 pKa = 3.08 RR790 pKa = 11.84 SRR792 pKa = 11.84 QYY794 pKa = 10.39 IFQSVSQQQQQDD806 pKa = 3.33 LPAEE810 pKa = 4.32 VEE812 pKa = 3.96 ALQQVLIQQQLQQTHH827 pKa = 4.47 QQVLLEE833 pKa = 4.21 EE834 pKa = 4.28 EE835 pKa = 4.67 LNGSVEE841 pKa = 4.25 VPDD844 pKa = 3.51 IEE846 pKa = 6.46 AYY848 pKa = 9.74 VQQ850 pKa = 3.24
Molecular weight: 86.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.777
IPC2_protein 3.961
IPC_protein 3.91
Toseland 3.757
ProMoST 3.999
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.681
Rodwell 3.745
Grimsley 3.668
Solomon 3.846
Lehninger 3.795
Nozaki 3.948
DTASelect 4.05
Thurlkill 3.757
EMBOSS 3.706
Sillero 4.012
Patrickios 1.481
IPC_peptide 3.846
IPC2_peptide 4.012
IPC2.peptide.svr19 3.905
Protein with the highest isoelectric point:
>tr|A0A1D3CZ42|A0A1D3CZ42_9EIME Vacuolar protein sorting OS=Cyclospora cayetanensis OX=88456 GN=cyc_05819 PE=4 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 GHH4 pKa = 6.73 CTPGDD9 pKa = 3.17 WAYY12 pKa = 11.56 VMEE15 pKa = 4.66 VSDD18 pKa = 5.1 PEE20 pKa = 3.95 QQLRR24 pKa = 11.84 RR25 pKa = 11.84 FMRR28 pKa = 11.84 QAFSAEE34 pKa = 4.08 GGPLLNVLSRR44 pKa = 11.84 DD45 pKa = 3.96 RR46 pKa = 11.84 IQLWGSGRR54 pKa = 11.84 LRR56 pKa = 11.84 SPSSRR61 pKa = 11.84 RR62 pKa = 11.84 LAQASSRR69 pKa = 11.84 FCKK72 pKa = 10.48 GLFSRR77 pKa = 11.84 VPPRR81 pKa = 11.84 RR82 pKa = 11.84 RR83 pKa = 11.84 EE84 pKa = 3.85 GLRR87 pKa = 11.84 DD88 pKa = 3.67 SNASSSLPAPHH99 pKa = 7.29 EE100 pKa = 4.54 SPLMGPPKK108 pKa = 9.95 PPPAPQDD115 pKa = 3.75 SNMMRR120 pKa = 11.84 ASVHH124 pKa = 5.88 ARR126 pKa = 11.84 CLQDD130 pKa = 3.83 FYY132 pKa = 11.76 FRR134 pKa = 11.84 LEE136 pKa = 3.91 EE137 pKa = 4.11 EE138 pKa = 4.2 KK139 pKa = 11.09 APGYY143 pKa = 8.14 VLCICVGPPSRR154 pKa = 11.84 ALKK157 pKa = 9.91 EE158 pKa = 4.42 YY159 pKa = 9.15 KK160 pKa = 9.89 SCMPRR165 pKa = 11.84 VRR167 pKa = 11.84 LRR169 pKa = 11.84 GKK171 pKa = 9.99 GGRR174 pKa = 11.84 TGTVPP179 pKa = 3.58
Molecular weight: 20.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.545
IPC_protein 10.496
Toseland 10.599
ProMoST 10.379
Dawson 10.701
Bjellqvist 10.482
Wikipedia 10.95
Rodwell 10.774
Grimsley 10.76
Solomon 10.847
Lehninger 10.804
Nozaki 10.628
DTASelect 10.452
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.657
Patrickios 10.482
IPC_peptide 10.847
IPC2_peptide 9.853
IPC2.peptide.svr19 8.552
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7151
0
7151
3734096
66
8164
522.2
56.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.347 ± 0.045
2.293 ± 0.016
4.208 ± 0.015
6.733 ± 0.024
3.195 ± 0.016
7.023 ± 0.026
2.465 ± 0.012
3.064 ± 0.018
4.02 ± 0.023
10.602 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.746 ± 0.01
2.344 ± 0.013
6.221 ± 0.027
5.342 ± 0.03
6.851 ± 0.024
8.841 ± 0.029
4.904 ± 0.016
5.898 ± 0.021
1.132 ± 0.007
1.764 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here