Hubei tombus-like virus 12

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGX1|A0A1L3KGX1_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 12 OX=1923258 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 5.38KK3 pKa = 10.54ADD5 pKa = 4.6DD6 pKa = 3.56RR7 pKa = 11.84HH8 pKa = 5.38GTPRR12 pKa = 11.84PPASKK17 pKa = 9.17GCEE20 pKa = 3.61SHH22 pKa = 6.69RR23 pKa = 11.84RR24 pKa = 11.84LIRR27 pKa = 11.84IVPPIYY33 pKa = 10.57GLWHH37 pKa = 6.97CFTHH41 pKa = 6.79SNCVCNDD48 pKa = 4.52LIACSNRR55 pKa = 11.84VIGVVPAPTDD65 pKa = 3.22TGIKK69 pKa = 9.86RR70 pKa = 11.84LRR72 pKa = 11.84EE73 pKa = 4.01AMNQLWPYY81 pKa = 10.86RR82 pKa = 11.84AMTPLSLEE90 pKa = 3.71QSLASFKK97 pKa = 10.79GSKK100 pKa = 9.94LKK102 pKa = 10.66LYY104 pKa = 10.0QRR106 pKa = 11.84AYY108 pKa = 10.76DD109 pKa = 3.79SLLITPLNQRR119 pKa = 11.84DD120 pKa = 3.7GRR122 pKa = 11.84VKK124 pKa = 10.88AFVKK128 pKa = 10.59AEE130 pKa = 4.08KK131 pKa = 10.05FDD133 pKa = 3.63PTEE136 pKa = 4.19KK137 pKa = 10.63VNPDD141 pKa = 2.77PRR143 pKa = 11.84MIQARR148 pKa = 11.84DD149 pKa = 3.27PRR151 pKa = 11.84YY152 pKa = 9.76NLHH155 pKa = 6.52LAQYY159 pKa = 9.39LRR161 pKa = 11.84PLEE164 pKa = 4.28HH165 pKa = 6.59EE166 pKa = 4.63CYY168 pKa = 10.25SLRR171 pKa = 11.84RR172 pKa = 11.84HH173 pKa = 6.08GAPAIAKK180 pKa = 8.56CFNPVQRR187 pKa = 11.84ANAIMQKK194 pKa = 9.83WNMFEE199 pKa = 4.5NPVCFSLDD207 pKa = 3.17CSRR210 pKa = 11.84WDD212 pKa = 3.23KK213 pKa = 10.86HH214 pKa = 4.52VHH216 pKa = 5.8RR217 pKa = 11.84KK218 pKa = 9.14VLDD221 pKa = 3.72VEE223 pKa = 4.51HH224 pKa = 6.98EE225 pKa = 4.98FYY227 pKa = 10.85QRR229 pKa = 11.84WYY231 pKa = 8.43PGEE234 pKa = 4.17VQLEE238 pKa = 4.42TLLGMQKK245 pKa = 10.49VNICTTSNGVKK256 pKa = 9.07YY257 pKa = 8.65TVDD260 pKa = 3.54GGRR263 pKa = 11.84MSGDD267 pKa = 3.19MNTALGNVTLMCGMIYY283 pKa = 10.67GAMKK287 pKa = 10.31TFDD290 pKa = 3.42NCHH293 pKa = 6.28FEE295 pKa = 4.01WLDD298 pKa = 4.4DD299 pKa = 4.47GDD301 pKa = 4.18DD302 pKa = 3.63CLVFVEE308 pKa = 4.14QHH310 pKa = 6.04DD311 pKa = 4.3LARR314 pKa = 11.84VAADD318 pKa = 3.79LPRR321 pKa = 11.84LFLEE325 pKa = 4.52YY326 pKa = 10.52GQEE329 pKa = 3.91LKK331 pKa = 10.56IEE333 pKa = 4.53NIAMNPQDD341 pKa = 4.76IVFCQSKK348 pKa = 8.47LTYY351 pKa = 10.3NGQFWTMARR360 pKa = 11.84NWRR363 pKa = 11.84KK364 pKa = 9.47VLSQSCCGTKK374 pKa = 9.43HH375 pKa = 5.95WNDD378 pKa = 3.49PNMVPGMFGLIGDD391 pKa = 4.78CEE393 pKa = 4.3MALHH397 pKa = 6.7RR398 pKa = 11.84GIPILQAFAQRR409 pKa = 11.84LRR411 pKa = 11.84DD412 pKa = 3.49LSGGRR417 pKa = 11.84RR418 pKa = 11.84ARR420 pKa = 11.84MEE422 pKa = 4.19HH423 pKa = 6.41MDD425 pKa = 3.24SSFQYY430 pKa = 10.4RR431 pKa = 11.84VGSYY435 pKa = 9.38QLGDD439 pKa = 3.4IQSITPAEE447 pKa = 4.23VTEE450 pKa = 4.01EE451 pKa = 3.82ARR453 pKa = 11.84FEE455 pKa = 4.14FQRR458 pKa = 11.84TWGVDD463 pKa = 2.64IQTQIGIEE471 pKa = 3.71WHH473 pKa = 5.99LAQWTPGIVHH483 pKa = 7.1RR484 pKa = 11.84DD485 pKa = 3.33VGPEE489 pKa = 3.87LFSTDD494 pKa = 2.23WCQQLEE500 pKa = 4.27PGIPNPTVLL509 pKa = 4.72

Molecular weight: 58.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGX1|A0A1L3KGX1_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 12 OX=1923258 PE=4 SV=1
MM1 pKa = 7.57SKK3 pKa = 8.49PTRR6 pKa = 11.84RR7 pKa = 11.84THH9 pKa = 6.3WLPTATYY16 pKa = 9.42RR17 pKa = 11.84GRR19 pKa = 11.84RR20 pKa = 11.84GSKK23 pKa = 8.48TPSGPSSVASDD34 pKa = 4.08LPSTDD39 pKa = 3.16EE40 pKa = 4.34LRR42 pKa = 11.84SEE44 pKa = 4.51LSRR47 pKa = 11.84LEE49 pKa = 4.28EE50 pKa = 4.13EE51 pKa = 4.35AQKK54 pKa = 9.85VQPWTDD60 pKa = 3.15VEE62 pKa = 4.25VRR64 pKa = 11.84LLGFLRR70 pKa = 11.84GRR72 pKa = 11.84LYY74 pKa = 10.64GAKK77 pKa = 8.67RR78 pKa = 11.84TPATPQSLNLQGLNWCVKK96 pKa = 9.46NHH98 pKa = 6.1DD99 pKa = 4.14HH100 pKa = 6.73LGEE103 pKa = 4.19YY104 pKa = 8.2QHH106 pKa = 7.15PAAQAALVARR116 pKa = 11.84CVNVVLPPSLEE127 pKa = 3.93EE128 pKa = 4.33AGLIDD133 pKa = 3.74VYY135 pKa = 10.9QRR137 pKa = 11.84EE138 pKa = 4.49GEE140 pKa = 4.19KK141 pKa = 10.29IKK143 pKa = 11.1AFNNGLDD150 pKa = 3.48EE151 pKa = 4.46MGDD154 pKa = 3.37RR155 pKa = 11.84RR156 pKa = 11.84EE157 pKa = 3.97DD158 pKa = 3.66LRR160 pKa = 11.84WLVGLGGVTAAVAGTVAGLTGSRR183 pKa = 11.84VIGGGLLAAGIAGVGIYY200 pKa = 9.79GWSKK204 pKa = 10.26VRR206 pKa = 11.84DD207 pKa = 3.42ITRR210 pKa = 11.84RR211 pKa = 11.84AQAARR216 pKa = 11.84WYY218 pKa = 10.41

Molecular weight: 23.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

727

218

509

363.5

41.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.29 ± 1.398

2.751 ± 0.915

5.365 ± 0.617

5.502 ± 0.23

3.026 ± 1.052

8.116 ± 1.672

2.889 ± 0.526

4.264 ± 0.754

3.989 ± 0.159

9.354 ± 0.826

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.889 ± 0.983

3.989 ± 0.617

5.64 ± 0.296

4.952 ± 0.64

7.978 ± 0.826

4.952 ± 0.505

5.089 ± 0.436

6.465 ± 0.665

2.613 ± 0.069

2.889 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski