Georhizobium profundi
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4118 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S9B9X6|A0A3S9B9X6_9RHIZ Urea ABC transporter permease subunit UrtB OS=Georhizobium profundi OX=2341112 GN=urtB PE=4 SV=1
MM1 pKa = 7.54 APAAMAQDD9 pKa = 3.57 AAGMPEE15 pKa = 4.03 EE16 pKa = 4.46 TGDD19 pKa = 5.08 LITATATLEE28 pKa = 4.11 GDD30 pKa = 4.05 GIEE33 pKa = 4.37 GTVTLTEE40 pKa = 4.31 TPTGIVIIEE49 pKa = 4.07 IEE51 pKa = 4.14 ASGLEE56 pKa = 4.15 PGPHH60 pKa = 5.77 GVHH63 pKa = 5.8 LHH65 pKa = 5.16 EE66 pKa = 5.54 TGTCDD71 pKa = 4.38 AEE73 pKa = 4.81 GGHH76 pKa = 6.06 EE77 pKa = 3.87 AAGGHH82 pKa = 4.84 VSGDD86 pKa = 3.71 GEE88 pKa = 4.44 HH89 pKa = 7.13 GVLNPNGPHH98 pKa = 7.01 PGDD101 pKa = 3.69 LPNVHH106 pKa = 5.54 VQEE109 pKa = 5.18 DD110 pKa = 5.18 GIAHH114 pKa = 6.83 AEE116 pKa = 4.09 FFVSDD121 pKa = 3.37 IALSGDD127 pKa = 3.44 AGTVILDD134 pKa = 3.74 DD135 pKa = 5.37 DD136 pKa = 4.21 GAAFIIHH143 pKa = 7.1 AGADD147 pKa = 3.55 DD148 pKa = 4.44 YY149 pKa = 10.53 MTDD152 pKa = 3.61 PAGDD156 pKa = 3.44 SGDD159 pKa = 4.42 RR160 pKa = 11.84 IACGVIEE167 pKa = 4.41 AANN170 pKa = 3.31
Molecular weight: 16.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.834
IPC_protein 3.808
Toseland 3.617
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.643
Grimsley 3.528
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.126
Thurlkill 3.656
EMBOSS 3.732
Sillero 3.935
Patrickios 1.062
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.843
Protein with the highest isoelectric point:
>tr|A0A3S9AZD4|A0A3S9AZD4_9RHIZ LysR family transcriptional regulator OS=Georhizobium profundi OX=2341112 GN=D5400_01175 PE=3 SV=1
MM1 pKa = 7.51 TKK3 pKa = 9.11 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.84 LVRR13 pKa = 11.84 KK14 pKa = 9.15 RR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.42 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATAGGRR29 pKa = 11.84 KK30 pKa = 8.88 VLAARR35 pKa = 11.84 RR36 pKa = 11.84 ARR38 pKa = 11.84 GRR40 pKa = 11.84 KK41 pKa = 9.03 RR42 pKa = 11.84 LSAA45 pKa = 4.03
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4118
0
4118
1291389
28
2867
313.6
34.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.399 ± 0.048
0.78 ± 0.012
6.06 ± 0.037
6.023 ± 0.034
3.854 ± 0.021
8.474 ± 0.042
2.029 ± 0.02
5.609 ± 0.028
3.035 ± 0.03
9.821 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.621 ± 0.019
2.607 ± 0.025
4.895 ± 0.026
3.104 ± 0.022
7.066 ± 0.049
5.563 ± 0.027
5.34 ± 0.028
7.334 ± 0.034
1.229 ± 0.014
2.158 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here