Gordonia phage Turuncu

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KAQ7|A0A386KAQ7_9CAUD Helix-turn-helix DNA binding protein OS=Gordonia phage Turuncu OX=2315610 GN=61 PE=4 SV=1
MM1 pKa = 7.58ASSATVVEE9 pKa = 5.03FEE11 pKa = 4.32TEE13 pKa = 3.78FWIEE17 pKa = 3.59FGTRR21 pKa = 11.84QVVPVGTEE29 pKa = 3.37ILGRR33 pKa = 11.84RR34 pKa = 11.84LDD36 pKa = 3.47EE37 pKa = 5.01GYY39 pKa = 10.84LVVEE43 pKa = 4.54ARR45 pKa = 11.84CIYY48 pKa = 10.55DD49 pKa = 3.38YY50 pKa = 11.76APFSDD55 pKa = 5.05GYY57 pKa = 11.55APAFDD62 pKa = 4.43FQATVLEE69 pKa = 4.17ATLFGEE75 pKa = 4.44LLDD78 pKa = 4.21PVEE81 pKa = 4.73VTCTSSPDD89 pKa = 3.45GEE91 pKa = 4.47GARR94 pKa = 11.84ITTIKK99 pKa = 10.19EE100 pKa = 3.69

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KBF2|A0A386KBF2_9CAUD Head-to-tail adaptor OS=Gordonia phage Turuncu OX=2315610 GN=35 PE=4 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84TRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84GGSLNTRR14 pKa = 11.84KK15 pKa = 9.98KK16 pKa = 10.82GKK18 pKa = 10.05AKK20 pKa = 10.66NGAKK24 pKa = 9.07WGHH27 pKa = 5.7GFVPKK32 pKa = 10.29NAAARR37 pKa = 11.84KK38 pKa = 9.03LKK40 pKa = 10.66KK41 pKa = 10.41KK42 pKa = 10.3LDD44 pKa = 3.9RR45 pKa = 11.84NGKK48 pKa = 7.93RR49 pKa = 11.84RR50 pKa = 11.84KK51 pKa = 8.79RR52 pKa = 11.84TSQGYY57 pKa = 7.89PKK59 pKa = 10.48RR60 pKa = 3.9

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

21080

32

1802

224.3

24.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.251 ± 0.431

0.806 ± 0.105

6.988 ± 0.255

6.395 ± 0.292

3.093 ± 0.157

8.828 ± 0.356

2.04 ± 0.188

5.019 ± 0.146

4.042 ± 0.247

7.405 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.106

3.193 ± 0.193

5.403 ± 0.172

3.273 ± 0.148

7.31 ± 0.296

5.455 ± 0.211

6.347 ± 0.171

7.282 ± 0.166

2.016 ± 0.115

2.614 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski