Mycoplasma marinum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma

Average proteome isoelectric point is 7.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 986 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R0XVJ8|A0A4R0XVJ8_9MOLU Uncharacterized protein OS=Mycoplasma marinum OX=1937190 GN=C4B24_00560 PE=4 SV=1
MM1 pKa = 7.71EE2 pKa = 5.02NNKK5 pKa = 8.17NTNLDD10 pKa = 3.24EE11 pKa = 4.52DD12 pKa = 4.73RR13 pKa = 11.84IIEE16 pKa = 4.19VVLEE20 pKa = 4.18NGEE23 pKa = 4.11KK24 pKa = 10.73AEE26 pKa = 4.51GIILFTFEE34 pKa = 4.87ANGDD38 pKa = 3.73NFVLYY43 pKa = 9.76EE44 pKa = 3.9IQEE47 pKa = 3.98QLFAAKK53 pKa = 10.05VDD55 pKa = 3.79EE56 pKa = 4.12QGGLKK61 pKa = 10.19SVEE64 pKa = 3.92EE65 pKa = 4.73DD66 pKa = 2.77EE67 pKa = 4.22WSLIEE72 pKa = 4.9KK73 pKa = 10.32VYY75 pKa = 11.07EE76 pKa = 4.3EE77 pKa = 4.15YY78 pKa = 10.82QEE80 pKa = 4.38TYY82 pKa = 10.6VEE84 pKa = 4.15EE85 pKa = 6.19GEE87 pKa = 5.13DD88 pKa = 3.56EE89 pKa = 4.2

Molecular weight: 10.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R0XI61|A0A4R0XI61_9MOLU Uncharacterized protein (Fragment) OS=Mycoplasma marinum OX=1937190 GN=C4B24_04945 PE=4 SV=1
MM1 pKa = 7.39FKK3 pKa = 10.75KK4 pKa = 10.55LLLISLVIALIQIITGILMHH24 pKa = 6.6FNSIEE29 pKa = 3.82FRR31 pKa = 11.84DD32 pKa = 3.74VKK34 pKa = 10.82SYY36 pKa = 8.24HH37 pKa = 5.07TYY39 pKa = 8.82YY40 pKa = 9.99WISSGILLLIGALFCLFKK58 pKa = 10.73TRR60 pKa = 11.84LRR62 pKa = 11.84NVGIYY67 pKa = 9.83FSIIALNVIFLALGCKK83 pKa = 8.35NWIGIVVIVAWILLFIIFVSRR104 pKa = 11.84RR105 pKa = 11.84IIISKK110 pKa = 10.44RR111 pKa = 11.84II112 pKa = 3.42

Molecular weight: 12.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

986

0

986

335004

37

6201

339.8

38.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.597 ± 0.083

0.429 ± 0.017

5.068 ± 0.084

6.609 ± 0.109

5.155 ± 0.066

5.788 ± 0.072

1.534 ± 0.024

10.048 ± 0.09

10.766 ± 0.089

8.399 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.045

6.536 ± 0.085

2.801 ± 0.041

2.816 ± 0.035

3.096 ± 0.059

6.901 ± 0.071

5.695 ± 0.105

5.734 ± 0.06

1.128 ± 0.028

3.485 ± 0.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski