Drosophila navojoa (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae;

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A484AV08|A0A484AV08_DRONA Uncharacterized protein OS=Drosophila navojoa OX=7232 GN=AWZ03_013085 PE=4 SV=1
MM1 pKa = 7.45IMLMTMKK8 pKa = 10.72LMIRR12 pKa = 11.84IHH14 pKa = 6.69YY15 pKa = 7.5GTNNNNNNDD24 pKa = 3.9NDD26 pKa = 3.96NDD28 pKa = 3.96TDD30 pKa = 5.01NNDD33 pKa = 3.44NDD35 pKa = 3.98NDD37 pKa = 3.67IDD39 pKa = 4.57NEE41 pKa = 4.03KK42 pKa = 11.28DD43 pKa = 3.59NNNEE47 pKa = 3.83NYY49 pKa = 10.56EE50 pKa = 4.31MIIMIMCNVHH60 pKa = 6.85CNDD63 pKa = 3.27DD64 pKa = 3.68HH65 pKa = 7.99LYY67 pKa = 10.99NDD69 pKa = 3.81NDD71 pKa = 4.19SNN73 pKa = 4.18

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A484BK91|A0A484BK91_DRONA Uncharacterized protein OS=Drosophila navojoa OX=7232 GN=AWZ03_004292 PE=3 SV=1
MM1 pKa = 7.73KK2 pKa = 10.3IFIVLALLIAAAAALPQFGFGGRR25 pKa = 11.84PGFGGPGFGGPGFGKK40 pKa = 9.71PGFGGPGFGGPGFGRR55 pKa = 11.84PGFGGPGFGGPGFGGPGFGGRR76 pKa = 11.84PGFGGPGFNRR86 pKa = 11.84GGGGGSASSSASASSSAAGGGRR108 pKa = 11.84GGGASASASASSSAAGGGFRR128 pKa = 11.84GG129 pKa = 3.81

Molecular weight: 11.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15505

0

15505

7239635

8

16766

466.9

52.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.089 ± 0.031

1.93 ± 0.034

5.089 ± 0.018

6.227 ± 0.024

3.324 ± 0.015

5.674 ± 0.023

2.676 ± 0.011

4.781 ± 0.019

5.34 ± 0.022

9.227 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.352 ± 0.011

4.94 ± 0.019

5.304 ± 0.027

5.906 ± 0.033

5.504 ± 0.016

8.22 ± 0.032

5.752 ± 0.02

5.645 ± 0.015

0.981 ± 0.007

3.021 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski