Cohnella sp. CIP 111063
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6817 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A231RMG5|A0A231RMG5_9BACL TGc domain-containing protein OS=Cohnella sp. CIP 111063 OX=1955714 GN=B1A99_08210 PE=4 SV=1
MM1 pKa = 6.91 VNKK4 pKa = 9.6 MLISLVAVLFGFMSFGSIQANTYY27 pKa = 10.15 EE28 pKa = 4.3 YY29 pKa = 10.69 TPVPKK34 pKa = 10.61 DD35 pKa = 3.19 EE36 pKa = 4.25 NTRR39 pKa = 11.84 VAYY42 pKa = 10.4 VGAIYY47 pKa = 10.47 QEE49 pKa = 4.34 DD50 pKa = 3.44 EE51 pKa = 4.6 GYY53 pKa = 10.92 YY54 pKa = 10.73 ADD56 pKa = 4.44 ADD58 pKa = 4.18 FIEE61 pKa = 4.95 WYY63 pKa = 10.04 EE64 pKa = 4.29 GEE66 pKa = 3.95 AANAIFRR73 pKa = 11.84 EE74 pKa = 4.22 RR75 pKa = 11.84 EE76 pKa = 3.67 ADD78 pKa = 3.41 SGMDD82 pKa = 3.62 EE83 pKa = 5.31 APDD86 pKa = 3.75 GYY88 pKa = 11.26 YY89 pKa = 9.85 IVNDD93 pKa = 3.51 DD94 pKa = 3.84 TTVEE98 pKa = 4.08 RR99 pKa = 11.84 LKK101 pKa = 10.65 LAPDD105 pKa = 3.6 AQVFMQIYY113 pKa = 10.46 DD114 pKa = 3.54 RR115 pKa = 11.84 TGDD118 pKa = 3.27 IAEE121 pKa = 5.3 ADD123 pKa = 4.25 TVWNEE128 pKa = 4.02 QISLDD133 pKa = 4.08 KK134 pKa = 10.85 FLALLADD141 pKa = 4.46 DD142 pKa = 4.35 SAMNLRR148 pKa = 11.84 DD149 pKa = 3.8 YY150 pKa = 10.29 PYY152 pKa = 10.56 HH153 pKa = 7.23 LSIVNGVVVKK163 pKa = 9.49 ITQQFLPP170 pKa = 4.19
Molecular weight: 19.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.935
IPC_protein 3.91
Toseland 3.706
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.745
Grimsley 3.617
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.228
Thurlkill 3.757
EMBOSS 3.834
Sillero 4.037
Patrickios 1.914
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.912
Protein with the highest isoelectric point:
>tr|A0A231RBY0|A0A231RBY0_9BACL Cyclic-di-AMP phosphodiesterase OS=Cohnella sp. CIP 111063 OX=1955714 GN=B1A99_20110 PE=3 SV=1
MM1 pKa = 7.61 GPTFKK6 pKa = 10.87 PNVSKK11 pKa = 10.8 RR12 pKa = 11.84 KK13 pKa = 8.57 KK14 pKa = 8.17 VHH16 pKa = 5.5 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSTKK25 pKa = 10.35 NGRR28 pKa = 11.84 KK29 pKa = 8.13 VLKK32 pKa = 10.34 ARR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.22 GRR39 pKa = 11.84 KK40 pKa = 8.88 VLTAA44 pKa = 4.27
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.067
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.676
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.398
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.038
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6817
0
6817
2409860
26
4008
353.5
39.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.346 ± 0.039
0.731 ± 0.01
5.271 ± 0.02
6.868 ± 0.034
4.066 ± 0.022
7.945 ± 0.032
1.872 ± 0.013
5.936 ± 0.025
4.633 ± 0.025
10.196 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.576 ± 0.013
3.468 ± 0.023
4.322 ± 0.018
3.38 ± 0.016
5.988 ± 0.033
6.283 ± 0.026
5.105 ± 0.028
7.104 ± 0.022
1.42 ± 0.014
3.492 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here