Thauera sp. D20

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Thauera; unclassified Thauera

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3318 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T4IBB4|A0A2T4IBB4_9RHOO Alanine racemase OS=Thauera sp. D20 OX=2136175 GN=alr PE=3 SV=1
MM1 pKa = 7.03NAVVEE6 pKa = 4.54TPEE9 pKa = 3.94VLVFTDD15 pKa = 3.66SAANKK20 pKa = 8.85VRR22 pKa = 11.84EE23 pKa = 4.42LIEE26 pKa = 4.47EE27 pKa = 4.28EE28 pKa = 4.33GNPALKK34 pKa = 10.57LRR36 pKa = 11.84VFVTGGGCSGFQYY49 pKa = 10.93GFTFDD54 pKa = 5.12EE55 pKa = 4.54EE56 pKa = 4.44TADD59 pKa = 5.1DD60 pKa = 3.73DD61 pKa = 4.76TAFEE65 pKa = 4.65KK66 pKa = 11.2NGVTLLIDD74 pKa = 3.7PMSYY78 pKa = 10.31QYY80 pKa = 11.58LVGAEE85 pKa = 3.64IDD87 pKa = 3.75YY88 pKa = 10.77TEE90 pKa = 4.43GLEE93 pKa = 4.25GSQFVIRR100 pKa = 11.84NPNATSTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T4IIC3|A0A2T4IIC3_9RHOO MFS transporter OS=Thauera sp. D20 OX=2136175 GN=C8261_02240 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.6RR14 pKa = 11.84THH16 pKa = 5.75GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3318

0

3318

1104624

26

2868

332.9

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.635 ± 0.065

1.017 ± 0.017

5.484 ± 0.034

5.923 ± 0.042

3.496 ± 0.022

8.337 ± 0.041

2.436 ± 0.022

4.431 ± 0.031

2.471 ± 0.032

11.339 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.021

2.37 ± 0.023

4.972 ± 0.033

3.468 ± 0.023

7.918 ± 0.042

4.853 ± 0.029

4.538 ± 0.026

7.479 ± 0.04

1.368 ± 0.019

2.179 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski