Amino acid dipepetide frequency for Thauera sp. D20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.65AlaAla: 21.65 ± 0.242
1.529AlaCys: 1.529 ± 0.042
7.364AlaAsp: 7.364 ± 0.079
8.504AlaGlu: 8.504 ± 0.105
4.166AlaPhe: 4.166 ± 0.067
11.62AlaGly: 11.62 ± 0.131
3.106AlaHis: 3.106 ± 0.063
5.281AlaIle: 5.281 ± 0.078
2.411AlaLys: 2.411 ± 0.064
17.2AlaLeu: 17.2 ± 0.181
3.169AlaMet: 3.169 ± 0.055
2.642AlaAsn: 2.642 ± 0.056
5.998AlaPro: 5.998 ± 0.08
4.978AlaGln: 4.978 ± 0.078
11.119AlaArg: 11.119 ± 0.119
5.889AlaSer: 5.889 ± 0.078
5.765AlaThr: 5.765 ± 0.068
9.714AlaVal: 9.714 ± 0.097
1.866AlaTrp: 1.866 ± 0.048
2.385AlaTyr: 2.385 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
1.427CysAla: 1.427 ± 0.039
0.158CysCys: 0.158 ± 0.012
0.543CysAsp: 0.543 ± 0.024
0.562CysGlu: 0.562 ± 0.023
0.323CysPhe: 0.323 ± 0.019
1.116CysGly: 1.116 ± 0.034
0.33CysHis: 0.33 ± 0.021
0.369CysIle: 0.369 ± 0.018
0.204CysLys: 0.204 ± 0.015
0.946CysLeu: 0.946 ± 0.029
0.21CysMet: 0.21 ± 0.014
0.24CysAsn: 0.24 ± 0.016
0.557CysPro: 0.557 ± 0.024
0.238CysGln: 0.238 ± 0.015
0.774CysArg: 0.774 ± 0.028
0.539CysSer: 0.539 ± 0.022
0.542CysThr: 0.542 ± 0.021
0.723CysVal: 0.723 ± 0.024
0.136CysTrp: 0.136 ± 0.012
0.237CysTyr: 0.237 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
7.358AspAla: 7.358 ± 0.092
0.531AspCys: 0.531 ± 0.024
3.209AspAsp: 3.209 ± 0.07
3.799AspGlu: 3.799 ± 0.057
2.287AspPhe: 2.287 ± 0.046
5.389AspGly: 5.389 ± 0.09
1.273AspHis: 1.273 ± 0.035
2.688AspIle: 2.688 ± 0.056
1.433AspLys: 1.433 ± 0.04
5.42AspLeu: 5.42 ± 0.073
1.137AspMet: 1.137 ± 0.036
1.281AspAsn: 1.281 ± 0.035
2.968AspPro: 2.968 ± 0.044
1.458AspGln: 1.458 ± 0.035
3.502AspArg: 3.502 ± 0.063
2.462AspSer: 2.462 ± 0.048
2.564AspThr: 2.564 ± 0.045
3.529AspVal: 3.529 ± 0.051
0.97AspTrp: 0.97 ± 0.028
1.581AspTyr: 1.581 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
8.194GluAla: 8.194 ± 0.101
0.471GluCys: 0.471 ± 0.021
2.659GluAsp: 2.659 ± 0.051
3.287GluGlu: 3.287 ± 0.065
1.836GluPhe: 1.836 ± 0.047
4.209GluGly: 4.209 ± 0.062
1.624GluHis: 1.624 ± 0.041
3.082GluIle: 3.082 ± 0.057
1.62GluLys: 1.62 ± 0.045
7.078GluLeu: 7.078 ± 0.091
1.41GluMet: 1.41 ± 0.033
1.294GluAsn: 1.294 ± 0.038
2.199GluPro: 2.199 ± 0.052
2.533GluGln: 2.533 ± 0.055
5.986GluArg: 5.986 ± 0.077
2.437GluSer: 2.437 ± 0.052
2.493GluThr: 2.493 ± 0.05
4.896GluVal: 4.896 ± 0.073
0.79GluTrp: 0.79 ± 0.03
1.133GluTyr: 1.133 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
4.524PheAla: 4.524 ± 0.071
0.388PheCys: 0.388 ± 0.023
2.59PheAsp: 2.59 ± 0.048
2.278PheGlu: 2.278 ± 0.046
1.39PhePhe: 1.39 ± 0.041
3.253PheGly: 3.253 ± 0.057
0.82PheHis: 0.82 ± 0.024
1.64PheIle: 1.64 ± 0.039
0.836PheLys: 0.836 ± 0.028
3.115PheLeu: 3.115 ± 0.055
0.739PheMet: 0.739 ± 0.024
1.01PheAsn: 1.01 ± 0.029
1.408PhePro: 1.408 ± 0.037
0.92PheGln: 0.92 ± 0.027
2.276PheArg: 2.276 ± 0.042
2.021PheSer: 2.021 ± 0.038
1.706PheThr: 1.706 ± 0.045
2.747PheVal: 2.747 ± 0.051
0.447PheTrp: 0.447 ± 0.022
0.852PheTyr: 0.852 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
9.322GlyAla: 9.322 ± 0.104
1.014GlyCys: 1.014 ± 0.031
4.38GlyAsp: 4.38 ± 0.089
5.796GlyGlu: 5.796 ± 0.084
3.202GlyPhe: 3.202 ± 0.052
7.319GlyGly: 7.319 ± 0.108
2.054GlyHis: 2.054 ± 0.047
3.906GlyIle: 3.906 ± 0.063
2.697GlyLys: 2.697 ± 0.061
8.84GlyLeu: 8.84 ± 0.1
2.42GlyMet: 2.42 ± 0.056
2.069GlyAsn: 2.069 ± 0.053
2.521GlyPro: 2.521 ± 0.054
2.871GlyGln: 2.871 ± 0.05
6.651GlyArg: 6.651 ± 0.073
4.488GlySer: 4.488 ± 0.076
3.878GlyThr: 3.878 ± 0.061
6.491GlyVal: 6.491 ± 0.082
1.332GlyTrp: 1.332 ± 0.042
2.115GlyTyr: 2.115 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
3.179HisAla: 3.179 ± 0.064
0.318HisCys: 0.318 ± 0.016
1.391HisAsp: 1.391 ± 0.035
1.22HisGlu: 1.22 ± 0.036
0.979HisPhe: 0.979 ± 0.035
2.443HisGly: 2.443 ± 0.053
0.756HisHis: 0.756 ± 0.03
0.985HisIle: 0.985 ± 0.028
0.5HisLys: 0.5 ± 0.019
2.615HisLeu: 2.615 ± 0.054
0.443HisMet: 0.443 ± 0.021
0.549HisAsn: 0.549 ± 0.023
1.667HisPro: 1.667 ± 0.042
0.675HisGln: 0.675 ± 0.023
1.723HisArg: 1.723 ± 0.039
1.088HisSer: 1.088 ± 0.03
1.112HisThr: 1.112 ± 0.03
1.517HisVal: 1.517 ± 0.036
0.407HisTrp: 0.407 ± 0.021
0.789HisTyr: 0.789 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.351IleAla: 6.351 ± 0.078
0.432IleCys: 0.432 ± 0.021
3.431IleAsp: 3.431 ± 0.053
3.267IleGlu: 3.267 ± 0.061
1.308IlePhe: 1.308 ± 0.035
4.281IleGly: 4.281 ± 0.068
1.068IleHis: 1.068 ± 0.028
1.732IleIle: 1.732 ± 0.051
1.159IleLys: 1.159 ± 0.037
3.838IleLeu: 3.838 ± 0.076
0.746IleMet: 0.746 ± 0.025
1.399IleAsn: 1.399 ± 0.036
2.034IlePro: 2.034 ± 0.046
1.028IleGln: 1.028 ± 0.03
3.019IleArg: 3.019 ± 0.059
2.267IleSer: 2.267 ± 0.044
2.26IleThr: 2.26 ± 0.047
3.362IleVal: 3.362 ± 0.063
0.398IleTrp: 0.398 ± 0.021
0.926IleTyr: 0.926 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.15LysAla: 3.15 ± 0.062
0.165LysCys: 0.165 ± 0.013
1.221LysAsp: 1.221 ± 0.038
1.236LysGlu: 1.236 ± 0.04
0.68LysPhe: 0.68 ± 0.027
1.88LysGly: 1.88 ± 0.047
0.575LysHis: 0.575 ± 0.025
1.214LysIle: 1.214 ± 0.044
0.925LysLys: 0.925 ± 0.036
2.8LysLeu: 2.8 ± 0.06
0.573LysMet: 0.573 ± 0.027
0.663LysAsn: 0.663 ± 0.024
1.445LysPro: 1.445 ± 0.039
0.898LysGln: 0.898 ± 0.031
1.86LysArg: 1.86 ± 0.048
1.287LysSer: 1.287 ± 0.037
1.224LysThr: 1.224 ± 0.038
2.095LysVal: 2.095 ± 0.053
0.277LysTrp: 0.277 ± 0.014
0.544LysTyr: 0.544 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
17.105LeuAla: 17.105 ± 0.19
1.195LeuCys: 1.195 ± 0.034
6.417LeuAsp: 6.417 ± 0.07
5.962LeuGlu: 5.962 ± 0.08
3.782LeuPhe: 3.782 ± 0.067
9.296LeuGly: 9.296 ± 0.105
2.514LeuHis: 2.514 ± 0.054
4.771LeuIle: 4.771 ± 0.073
2.95LeuLys: 2.95 ± 0.059
12.468LeuLeu: 12.468 ± 0.169
2.208LeuMet: 2.208 ± 0.047
2.554LeuAsn: 2.554 ± 0.054
6.541LeuPro: 6.541 ± 0.075
4.093LeuGln: 4.093 ± 0.061
9.369LeuArg: 9.369 ± 0.101
6.003LeuSer: 6.003 ± 0.088
5.061LeuThr: 5.061 ± 0.075
7.6LeuVal: 7.6 ± 0.1
1.338LeuTrp: 1.338 ± 0.049
2.167LeuTyr: 2.167 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.759MetAla: 2.759 ± 0.053
0.169MetCys: 0.169 ± 0.013
1.151MetAsp: 1.151 ± 0.029
1.139MetGlu: 1.139 ± 0.034
0.59MetPhe: 0.59 ± 0.021
1.588MetGly: 1.588 ± 0.035
0.514MetHis: 0.514 ± 0.023
0.987MetIle: 0.987 ± 0.034
0.901MetLys: 0.901 ± 0.03
2.664MetLeu: 2.664 ± 0.049
0.572MetMet: 0.572 ± 0.022
0.796MetAsn: 0.796 ± 0.027
1.348MetPro: 1.348 ± 0.034
0.894MetGln: 0.894 ± 0.027
1.769MetArg: 1.769 ± 0.037
1.571MetSer: 1.571 ± 0.036
1.235MetThr: 1.235 ± 0.033
1.621MetVal: 1.621 ± 0.041
0.214MetTrp: 0.214 ± 0.015
0.373MetTyr: 0.373 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.049AsnAla: 3.049 ± 0.056
0.278AsnCys: 0.278 ± 0.018
1.22AsnAsp: 1.22 ± 0.034
1.171AsnGlu: 1.171 ± 0.034
0.786AsnPhe: 0.786 ± 0.029
1.954AsnGly: 1.954 ± 0.046
0.513AsnHis: 0.513 ± 0.021
1.22AsnIle: 1.22 ± 0.034
0.61AsnLys: 0.61 ± 0.024
2.631AsnLeu: 2.631 ± 0.052
0.557AsnMet: 0.557 ± 0.024
0.632AsnAsn: 0.632 ± 0.027
1.701AsnPro: 1.701 ± 0.045
0.745AsnGln: 0.745 ± 0.024
1.839AsnArg: 1.839 ± 0.042
0.954AsnSer: 0.954 ± 0.027
1.219AsnThr: 1.219 ± 0.038
1.66AsnVal: 1.66 ± 0.036
0.361AsnTrp: 0.361 ± 0.019
0.601AsnTyr: 0.601 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
7.883ProAla: 7.883 ± 0.096
0.474ProCys: 0.474 ± 0.024
2.783ProAsp: 2.783 ± 0.054
3.316ProGlu: 3.316 ± 0.063
1.797ProPhe: 1.797 ± 0.036
4.348ProGly: 4.348 ± 0.072
1.068ProHis: 1.068 ± 0.034
1.639ProIle: 1.639 ± 0.038
0.976ProLys: 0.976 ± 0.03
5.356ProLeu: 5.356 ± 0.081
1.027ProMet: 1.027 ± 0.028
1.004ProAsn: 1.004 ± 0.034
3.068ProPro: 3.068 ± 0.072
2.08ProGln: 2.08 ± 0.047
3.129ProArg: 3.129 ± 0.056
2.023ProSer: 2.023 ± 0.042
2.037ProThr: 2.037 ± 0.039
3.91ProVal: 3.91 ± 0.057
0.73ProTrp: 0.73 ± 0.024
1.074ProTyr: 1.074 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
4.902GlnAla: 4.902 ± 0.074
0.301GlnCys: 0.301 ± 0.015
1.389GlnAsp: 1.389 ± 0.035
1.584GlnGlu: 1.584 ± 0.044
1.085GlnPhe: 1.085 ± 0.032
2.652GlnGly: 2.652 ± 0.039
0.961GlnHis: 0.961 ± 0.033
1.632GlnIle: 1.632 ± 0.042
0.826GlnLys: 0.826 ± 0.033
3.866GlnLeu: 3.866 ± 0.067
0.86GlnMet: 0.86 ± 0.031
0.654GlnAsn: 0.654 ± 0.026
2.027GlnPro: 2.027 ± 0.047
1.59GlnGln: 1.59 ± 0.041
3.422GlnArg: 3.422 ± 0.061
1.519GlnSer: 1.519 ± 0.042
1.487GlnThr: 1.487 ± 0.038
2.699GlnVal: 2.699 ± 0.046
0.576GlnTrp: 0.576 ± 0.021
0.651GlnTyr: 0.651 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
9.345ArgAla: 9.345 ± 0.122
0.739ArgCys: 0.739 ± 0.028
4.067ArgAsp: 4.067 ± 0.067
5.029ArgGlu: 5.029 ± 0.074
3.21ArgPhe: 3.21 ± 0.06
5.216ArgGly: 5.216 ± 0.069
2.33ArgHis: 2.33 ± 0.048
4.244ArgIle: 4.244 ± 0.065
1.993ArgLys: 1.993 ± 0.048
9.678ArgLeu: 9.678 ± 0.094
2.116ArgMet: 2.116 ± 0.046
2.065ArgAsn: 2.065 ± 0.04
3.568ArgPro: 3.568 ± 0.058
3.11ArgGln: 3.11 ± 0.057
6.655ArgArg: 6.655 ± 0.091
3.83ArgSer: 3.83 ± 0.066
3.14ArgThr: 3.14 ± 0.061
5.577ArgVal: 5.577 ± 0.071
1.182ArgTrp: 1.182 ± 0.032
2.084ArgTyr: 2.084 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
7.097SerAla: 7.097 ± 0.09
0.484SerCys: 0.484 ± 0.022
2.28SerAsp: 2.28 ± 0.051
2.511SerGlu: 2.511 ± 0.05
1.97SerPhe: 1.97 ± 0.042
5.018SerGly: 5.018 ± 0.073
1.085SerHis: 1.085 ± 0.031
2.194SerIle: 2.194 ± 0.043
1.064SerLys: 1.064 ± 0.033
5.023SerLeu: 5.023 ± 0.068
1.114SerMet: 1.114 ± 0.033
1.155SerAsn: 1.155 ± 0.037
2.417SerPro: 2.417 ± 0.046
1.377SerGln: 1.377 ± 0.035
3.582SerArg: 3.582 ± 0.06
2.466SerSer: 2.466 ± 0.059
2.492SerThr: 2.492 ± 0.058
3.443SerVal: 3.443 ± 0.055
0.671SerTrp: 0.671 ± 0.027
1.089SerTyr: 1.089 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
5.728ThrAla: 5.728 ± 0.077
0.382ThrCys: 0.382 ± 0.019
2.155ThrAsp: 2.155 ± 0.047
2.267ThrGlu: 2.267 ± 0.045
1.45ThrPhe: 1.45 ± 0.037
3.742ThrGly: 3.742 ± 0.065
1.098ThrHis: 1.098 ± 0.03
1.843ThrIle: 1.843 ± 0.044
0.807ThrLys: 0.807 ± 0.031
6.328ThrLeu: 6.328 ± 0.085
0.885ThrMet: 0.885 ± 0.03
0.967ThrAsn: 0.967 ± 0.034
3.039ThrPro: 3.039 ± 0.056
1.392ThrGln: 1.392 ± 0.034
3.427ThrArg: 3.427 ± 0.055
1.942ThrSer: 1.942 ± 0.048
2.3ThrThr: 2.3 ± 0.058
4.121ThrVal: 4.121 ± 0.064
0.55ThrTrp: 0.55 ± 0.028
0.961ThrTyr: 0.961 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
9.515ValAla: 9.515 ± 0.102
0.749ValCys: 0.749 ± 0.026
4.776ValAsp: 4.776 ± 0.065
4.593ValGlu: 4.593 ± 0.076
2.764ValPhe: 2.764 ± 0.053
5.291ValGly: 5.291 ± 0.067
1.782ValHis: 1.782 ± 0.035
3.56ValIle: 3.56 ± 0.07
1.927ValLys: 1.927 ± 0.054
8.822ValLeu: 8.822 ± 0.098
1.851ValMet: 1.851 ± 0.041
1.81ValAsn: 1.81 ± 0.044
3.436ValPro: 3.436 ± 0.056
2.145ValGln: 2.145 ± 0.048
5.721ValArg: 5.721 ± 0.076
4.059ValSer: 4.059 ± 0.055
3.279ValThr: 3.279 ± 0.055
6.499ValVal: 6.499 ± 0.092
0.836ValTrp: 0.836 ± 0.034
1.369ValTyr: 1.369 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.289TrpAla: 1.289 ± 0.038
0.149TrpCys: 0.149 ± 0.011
0.581TrpAsp: 0.581 ± 0.025
0.627TrpGlu: 0.627 ± 0.024
0.492TrpPhe: 0.492 ± 0.021
0.83TrpGly: 0.83 ± 0.031
0.337TrpHis: 0.337 ± 0.018
0.556TrpIle: 0.556 ± 0.023
0.355TrpLys: 0.355 ± 0.017
2.139TrpLeu: 2.139 ± 0.051
0.382TrpMet: 0.382 ± 0.019
0.39TrpAsn: 0.39 ± 0.018
0.652TrpPro: 0.652 ± 0.025
0.853TrpGln: 0.853 ± 0.027
1.31TrpArg: 1.31 ± 0.041
0.649TrpSer: 0.649 ± 0.027
0.549TrpThr: 0.549 ± 0.026
1.015TrpVal: 1.015 ± 0.028
0.253TrpTrp: 0.253 ± 0.015
0.269TrpTyr: 0.269 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.527TyrAla: 2.527 ± 0.05
0.249TyrCys: 0.249 ± 0.013
1.231TyrAsp: 1.231 ± 0.033
1.081TyrGlu: 1.081 ± 0.033
0.863TyrPhe: 0.863 ± 0.029
1.917TyrGly: 1.917 ± 0.044
0.549TyrHis: 0.549 ± 0.022
0.763TyrIle: 0.763 ± 0.025
0.52TyrLys: 0.52 ± 0.024
2.566TyrLeu: 2.566 ± 0.047
0.434TyrMet: 0.434 ± 0.02
0.53TyrAsn: 0.53 ± 0.025
1.129TyrPro: 1.129 ± 0.036
0.805TyrGln: 0.805 ± 0.029
2.047TyrArg: 2.047 ± 0.047
1.039TyrSer: 1.039 ± 0.032
1.041TyrThr: 1.041 ± 0.032
1.568TyrVal: 1.568 ± 0.036
0.342TyrTrp: 0.342 ± 0.019
0.593TyrTyr: 0.593 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3318 proteins (1104624 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski