Tibeticola sediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Tibeticola

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2531 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N4UH35|A0A3N4UH35_9BURK Uncharacterized protein OS=Tibeticola sediminis OX=1917811 GN=EDC62_2296 PE=4 SV=1
MM1 pKa = 7.63SEE3 pKa = 4.3VAEE6 pKa = 4.39STAPAVAAPQTPPDD20 pKa = 4.84PIIFTDD26 pKa = 3.67AAAAKK31 pKa = 9.92VADD34 pKa = 5.47LIAEE38 pKa = 4.31EE39 pKa = 4.82GNPDD43 pKa = 2.99LKK45 pKa = 11.13LRR47 pKa = 11.84VFVQGGGCSGFQYY60 pKa = 10.94GFTFDD65 pKa = 5.37EE66 pKa = 5.53IINDD70 pKa = 4.31DD71 pKa = 4.02DD72 pKa = 4.23TVMTKK77 pKa = 10.7NGVTLLIDD85 pKa = 3.64AMSYY89 pKa = 10.32QYY91 pKa = 11.5LVGAEE96 pKa = 3.79IDD98 pKa = 3.91YY99 pKa = 11.36KK100 pKa = 11.19EE101 pKa = 4.67DD102 pKa = 3.23LQGAQFVIRR111 pKa = 11.84NPNATTTCGCGSSFSTSS128 pKa = 2.74

Molecular weight: 13.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N4UHW2|A0A3N4UHW2_9BURK TFIIB-like protein OS=Tibeticola sediminis OX=1917811 GN=EDC62_1955 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIKK32 pKa = 10.36ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2531

0

2531

831099

29

1950

328.4

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.753 ± 0.074

0.915 ± 0.015

4.97 ± 0.032

5.82 ± 0.043

3.341 ± 0.032

8.129 ± 0.038

2.25 ± 0.024

4.136 ± 0.035

2.82 ± 0.039

11.068 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.022

2.212 ± 0.029

5.702 ± 0.04

3.834 ± 0.034

7.855 ± 0.051

4.846 ± 0.033

4.8 ± 0.036

7.553 ± 0.04

1.633 ± 0.024

2.122 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski