Nocardioides sp. GY 10127

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3765 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4T0USX8|A0A4T0USX8_9ACTN ATP-dependent Clp protease proteolytic subunit OS=Nocardioides sp. GY 10127 OX=2569762 GN=E8D37_11570 PE=3 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84SRR6 pKa = 11.84VAASLGVIALATTLLAACGSEE27 pKa = 4.13SSEE30 pKa = 3.84DD31 pKa = 3.64TAAEE35 pKa = 3.93TAAAGATDD43 pKa = 4.96LAAAGCPATIVIQTDD58 pKa = 3.34WNPEE62 pKa = 3.83AEE64 pKa = 4.33HH65 pKa = 5.57GHH67 pKa = 6.45LYY69 pKa = 11.17EE70 pKa = 4.69MFGDD74 pKa = 4.52DD75 pKa = 4.46ATIDD79 pKa = 3.78ADD81 pKa = 3.78NKK83 pKa = 10.24SVSGTLYY90 pKa = 11.07DD91 pKa = 3.69NGEE94 pKa = 4.19STGVKK99 pKa = 10.16LEE101 pKa = 3.86VRR103 pKa = 11.84AGGPAIGYY111 pKa = 7.08STVSAQMYY119 pKa = 7.28TDD121 pKa = 3.6KK122 pKa = 10.75SITMGYY128 pKa = 7.91VTTDD132 pKa = 3.29DD133 pKa = 4.52QIASSATTPTKK144 pKa = 10.89SFFAPLDD151 pKa = 3.85LSPLMVMWDD160 pKa = 3.28PTYY163 pKa = 10.92YY164 pKa = 10.16PDD166 pKa = 3.57VTGIDD171 pKa = 3.95DD172 pKa = 5.31LKK174 pKa = 11.32DD175 pKa = 3.64ALDD178 pKa = 3.61SSGGVWRR185 pKa = 11.84YY186 pKa = 8.08FAGSAYY192 pKa = 9.62MDD194 pKa = 3.81YY195 pKa = 10.89LEE197 pKa = 4.44SAGYY201 pKa = 9.53VSKK204 pKa = 11.33DD205 pKa = 3.64SMDD208 pKa = 3.14SSYY211 pKa = 11.7DD212 pKa = 3.29GTPANFVAAGGKK224 pKa = 9.36DD225 pKa = 3.61VQQGFASAEE234 pKa = 3.91PYY236 pKa = 10.11QYY238 pKa = 11.17EE239 pKa = 4.38NEE241 pKa = 3.99VKK243 pKa = 10.32AWGKK247 pKa = 10.18KK248 pKa = 7.63VDD250 pKa = 3.67YY251 pKa = 11.0ALISDD256 pKa = 4.49TGWDD260 pKa = 4.08PYY262 pKa = 10.94QSSLAVRR269 pKa = 11.84SADD272 pKa = 3.86FDD274 pKa = 4.38DD275 pKa = 6.81LSDD278 pKa = 4.29CLAALTPVLQRR289 pKa = 11.84AEE291 pKa = 4.16VNYY294 pKa = 10.16FADD297 pKa = 3.78PTDD300 pKa = 3.7ANNLVLEE307 pKa = 4.67AVQEE311 pKa = 4.43YY312 pKa = 7.96NTGWTYY318 pKa = 10.13SQGMADD324 pKa = 3.69YY325 pKa = 10.66SVEE328 pKa = 4.02TMLGDD333 pKa = 3.72KK334 pKa = 10.51LVSNGDD340 pKa = 2.98NDD342 pKa = 4.39YY343 pKa = 11.28IGDD346 pKa = 3.87FDD348 pKa = 6.14DD349 pKa = 5.76DD350 pKa = 4.22RR351 pKa = 11.84MSAFFDD357 pKa = 3.81KK358 pKa = 10.39ATKK361 pKa = 9.65TYY363 pKa = 10.66SALGTDD369 pKa = 3.6MASGLKK375 pKa = 10.6VSDD378 pKa = 4.57LYY380 pKa = 11.27TNEE383 pKa = 5.2FIDD386 pKa = 5.43DD387 pKa = 4.38SIGLSNN393 pKa = 4.32

Molecular weight: 42.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4T0UN17|A0A4T0UN17_9ACTN GntP family transporter OS=Nocardioides sp. GY 10127 OX=2569762 GN=E8D37_15215 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3765

0

3765

1280276

33

2019

340.0

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.812 ± 0.065

0.706 ± 0.011

6.379 ± 0.034

5.698 ± 0.038

2.494 ± 0.024

9.539 ± 0.041

2.19 ± 0.021

2.641 ± 0.032

1.676 ± 0.031

10.745 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.666 ± 0.016

1.496 ± 0.02

5.697 ± 0.035

2.614 ± 0.021

7.623 ± 0.043

5.482 ± 0.034

6.352 ± 0.04

9.841 ± 0.042

1.539 ± 0.018

1.811 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski