Amino acid dipepetide frequency for Nocardioides sp. GY 10127

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.431AlaAla: 21.431 ± 0.219	1.081AlaCys: 1.081 ± 0.028	8.961AlaAsp: 8.961 ± 0.102	8.647AlaGlu: 8.647 ± 0.135	3.304AlaPhe: 3.304 ± 0.064	13.781AlaGly: 13.781 ± 0.127	2.799AlaHis: 2.799 ± 0.05	3.397AlaIle: 3.397 ± 0.061	2.148AlaLys: 2.148 ± 0.053	14.587AlaLeu: 14.587 ± 0.146	2.671AlaMet: 2.671 ± 0.046	1.673AlaAsn: 1.673 ± 0.04	6.813AlaPro: 6.813 ± 0.107	3.755AlaGln: 3.755 ± 0.068	10.37AlaArg: 10.37 ± 0.118	7.187AlaSer: 7.187 ± 0.086	8.078AlaThr: 8.078 ± 0.112	12.811AlaVal: 12.811 ± 0.117	2.24AlaTrp: 2.24 ± 0.044	2.384AlaTyr: 2.384 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.876CysAla: 0.876 ± 0.027	0.068CysCys: 0.068 ± 0.007	0.436CysAsp: 0.436 ± 0.021	0.355CysGlu: 0.355 ± 0.015	0.21CysPhe: 0.21 ± 0.013	0.797CysGly: 0.797 ± 0.025	0.171CysHis: 0.171 ± 0.011	0.155CysIle: 0.155 ± 0.011	0.099CysLys: 0.099 ± 0.009	0.73CysLeu: 0.73 ± 0.025	0.101CysMet: 0.101 ± 0.008	0.102CysAsn: 0.102 ± 0.008	0.44CysPro: 0.44 ± 0.019	0.169CysGln: 0.169 ± 0.012	0.508CysArg: 0.508 ± 0.021	0.478CysSer: 0.478 ± 0.02	0.491CysThr: 0.491 ± 0.024	0.62CysVal: 0.62 ± 0.021	0.123CysTrp: 0.123 ± 0.009	0.132CysTyr: 0.132 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	9.006AspAla: 9.006 ± 0.088	0.325AspCys: 0.325 ± 0.016	4.516AspAsp: 4.516 ± 0.068	4.186AspGlu: 4.186 ± 0.056	1.417AspPhe: 1.417 ± 0.036	6.755AspGly: 6.755 ± 0.088	1.376AspHis: 1.376 ± 0.038	1.326AspIle: 1.326 ± 0.039	0.881AspLys: 0.881 ± 0.027	7.669AspLeu: 7.669 ± 0.09	0.772AspMet: 0.772 ± 0.023	0.78AspAsn: 0.78 ± 0.032	4.676AspPro: 4.676 ± 0.071	1.543AspGln: 1.543 ± 0.032	4.213AspArg: 4.213 ± 0.063	2.62AspSer: 2.62 ± 0.054	3.081AspThr: 3.081 ± 0.061	6.609AspVal: 6.609 ± 0.083	0.939AspTrp: 0.939 ± 0.029	1.103AspTyr: 1.103 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	7.923GluAla: 7.923 ± 0.119	0.326GluCys: 0.326 ± 0.019	3.417GluAsp: 3.417 ± 0.054	3.481GluGlu: 3.481 ± 0.062	1.083GluPhe: 1.083 ± 0.028	4.479GluGly: 4.479 ± 0.073	1.679GluHis: 1.679 ± 0.043	2.025GluIle: 2.025 ± 0.051	1.323GluLys: 1.323 ± 0.039	5.778GluLeu: 5.778 ± 0.078	0.888GluMet: 0.888 ± 0.032	0.964GluAsn: 0.964 ± 0.027	3.348GluPro: 3.348 ± 0.057	2.331GluGln: 2.331 ± 0.05	5.268GluArg: 5.268 ± 0.097	2.496GluSer: 2.496 ± 0.043	2.949GluThr: 2.949 ± 0.053	5.618GluVal: 5.618 ± 0.064	0.733GluTrp: 0.733 ± 0.024	0.868GluTyr: 0.868 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	3.298PheAla: 3.298 ± 0.06	0.251PheCys: 0.251 ± 0.015	1.767PheAsp: 1.767 ± 0.044	1.36PheGlu: 1.36 ± 0.033	0.788PhePhe: 0.788 ± 0.024	2.711PheGly: 2.711 ± 0.045	0.52PheHis: 0.52 ± 0.019	0.601PheIle: 0.601 ± 0.026	0.376PheLys: 0.376 ± 0.019	2.499PheLeu: 2.499 ± 0.052	0.361PheMet: 0.361 ± 0.017	0.505PheAsn: 0.505 ± 0.019	1.164PhePro: 1.164 ± 0.031	0.519PheGln: 0.519 ± 0.02	1.536PheArg: 1.536 ± 0.041	1.519PheSer: 1.519 ± 0.036	1.825PheThr: 1.825 ± 0.039	2.399PheVal: 2.399 ± 0.049	0.396PheTrp: 0.396 ± 0.018	0.549PheTyr: 0.549 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	11.23GlyAla: 11.23 ± 0.118	0.853GlyCys: 0.853 ± 0.029	5.367GlyAsp: 5.367 ± 0.077	5.238GlyGlu: 5.238 ± 0.075	2.785GlyPhe: 2.785 ± 0.049	8.53GlyGly: 8.53 ± 0.102	2.245GlyHis: 2.245 ± 0.053	2.996GlyIle: 2.996 ± 0.056	1.814GlyLys: 1.814 ± 0.039	10.709GlyLeu: 10.709 ± 0.096	1.924GlyMet: 1.924 ± 0.042	1.504GlyAsn: 1.504 ± 0.044	4.708GlyPro: 4.708 ± 0.072	2.489GlyGln: 2.489 ± 0.048	7.673GlyArg: 7.673 ± 0.09	6.119GlySer: 6.119 ± 0.082	6.606GlyThr: 6.606 ± 0.1	8.626GlyVal: 8.626 ± 0.087	1.846GlyTrp: 1.846 ± 0.036	2.122GlyTyr: 2.122 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.768HisAla: 2.768 ± 0.055	0.163HisCys: 0.163 ± 0.011	1.395HisAsp: 1.395 ± 0.035	1.324HisGlu: 1.324 ± 0.029	0.491HisPhe: 0.491 ± 0.02	2.314HisGly: 2.314 ± 0.039	0.649HisHis: 0.649 ± 0.024	0.357HisIle: 0.357 ± 0.017	0.232HisLys: 0.232 ± 0.015	2.579HisLeu: 2.579 ± 0.052	0.266HisMet: 0.266 ± 0.013	0.262HisAsn: 0.262 ± 0.013	1.743HisPro: 1.743 ± 0.039	0.605HisGln: 0.605 ± 0.018	1.982HisArg: 1.982 ± 0.049	0.787HisSer: 0.787 ± 0.024	1.056HisThr: 1.056 ± 0.033	2.228HisVal: 2.228 ± 0.038	0.312HisTrp: 0.312 ± 0.015	0.378HisTyr: 0.378 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	3.819IleAla: 3.819 ± 0.065	0.226IleCys: 0.226 ± 0.013	2.06IleAsp: 2.06 ± 0.04	1.805IleGlu: 1.805 ± 0.049	0.636IlePhe: 0.636 ± 0.029	2.986IleGly: 2.986 ± 0.066	0.457IleHis: 0.457 ± 0.017	0.752IleIle: 0.752 ± 0.034	0.577IleLys: 0.577 ± 0.026	2.048IleLeu: 2.048 ± 0.054	0.376IleMet: 0.376 ± 0.019	0.576IleAsn: 0.576 ± 0.02	1.261IlePro: 1.261 ± 0.035	0.582IleGln: 0.582 ± 0.024	1.539IleArg: 1.539 ± 0.042	1.537IleSer: 1.537 ± 0.039	1.864IleThr: 1.864 ± 0.045	2.543IleVal: 2.543 ± 0.054	0.301IleTrp: 0.301 ± 0.015	0.46IleTyr: 0.46 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	2.276LysAla: 2.276 ± 0.054	0.066LysCys: 0.066 ± 0.006	1.019LysAsp: 1.019 ± 0.028	0.872LysGlu: 0.872 ± 0.03	0.367LysPhe: 0.367 ± 0.018	1.398LysGly: 1.398 ± 0.037	0.379LysHis: 0.379 ± 0.019	0.686LysIle: 0.686 ± 0.029	0.725LysLys: 0.725 ± 0.034	1.433LysLeu: 1.433 ± 0.039	0.324LysMet: 0.324 ± 0.017	0.398LysAsn: 0.398 ± 0.018	0.958LysPro: 0.958 ± 0.031	0.583LysGln: 0.583 ± 0.024	1.207LysArg: 1.207 ± 0.035	0.876LysSer: 0.876 ± 0.032	1.004LysThr: 1.004 ± 0.034	1.668LysVal: 1.668 ± 0.038	0.167LysTrp: 0.167 ± 0.012	0.352LysTyr: 0.352 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	16.609LeuAla: 16.609 ± 0.156	0.672LeuCys: 0.672 ± 0.023	7.405LeuAsp: 7.405 ± 0.083	5.845LeuGlu: 5.845 ± 0.089	2.27LeuPhe: 2.27 ± 0.047	10.561LeuGly: 10.561 ± 0.108	2.149LeuHis: 2.149 ± 0.042	2.086LeuIle: 2.086 ± 0.056	1.461LeuLys: 1.461 ± 0.036	12.011LeuLeu: 12.011 ± 0.146	1.505LeuMet: 1.505 ± 0.037	1.415LeuAsn: 1.415 ± 0.037	6.31LeuPro: 6.31 ± 0.075	2.153LeuGln: 2.153 ± 0.042	8.142LeuArg: 8.142 ± 0.091	5.418LeuSer: 5.418 ± 0.076	6.909LeuThr: 6.909 ± 0.068	11.866LeuVal: 11.866 ± 0.127	1.168LeuTrp: 1.168 ± 0.033	1.493LeuTyr: 1.493 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.164MetAla: 2.164 ± 0.039	0.113MetCys: 0.113 ± 0.009	0.852MetAsp: 0.852 ± 0.027	0.69MetGlu: 0.69 ± 0.024	0.414MetPhe: 0.414 ± 0.02	1.279MetGly: 1.279 ± 0.033	0.316MetHis: 0.316 ± 0.016	0.569MetIle: 0.569 ± 0.021	0.389MetLys: 0.389 ± 0.017	1.758MetLeu: 1.758 ± 0.04	0.298MetMet: 0.298 ± 0.017	0.322MetAsn: 0.322 ± 0.015	1.086MetPro: 1.086 ± 0.03	0.413MetGln: 0.413 ± 0.017	1.226MetArg: 1.226 ± 0.034	1.429MetSer: 1.429 ± 0.031	1.557MetThr: 1.557 ± 0.034	1.369MetVal: 1.369 ± 0.037	0.172MetTrp: 0.172 ± 0.012	0.244MetTyr: 0.244 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	1.988AsnAla: 1.988 ± 0.046	0.104AsnCys: 0.104 ± 0.01	0.904AsnAsp: 0.904 ± 0.025	0.716AsnGlu: 0.716 ± 0.025	0.408AsnPhe: 0.408 ± 0.019	1.49AsnGly: 1.49 ± 0.047	0.32AsnHis: 0.32 ± 0.016	0.478AsnIle: 0.478 ± 0.021	0.302AsnLys: 0.302 ± 0.016	1.606AsnLeu: 1.606 ± 0.038	0.236AsnMet: 0.236 ± 0.012	0.319AsnAsn: 0.319 ± 0.016	1.186AsnPro: 1.186 ± 0.032	0.435AsnGln: 0.435 ± 0.019	1.032AsnArg: 1.032 ± 0.032	0.665AsnSer: 0.665 ± 0.026	0.874AsnThr: 0.874 ± 0.032	1.377AsnVal: 1.377 ± 0.037	0.185AsnTrp: 0.185 ± 0.013	0.332AsnTyr: 0.332 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	8.272ProAla: 8.272 ± 0.103	0.303ProCys: 0.303 ± 0.015	4.464ProAsp: 4.464 ± 0.066	3.771ProGlu: 3.771 ± 0.06	1.463ProPhe: 1.463 ± 0.034	6.144ProGly: 6.144 ± 0.092	1.295ProHis: 1.295 ± 0.033	1.214ProIle: 1.214 ± 0.034	0.718ProLys: 0.718 ± 0.023	5.335ProLeu: 5.335 ± 0.061	0.821ProMet: 0.821 ± 0.025	0.695ProAsn: 0.695 ± 0.023	2.643ProPro: 2.643 ± 0.056	1.408ProGln: 1.408 ± 0.031	3.872ProArg: 3.872 ± 0.064	3.283ProSer: 3.283 ± 0.06	3.729ProThr: 3.729 ± 0.065	5.589ProVal: 5.589 ± 0.083	0.945ProTrp: 0.945 ± 0.031	1.004ProTyr: 1.004 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.72GlnAla: 3.72 ± 0.064	0.144GlnCys: 0.144 ± 0.011	1.329GlnAsp: 1.329 ± 0.029	1.224GlnGlu: 1.224 ± 0.031	0.611GlnPhe: 0.611 ± 0.024	2.182GlnGly: 2.182 ± 0.046	0.663GlnHis: 0.663 ± 0.025	0.819GlnIle: 0.819 ± 0.027	0.481GlnLys: 0.481 ± 0.021	2.854GlnLeu: 2.854 ± 0.053	0.493GlnMet: 0.493 ± 0.018	0.414GlnAsn: 0.414 ± 0.017	1.617GlnPro: 1.617 ± 0.04	1.054GlnGln: 1.054 ± 0.038	2.329GlnArg: 2.329 ± 0.045	1.07GlnSer: 1.07 ± 0.032	1.369GlnThr: 1.369 ± 0.03	2.945GlnVal: 2.945 ± 0.044	0.371GlnTrp: 0.371 ± 0.018	0.451GlnTyr: 0.451 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	9.759ArgAla: 9.759 ± 0.12	0.512ArgCys: 0.512 ± 0.019	4.112ArgAsp: 4.112 ± 0.061	4.312ArgGlu: 4.312 ± 0.069	2.071ArgPhe: 2.071 ± 0.044	5.96ArgGly: 5.96 ± 0.081	1.82ArgHis: 1.82 ± 0.042	2.23ArgIle: 2.23 ± 0.041	1.248ArgLys: 1.248 ± 0.04	8.593ArgLeu: 8.593 ± 0.086	1.547ArgMet: 1.547 ± 0.034	1.053ArgAsn: 1.053 ± 0.029	4.679ArgPro: 4.679 ± 0.071	1.938ArgGln: 1.938 ± 0.044	7.547ArgArg: 7.547 ± 0.11	4.12ArgSer: 4.12 ± 0.064	5.071ArgThr: 5.071 ± 0.067	6.834ArgVal: 6.834 ± 0.083	1.382ArgTrp: 1.382 ± 0.035	1.442ArgTyr: 1.442 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	7.191SerAla: 7.191 ± 0.091	0.387SerCys: 0.387 ± 0.019	3.202SerAsp: 3.202 ± 0.066	2.48SerGlu: 2.48 ± 0.053	1.62SerPhe: 1.62 ± 0.039	6.1SerGly: 6.1 ± 0.087	1.009SerHis: 1.009 ± 0.031	1.548SerIle: 1.548 ± 0.041	0.855SerLys: 0.855 ± 0.024	5.454SerLeu: 5.454 ± 0.064	1.119SerMet: 1.119 ± 0.032	0.844SerAsn: 0.844 ± 0.029	3.087SerPro: 3.087 ± 0.053	1.387SerGln: 1.387 ± 0.035	3.681SerArg: 3.681 ± 0.054	3.944SerSer: 3.944 ± 0.101	3.999SerThr: 3.999 ± 0.076	4.701SerVal: 4.701 ± 0.067	1.022SerTrp: 1.022 ± 0.026	1.19SerTyr: 1.19 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	8.251ThrAla: 8.251 ± 0.108	0.469ThrCys: 0.469 ± 0.02	4.078ThrAsp: 4.078 ± 0.062	3.039ThrGlu: 3.039 ± 0.048	1.757ThrPhe: 1.757 ± 0.035	6.571ThrGly: 6.571 ± 0.075	1.283ThrHis: 1.283 ± 0.033	1.914ThrIle: 1.914 ± 0.046	0.984ThrLys: 0.984 ± 0.035	6.217ThrLeu: 6.217 ± 0.08	1.011ThrMet: 1.011 ± 0.029	0.944ThrAsn: 0.944 ± 0.028	4.005ThrPro: 4.005 ± 0.07	1.573ThrGln: 1.573 ± 0.035	4.063ThrArg: 4.063 ± 0.059	4.086ThrSer: 4.086 ± 0.076	4.542ThrThr: 4.542 ± 0.082	6.131ThrVal: 6.131 ± 0.09	1.194ThrTrp: 1.194 ± 0.04	1.413ThrTyr: 1.413 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	13.486ValAla: 13.486 ± 0.117	0.691ValCys: 0.691 ± 0.022	6.342ValAsp: 6.342 ± 0.08	5.889ValGlu: 5.889 ± 0.078	2.167ValPhe: 2.167 ± 0.05	8.388ValGly: 8.388 ± 0.102	2.093ValHis: 2.093 ± 0.045	2.452ValIle: 2.452 ± 0.052	1.529ValLys: 1.529 ± 0.039	11.682ValLeu: 11.682 ± 0.13	1.484ValMet: 1.484 ± 0.036	1.536ValAsn: 1.536 ± 0.037	5.55ValPro: 5.55 ± 0.071	2.196ValGln: 2.196 ± 0.045	7.285ValArg: 7.285 ± 0.091	5.183ValSer: 5.183 ± 0.074	6.396ValThr: 6.396 ± 0.088	11.379ValVal: 11.379 ± 0.133	1.221ValTrp: 1.221 ± 0.03	1.46ValTyr: 1.46 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.759TrpAla: 1.759 ± 0.039	0.161TrpCys: 0.161 ± 0.012	0.828TrpAsp: 0.828 ± 0.029	0.737TrpGlu: 0.737 ± 0.027	0.536TrpPhe: 0.536 ± 0.022	1.179TrpGly: 1.179 ± 0.03	0.356TrpHis: 0.356 ± 0.017	0.448TrpIle: 0.448 ± 0.02	0.296TrpLys: 0.296 ± 0.016	1.851TrpLeu: 1.851 ± 0.038	0.273TrpMet: 0.273 ± 0.015	0.301TrpAsn: 0.301 ± 0.017	0.75TrpPro: 0.75 ± 0.024	0.505TrpGln: 0.505 ± 0.019	1.294TrpArg: 1.294 ± 0.032	1.029TrpSer: 1.029 ± 0.03	1.069TrpThr: 1.069 ± 0.034	1.32TrpVal: 1.32 ± 0.036	0.392TrpTrp: 0.392 ± 0.022	0.307TrpTyr: 0.307 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.295TyrAla: 2.295 ± 0.045	0.146TyrCys: 0.146 ± 0.011	1.338TyrAsp: 1.338 ± 0.036	1.005TyrGlu: 1.005 ± 0.033	0.547TyrPhe: 0.547 ± 0.021	1.779TyrGly: 1.779 ± 0.045	0.316TyrHis: 0.316 ± 0.017	0.351TyrIle: 0.351 ± 0.017	0.319TyrLys: 0.319 ± 0.016	2.054TyrLeu: 2.054 ± 0.044	0.191TyrMet: 0.191 ± 0.011	0.35TyrAsn: 0.35 ± 0.02	0.943TyrPro: 0.943 ± 0.032	0.5TyrGln: 0.5 ± 0.021	1.46TyrArg: 1.46 ± 0.037	0.974TyrSer: 0.974 ± 0.03	1.056TyrThr: 1.056 ± 0.028	1.774TyrVal: 1.774 ± 0.037	0.284TyrTrp: 0.284 ± 0.014	0.43TyrTyr: 0.43 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3765 proteins (1280276 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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