Capybara microvirus Cap1_SP_124
Average proteome isoelectric point is 7.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7V9|A0A4P8W7V9_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_124 OX=2585379 PE=4 SV=1
MM1 pKa = 7.35 SRR3 pKa = 11.84 NEE5 pKa = 3.64 LAYY8 pKa = 10.03 EE9 pKa = 4.04 VLDD12 pKa = 4.63 TIDD15 pKa = 3.87 TPRR18 pKa = 11.84 STWQRR23 pKa = 11.84 NFTHH27 pKa = 5.98 KK28 pKa = 9.74 TSFNAGKK35 pKa = 10.27 LIPFYY40 pKa = 10.25 IDD42 pKa = 2.91 TDD44 pKa = 4.38 IIPGTTIKK52 pKa = 10.78 NKK54 pKa = 7.91 TSIVIRR60 pKa = 11.84 MSTPIAPVMDD70 pKa = 4.2 NLYY73 pKa = 10.83 LDD75 pKa = 4.06 TYY77 pKa = 10.16 YY78 pKa = 10.85 FKK80 pKa = 10.92 CSKK83 pKa = 9.39 FWYY86 pKa = 8.05 WEE88 pKa = 3.68 HH89 pKa = 6.45 FRR91 pKa = 11.84 AQMGEE96 pKa = 4.08 NNQSAWTQTIEE107 pKa = 4.47 YY108 pKa = 9.45 IEE110 pKa = 4.36 PQIKK114 pKa = 8.49 MLSMEE119 pKa = 4.1 YY120 pKa = 10.41 GANDD124 pKa = 3.34 VACYY128 pKa = 9.56 MGMPINVANLEE139 pKa = 4.18 WSKK142 pKa = 11.35 LPVQAYY148 pKa = 9.67 IDD150 pKa = 3.21 IWNNWFRR157 pKa = 11.84 DD158 pKa = 3.68 EE159 pKa = 4.63 NLQAPITLDD168 pKa = 3.21 KK169 pKa = 11.01 TDD171 pKa = 4.44 ADD173 pKa = 4.01 LTADD177 pKa = 3.42 ATINTGFGLLPVCKK191 pKa = 9.86 FHH193 pKa = 8.39 DD194 pKa = 4.49 YY195 pKa = 8.25 FTSLLPQPQKK205 pKa = 11.38 GEE207 pKa = 4.09 AVTMPLGLTAPVTGTVIGNGKK228 pKa = 7.37 TLGLWNGTTTAGIRR242 pKa = 11.84 QGIGVSGDD250 pKa = 3.22 NYY252 pKa = 11.04 SNGIGIASYY261 pKa = 10.98 NSSNLPYY268 pKa = 8.63 NTSGVSSLNNTAGLGVTTNPDD289 pKa = 2.93 KK290 pKa = 11.38 SGLKK294 pKa = 9.37 IASNAVADD302 pKa = 4.19 LTSATAATFNALRR315 pKa = 11.84 LAGATQRR322 pKa = 11.84 ILEE325 pKa = 4.15 KK326 pKa = 10.39 DD327 pKa = 3.0 ARR329 pKa = 11.84 FGTRR333 pKa = 11.84 YY334 pKa = 10.0 PEE336 pKa = 4.04 FLKK339 pKa = 10.96 GQYY342 pKa = 10.34 GVTAADD348 pKa = 3.67 EE349 pKa = 4.4 CLLIPEE355 pKa = 4.23 YY356 pKa = 10.85 LGGKK360 pKa = 9.42 RR361 pKa = 11.84 IPINIEE367 pKa = 3.84 QVCQTSSTDD376 pKa = 3.15 STSPLGEE383 pKa = 4.06 TGAMSVTADD392 pKa = 3.13 INEE395 pKa = 4.73 DD396 pKa = 3.43 FTKK399 pKa = 10.84 SFTKK403 pKa = 10.11 HH404 pKa = 6.27 DD405 pKa = 3.95 FLIGVCCVRR414 pKa = 11.84 AEE416 pKa = 3.94 HH417 pKa = 6.96 TYY419 pKa = 9.94 QQGIPLQFKK428 pKa = 9.92 RR429 pKa = 11.84 KK430 pKa = 9.03 RR431 pKa = 11.84 RR432 pKa = 11.84 LDD434 pKa = 3.49 TYY436 pKa = 10.51 HH437 pKa = 7.55 PSLAHH442 pKa = 6.92 IGNQPVYY449 pKa = 10.03 NFEE452 pKa = 4.34 IFAQGNSTDD461 pKa = 4.19 DD462 pKa = 3.11 EE463 pKa = 4.97 VMGYY467 pKa = 9.92 KK468 pKa = 9.81 EE469 pKa = 3.85 AWQEE473 pKa = 3.68 YY474 pKa = 9.17 LYY476 pKa = 10.9 KK477 pKa = 10.26 PNRR480 pKa = 11.84 ISGQMLSTYY489 pKa = 7.29 TQSLDD494 pKa = 2.93 VWHH497 pKa = 6.74 YY498 pKa = 10.96 GDD500 pKa = 5.8 DD501 pKa = 4.11 YY502 pKa = 12.05 VNLPVLSSEE511 pKa = 4.47 WIVEE515 pKa = 3.72 PTEE518 pKa = 4.26 YY519 pKa = 9.51 IDD521 pKa = 3.24 RR522 pKa = 11.84 TLAVSSSVSNQFWADD537 pKa = 2.99 IYY539 pKa = 10.83 IEE541 pKa = 4.41 QIVSAPIPINRR552 pKa = 11.84 VPGLIDD558 pKa = 3.56 HH559 pKa = 6.51 YY560 pKa = 11.72
Molecular weight: 62.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.828
IPC2_protein 4.978
IPC_protein 4.94
Toseland 4.851
ProMoST 5.08
Dawson 4.927
Bjellqvist 5.067
Wikipedia 4.813
Rodwell 4.825
Grimsley 4.774
Solomon 4.927
Lehninger 4.889
Nozaki 5.041
DTASelect 5.232
Thurlkill 4.851
EMBOSS 4.851
Sillero 5.105
Patrickios 3.325
IPC_peptide 4.94
IPC2_peptide 5.105
IPC2.peptide.svr19 5.04
Protein with the highest isoelectric point:
>tr|A0A4V1FVP3|A0A4V1FVP3_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_124 OX=2585379 PE=4 SV=1
MM1 pKa = 7.33 TRR3 pKa = 11.84 LSHH6 pKa = 5.15 NQYY9 pKa = 9.69 YY10 pKa = 10.19 GEE12 pKa = 4.11 RR13 pKa = 11.84 KK14 pKa = 9.76 NVFEE18 pKa = 4.67 MLNIVYY24 pKa = 9.35 RR25 pKa = 11.84 KK26 pKa = 9.01 PSQYY30 pKa = 10.14 HH31 pKa = 5.24 HH32 pKa = 7.3 AGHH35 pKa = 7.07 KK36 pKa = 9.21 EE37 pKa = 3.87 PQRR40 pKa = 11.84 LSHH43 pKa = 6.24 RR44 pKa = 11.84 LKK46 pKa = 8.6 WYY48 pKa = 9.21 YY49 pKa = 11.38 YY50 pKa = 10.06 KK51 pKa = 10.21 FTPEE55 pKa = 4.02 KK56 pKa = 10.18 IAIEE60 pKa = 3.91 NEE62 pKa = 3.47 INRR65 pKa = 11.84 NQRR68 pKa = 11.84 PNIQNEE74 pKa = 4.16 KK75 pKa = 10.4
Molecular weight: 9.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.147
IPC2_protein 9.56
IPC_protein 9.692
Toseland 9.882
ProMoST 9.78
Dawson 10.16
Bjellqvist 9.867
Wikipedia 10.365
Rodwell 10.438
Grimsley 10.262
Solomon 10.189
Lehninger 10.145
Nozaki 9.838
DTASelect 9.867
Thurlkill 9.97
EMBOSS 10.306
Sillero 10.058
Patrickios 7.571
IPC_peptide 10.189
IPC2_peptide 8.536
IPC2.peptide.svr19 8.572
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1502
75
560
250.3
28.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.325 ± 1.08
1.265 ± 0.364
4.594 ± 0.821
7.39 ± 1.182
3.995 ± 0.435
6.591 ± 1.238
1.598 ± 0.448
6.991 ± 0.746
9.521 ± 2.208
7.19 ± 0.621
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.597 ± 0.385
7.39 ± 0.512
3.662 ± 0.743
3.995 ± 0.245
4.194 ± 0.565
5.992 ± 1.357
7.523 ± 0.733
3.129 ± 0.836
1.265 ± 0.376
4.794 ± 0.442
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here