Sewage-associated circular DNA virus-8

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075J186|A0A075J186_9VIRU Uncharacterized protein OS=Sewage-associated circular DNA virus-8 OX=1519397 PE=4 SV=1
MM1 pKa = 7.19SQDD4 pKa = 4.79PLWKK8 pKa = 8.75MQGRR12 pKa = 11.84YY13 pKa = 7.51WMLTAPAASFTPYY26 pKa = 10.61LPPTVLYY33 pKa = 10.35IRR35 pKa = 11.84GQLEE39 pKa = 3.78TAASGFSHH47 pKa = 6.36WQLLVITGKK56 pKa = 9.97VRR58 pKa = 11.84GSKK61 pKa = 10.39LKK63 pKa = 9.97TIFGDD68 pKa = 3.83DD69 pKa = 3.31VHH71 pKa = 8.76IEE73 pKa = 4.12LTRR76 pKa = 11.84SAAADD81 pKa = 3.77DD82 pKa = 4.54YY83 pKa = 11.28VWKK86 pKa = 10.46DD87 pKa = 3.19DD88 pKa = 3.88TYY90 pKa = 11.14VDD92 pKa = 3.52GTRR95 pKa = 11.84FEE97 pKa = 4.4LGRR100 pKa = 11.84RR101 pKa = 11.84PKK103 pKa = 10.19QLSKK107 pKa = 8.32TTDD110 pKa = 2.67WDD112 pKa = 4.93DD113 pKa = 2.86IWKK116 pKa = 10.27SAVDD120 pKa = 4.06GNLEE124 pKa = 4.52DD125 pKa = 3.92IEE127 pKa = 4.66SHH129 pKa = 5.59VRR131 pKa = 11.84VRR133 pKa = 11.84YY134 pKa = 9.25YY135 pKa = 11.22NSLSRR140 pKa = 11.84IRR142 pKa = 11.84QDD144 pKa = 3.03HH145 pKa = 6.5LRR147 pKa = 11.84PIPLEE152 pKa = 4.17RR153 pKa = 11.84SCSVYY158 pKa = 9.73WGRR161 pKa = 11.84TGTGKK166 pKa = 9.99SRR168 pKa = 11.84RR169 pKa = 11.84AWEE172 pKa = 4.11EE173 pKa = 3.42ASMDD177 pKa = 4.16AYY179 pKa = 10.06PKK181 pKa = 10.53NPRR184 pKa = 11.84TKK186 pKa = 9.85FWDD189 pKa = 4.38GYY191 pKa = 9.67RR192 pKa = 11.84GHH194 pKa = 6.02EE195 pKa = 3.93HH196 pKa = 5.99VVIDD200 pKa = 4.17EE201 pKa = 4.11FRR203 pKa = 11.84GGIDD207 pKa = 3.14IAYY210 pKa = 7.76MLQWLDD216 pKa = 3.57RR217 pKa = 11.84YY218 pKa = 9.78PVIVEE223 pKa = 4.03VKK225 pKa = 10.35GSSTVLRR232 pKa = 11.84ATRR235 pKa = 11.84IWITSNIDD243 pKa = 3.03PRR245 pKa = 11.84DD246 pKa = 3.76WYY248 pKa = 10.27PLADD252 pKa = 4.06QEE254 pKa = 4.92TKK256 pKa = 10.77DD257 pKa = 3.45ALLRR261 pKa = 11.84RR262 pKa = 11.84LNITHH267 pKa = 7.31FLL269 pKa = 3.74

Molecular weight: 31.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075J472|A0A075J472_9VIRU Uncharacterized protein OS=Sewage-associated circular DNA virus-8 OX=1519397 PE=4 SV=1
MM1 pKa = 7.51SCNSTLQVLFQPGILRR17 pKa = 11.84GSSPIFSIDD26 pKa = 3.21TLNTFDD32 pKa = 4.85PLLTEE37 pKa = 4.65LVPSFISANRR47 pKa = 11.84AARR50 pKa = 11.84SPLGSRR56 pKa = 11.84AVTFFGAKK64 pKa = 8.38TKK66 pKa = 10.76NFSLYY71 pKa = 10.87ARR73 pKa = 11.84LEE75 pKa = 4.04LFLNVTWQLEE85 pKa = 4.54SITTIFKK92 pKa = 9.79PSLWKK97 pKa = 9.19STPEE101 pKa = 4.05SYY103 pKa = 10.52PGTEE107 pKa = 4.27DD108 pKa = 3.61DD109 pKa = 3.96GSVRR113 pKa = 11.84FLHH116 pKa = 6.6KK117 pKa = 10.36IEE119 pKa = 4.3AASLNEE125 pKa = 3.84FGVNAKK131 pKa = 7.62GTPKK135 pKa = 10.67YY136 pKa = 10.18STFCLLSNTLPPNSGVPPNHH156 pKa = 6.74RR157 pKa = 11.84LEE159 pKa = 4.42TTFFVYY165 pKa = 10.59LPVTSPGANNRR176 pKa = 11.84LIASFMAGFNLTTFFRR192 pKa = 11.84FCLRR196 pKa = 11.84TFPRR200 pKa = 11.84RR201 pKa = 11.84TLPAA205 pKa = 4.75

Molecular weight: 22.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

913

205

439

304.3

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.791 ± 0.4

0.548 ± 0.316

5.257 ± 1.517

4.491 ± 0.213

5.257 ± 1.682

8.653 ± 1.652

1.752 ± 0.377

4.381 ± 0.595

5.696 ± 0.953

8.105 ± 1.509

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.972 ± 0.368

4.272 ± 1.045

5.696 ± 0.903

3.286 ± 0.689

7.338 ± 0.816

7.558 ± 0.933

6.243 ± 1.535

6.462 ± 0.811

2.3 ± 0.861

3.943 ± 0.709
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski