Streptococcus phage SW31

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Brussowvirus; unclassified Brussowvirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A451EGR7|A0A451EGR7_9CAUD XRE family transcriptional regulator OS=Streptococcus phage SW31 OX=2419651 GN=SW31_028 PE=4 SV=1
MM1 pKa = 6.11TTKK4 pKa = 9.29TQLLNTLDD12 pKa = 3.64SLVNQRR18 pKa = 11.84VTVPTNPFGGQCIALIDD35 pKa = 4.58NVLQYY40 pKa = 10.89QGLFNLDD47 pKa = 3.63FSYY50 pKa = 11.48LNAIDD55 pKa = 3.89ALSRR59 pKa = 11.84AEE61 pKa = 4.04SLGLKK66 pKa = 6.96VTRR69 pKa = 11.84FNGANNPTVGSVWVTNCLPYY89 pKa = 10.51HH90 pKa = 5.36QFGHH94 pKa = 6.24IGFVYY99 pKa = 10.67AEE101 pKa = 4.39NPDD104 pKa = 3.63GTVTTIEE111 pKa = 3.95QNIDD115 pKa = 3.02GNADD119 pKa = 3.22SLYY122 pKa = 10.62NGGWTRR128 pKa = 11.84KK129 pKa = 5.87VTRR132 pKa = 11.84NLDD135 pKa = 3.39SAGNFSYY142 pKa = 10.72IDD144 pKa = 3.43WNAPTQQMVGWFEE157 pKa = 4.33LPLDD161 pKa = 4.01GMEE164 pKa = 4.43KK165 pKa = 10.65DD166 pKa = 3.73NYY168 pKa = 10.34FIDD171 pKa = 3.66VSAYY175 pKa = 9.95QPGDD179 pKa = 3.26LTAICQASGTNNTVIKK195 pKa = 9.37VTEE198 pKa = 4.14GVGWVSPVAAQQTNTSNCIGYY219 pKa = 7.92YY220 pKa = 9.85HH221 pKa = 7.1FARR224 pKa = 11.84FGGDD228 pKa = 2.81VATAQAEE235 pKa = 4.18ANYY238 pKa = 9.8FISNLPSHH246 pKa = 6.82PRR248 pKa = 11.84YY249 pKa = 9.38LVCDD253 pKa = 4.05YY254 pKa = 11.33EE255 pKa = 6.63DD256 pKa = 4.21GASSDD261 pKa = 3.44KK262 pKa = 10.65QANTNAVLAFMDD274 pKa = 3.3ICKK277 pKa = 10.71ANGFEE282 pKa = 4.51PIYY285 pKa = 10.66YY286 pKa = 9.81SYY288 pKa = 11.36KK289 pKa = 10.34PYY291 pKa = 10.33TLANVYY297 pKa = 9.91VDD299 pKa = 4.33QITARR304 pKa = 11.84YY305 pKa = 7.8PNSLWIAAYY314 pKa = 8.94PDD316 pKa = 3.74YY317 pKa = 10.64EE318 pKa = 4.52VRR320 pKa = 11.84PEE322 pKa = 4.38PYY324 pKa = 9.07WGVYY328 pKa = 10.28PNMEE332 pKa = 4.23HH333 pKa = 5.47TRR335 pKa = 11.84WWQFTSTGLAGGLDD349 pKa = 3.79KK350 pKa = 11.4NIVIINDD357 pKa = 3.44DD358 pKa = 4.28DD359 pKa = 4.33NLVNQKK365 pKa = 9.68EE366 pKa = 4.45EE367 pKa = 3.98EE368 pKa = 4.64DD369 pKa = 3.22IMNFVVRR376 pKa = 11.84SEE378 pKa = 4.14SGKK381 pKa = 9.02EE382 pKa = 3.54GWVAVVNGRR391 pKa = 11.84VFGIGSMGTVDD402 pKa = 4.02ALEE405 pKa = 4.13ATGAKK410 pKa = 9.84RR411 pKa = 11.84LQLEE415 pKa = 4.23DD416 pKa = 3.99ADD418 pKa = 4.51FNRR421 pKa = 11.84FLYY424 pKa = 10.37SQSNDD429 pKa = 2.85TAAVAKK435 pKa = 10.67AIDD438 pKa = 3.8EE439 pKa = 4.55ASASVVKK446 pKa = 10.68AIEE449 pKa = 3.89EE450 pKa = 4.05RR451 pKa = 11.84AQATQGQTGKK461 pKa = 10.76

Molecular weight: 50.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S5X4M6|A0A3S5X4M6_9CAUD Uncharacterized protein OS=Streptococcus phage SW31 OX=2419651 GN=SW31_023 PE=4 SV=1
MM1 pKa = 7.83ADD3 pKa = 3.75FGVSLLEE10 pKa = 4.01ARR12 pKa = 11.84RR13 pKa = 11.84MTLKK17 pKa = 10.49EE18 pKa = 3.53MKK20 pKa = 9.92LYY22 pKa = 10.32QKK24 pKa = 10.55AYY26 pKa = 9.84KK27 pKa = 10.03KK28 pKa = 10.52RR29 pKa = 11.84FLNKK33 pKa = 9.43EE34 pKa = 3.69RR35 pKa = 11.84EE36 pKa = 4.36IYY38 pKa = 9.68QLAYY42 pKa = 10.87LNRR45 pKa = 11.84LANATTKK52 pKa = 10.62DD53 pKa = 3.03GKK55 pKa = 10.64KK56 pKa = 10.46YY57 pKa = 10.49YY58 pKa = 10.19FEE60 pKa = 5.48KK61 pKa = 10.83FDD63 pKa = 3.95DD64 pKa = 4.49FYY66 pKa = 11.56NAKK69 pKa = 9.54EE70 pKa = 3.83RR71 pKa = 11.84ARR73 pKa = 11.84EE74 pKa = 3.84VLGEE78 pKa = 4.35KK79 pKa = 8.61ITNSKK84 pKa = 10.18LLEE87 pKa = 3.98RR88 pKa = 11.84ARR90 pKa = 11.84NNLNYY95 pKa = 10.06KK96 pKa = 9.85KK97 pKa = 10.46EE98 pKa = 3.94RR99 pKa = 11.84GLLDD103 pKa = 3.22GRR105 pKa = 4.46

Molecular weight: 12.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

11139

48

1609

271.7

30.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.685 ± 0.955

0.44 ± 0.112

5.97 ± 0.438

6.715 ± 0.678

3.932 ± 0.176

6.859 ± 0.528

1.23 ± 0.164

6.724 ± 0.362

7.721 ± 0.552

7.909 ± 0.377

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.343 ± 0.358

5.853 ± 0.373

3.052 ± 0.242

3.959 ± 0.24

4.273 ± 0.393

6.913 ± 0.653

6.616 ± 0.332

6.625 ± 0.201

1.329 ± 0.209

3.851 ± 0.448
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski