Amino acid dipepetide frequency for Streptococcus phage SW31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.836AlaAla: 5.836 ± 1.63
0.18AlaCys: 0.18 ± 0.145
4.758AlaAsp: 4.758 ± 0.649
3.771AlaGlu: 3.771 ± 0.654
2.783AlaPhe: 2.783 ± 1.33
5.207AlaGly: 5.207 ± 1.284
0.808AlaHis: 0.808 ± 0.294
6.823AlaIle: 6.823 ± 1.48
5.387AlaLys: 5.387 ± 0.794
6.644AlaLeu: 6.644 ± 0.784
2.334AlaMet: 2.334 ± 1.145
4.399AlaAsn: 4.399 ± 0.878
2.424AlaPro: 2.424 ± 0.401
2.963AlaGln: 2.963 ± 0.827
3.591AlaArg: 3.591 ± 0.678
6.734AlaSer: 6.734 ± 1.47
4.13AlaThr: 4.13 ± 0.879
4.399AlaVal: 4.399 ± 1.15
0.808AlaTrp: 0.808 ± 0.385
2.873AlaTyr: 2.873 ± 0.596
0.0AlaXaa: 0.0 ± 0.0
Cys
0.269CysAla: 0.269 ± 0.133
0.0CysCys: 0.0 ± 0.0
0.359CysAsp: 0.359 ± 0.186
0.18CysGlu: 0.18 ± 0.124
0.09CysPhe: 0.09 ± 0.101
0.269CysGly: 0.269 ± 0.208
0.09CysHis: 0.09 ± 0.081
0.449CysIle: 0.449 ± 0.192
0.449CysLys: 0.449 ± 0.232
0.359CysLeu: 0.359 ± 0.23
0.09CysMet: 0.09 ± 0.097
0.269CysAsn: 0.269 ± 0.173
0.09CysPro: 0.09 ± 0.079
0.18CysGln: 0.18 ± 0.12
0.18CysArg: 0.18 ± 0.156
0.269CysSer: 0.269 ± 0.157
0.0CysThr: 0.0 ± 0.0
0.359CysVal: 0.359 ± 0.172
0.0CysTrp: 0.0 ± 0.0
0.449CysTyr: 0.449 ± 0.341
0.0CysXaa: 0.0 ± 0.0
Asp
3.232AspAla: 3.232 ± 0.542
0.18AspCys: 0.18 ± 0.118
4.399AspAsp: 4.399 ± 0.71
3.502AspGlu: 3.502 ± 0.511
3.322AspPhe: 3.322 ± 0.622
6.105AspGly: 6.105 ± 1.131
0.449AspHis: 0.449 ± 0.239
3.053AspIle: 3.053 ± 0.441
4.489AspLys: 4.489 ± 0.805
4.31AspLeu: 4.31 ± 0.776
1.796AspMet: 1.796 ± 0.36
4.22AspAsn: 4.22 ± 0.871
0.718AspPro: 0.718 ± 0.254
1.526AspGln: 1.526 ± 0.352
2.514AspArg: 2.514 ± 0.411
4.399AspSer: 4.399 ± 0.501
3.502AspThr: 3.502 ± 0.682
3.322AspVal: 3.322 ± 0.597
1.077AspTrp: 1.077 ± 0.324
3.591AspTyr: 3.591 ± 0.825
0.0AspXaa: 0.0 ± 0.0
Glu
4.579GluAla: 4.579 ± 0.86
0.269GluCys: 0.269 ± 0.14
2.245GluAsp: 2.245 ± 0.42
4.13GluGlu: 4.13 ± 0.847
2.873GluPhe: 2.873 ± 0.535
3.502GluGly: 3.502 ± 0.518
1.167GluHis: 1.167 ± 0.395
4.489GluIle: 4.489 ± 0.762
4.848GluLys: 4.848 ± 0.99
7.632GluLeu: 7.632 ± 1.225
2.245GluMet: 2.245 ± 0.568
4.04GluAsn: 4.04 ± 0.627
2.245GluPro: 2.245 ± 0.667
2.514GluGln: 2.514 ± 0.53
3.95GluArg: 3.95 ± 0.802
2.963GluSer: 2.963 ± 0.672
3.861GluThr: 3.861 ± 0.84
4.848GluVal: 4.848 ± 0.748
1.347GluTrp: 1.347 ± 0.363
3.412GluTyr: 3.412 ± 0.726
0.0GluXaa: 0.0 ± 0.0
Phe
2.424PheAla: 2.424 ± 0.453
0.269PheCys: 0.269 ± 0.166
2.783PheAsp: 2.783 ± 0.514
3.322PheGlu: 3.322 ± 0.54
1.167PhePhe: 1.167 ± 0.301
3.232PheGly: 3.232 ± 0.843
0.269PheHis: 0.269 ± 0.162
2.245PheIle: 2.245 ± 0.468
4.579PheLys: 4.579 ± 0.568
1.975PheLeu: 1.975 ± 0.484
1.077PheMet: 1.077 ± 0.272
3.771PheAsn: 3.771 ± 0.505
0.539PhePro: 0.539 ± 0.239
1.077PheGln: 1.077 ± 0.417
1.257PheArg: 1.257 ± 0.306
3.142PheSer: 3.142 ± 0.485
2.604PheThr: 2.604 ± 0.488
1.885PheVal: 1.885 ± 0.405
0.718PheTrp: 0.718 ± 0.313
0.988PheTyr: 0.988 ± 0.312
0.0PheXaa: 0.0 ± 0.0
Gly
5.028GlyAla: 5.028 ± 0.832
0.269GlyCys: 0.269 ± 0.158
3.412GlyAsp: 3.412 ± 0.537
3.232GlyGlu: 3.232 ± 0.468
2.693GlyPhe: 2.693 ± 0.435
3.771GlyGly: 3.771 ± 0.604
0.808GlyHis: 0.808 ± 0.259
6.464GlyIle: 6.464 ± 1.846
5.926GlyLys: 5.926 ± 0.826
6.285GlyLeu: 6.285 ± 0.985
2.065GlyMet: 2.065 ± 0.649
3.502GlyAsn: 3.502 ± 0.664
1.347GlyPro: 1.347 ± 0.673
2.783GlyGln: 2.783 ± 0.443
3.142GlyArg: 3.142 ± 0.675
4.31GlySer: 4.31 ± 0.772
5.656GlyThr: 5.656 ± 0.861
3.861GlyVal: 3.861 ± 0.626
1.437GlyTrp: 1.437 ± 0.464
2.604GlyTyr: 2.604 ± 0.503
0.0GlyXaa: 0.0 ± 0.0
His
0.449HisAla: 0.449 ± 0.236
0.0HisCys: 0.0 ± 0.0
0.898HisAsp: 0.898 ± 0.241
0.808HisGlu: 0.808 ± 0.347
0.628HisPhe: 0.628 ± 0.261
0.808HisGly: 0.808 ± 0.34
0.359HisHis: 0.359 ± 0.179
1.077HisIle: 1.077 ± 0.37
0.988HisLys: 0.988 ± 0.351
1.077HisLeu: 1.077 ± 0.274
0.269HisMet: 0.269 ± 0.173
0.359HisAsn: 0.359 ± 0.217
0.539HisPro: 0.539 ± 0.284
0.359HisGln: 0.359 ± 0.21
0.449HisArg: 0.449 ± 0.177
0.718HisSer: 0.718 ± 0.25
0.808HisThr: 0.808 ± 0.226
0.898HisVal: 0.898 ± 0.316
0.18HisTrp: 0.18 ± 0.141
0.628HisTyr: 0.628 ± 0.21
0.0HisXaa: 0.0 ± 0.0
Ile
5.746IleAla: 5.746 ± 1.191
0.449IleCys: 0.449 ± 0.25
5.477IleAsp: 5.477 ± 0.675
4.22IleGlu: 4.22 ± 0.744
1.257IlePhe: 1.257 ± 0.44
5.387IleGly: 5.387 ± 0.946
0.808IleHis: 0.808 ± 0.236
3.771IleIle: 3.771 ± 0.752
5.118IleLys: 5.118 ± 0.656
4.04IleLeu: 4.04 ± 0.569
1.706IleMet: 1.706 ± 0.413
3.142IleAsn: 3.142 ± 0.516
2.334IlePro: 2.334 ± 0.431
3.591IleGln: 3.591 ± 0.553
2.873IleArg: 2.873 ± 0.482
5.656IleSer: 5.656 ± 1.135
3.95IleThr: 3.95 ± 0.633
3.591IleVal: 3.591 ± 0.748
0.539IleTrp: 0.539 ± 0.215
3.591IleTyr: 3.591 ± 0.777
0.0IleXaa: 0.0 ± 0.0
Lys
7.452LysAla: 7.452 ± 0.819
0.18LysCys: 0.18 ± 0.124
3.861LysAsp: 3.861 ± 0.935
7.183LysGlu: 7.183 ± 1.241
2.424LysPhe: 2.424 ± 0.496
5.297LysGly: 5.297 ± 0.672
0.808LysHis: 0.808 ± 0.336
4.22LysIle: 4.22 ± 0.937
5.567LysLys: 5.567 ± 1.149
6.105LysLeu: 6.105 ± 0.88
1.347LysMet: 1.347 ± 0.403
4.22LysAsn: 4.22 ± 0.736
3.232LysPro: 3.232 ± 0.521
1.975LysGln: 1.975 ± 0.513
4.669LysArg: 4.669 ± 0.87
4.579LysSer: 4.579 ± 0.594
6.015LysThr: 6.015 ± 0.853
4.31LysVal: 4.31 ± 0.604
0.898LysTrp: 0.898 ± 0.315
2.873LysTyr: 2.873 ± 0.64
0.0LysXaa: 0.0 ± 0.0
Leu
6.375LeuAla: 6.375 ± 0.769
0.449LeuCys: 0.449 ± 0.231
4.938LeuAsp: 4.938 ± 0.778
7.003LeuGlu: 7.003 ± 1.296
2.334LeuPhe: 2.334 ± 0.386
5.118LeuGly: 5.118 ± 0.872
0.539LeuHis: 0.539 ± 0.218
4.399LeuIle: 4.399 ± 0.782
6.464LeuLys: 6.464 ± 0.94
4.579LeuLeu: 4.579 ± 0.738
1.706LeuMet: 1.706 ± 0.377
5.746LeuAsn: 5.746 ± 0.541
2.873LeuPro: 2.873 ± 0.629
3.142LeuGln: 3.142 ± 0.433
3.232LeuArg: 3.232 ± 0.699
5.567LeuSer: 5.567 ± 0.74
6.195LeuThr: 6.195 ± 0.789
5.028LeuVal: 5.028 ± 0.648
0.628LeuTrp: 0.628 ± 0.277
2.693LeuTyr: 2.693 ± 0.534
0.0LeuXaa: 0.0 ± 0.0
Met
2.514MetAla: 2.514 ± 0.775
0.09MetCys: 0.09 ± 0.078
0.718MetAsp: 0.718 ± 0.269
1.616MetGlu: 1.616 ± 0.394
1.167MetPhe: 1.167 ± 0.367
1.257MetGly: 1.257 ± 0.616
0.449MetHis: 0.449 ± 0.178
1.257MetIle: 1.257 ± 0.439
2.155MetLys: 2.155 ± 0.4
1.526MetLeu: 1.526 ± 0.399
0.808MetMet: 0.808 ± 0.624
0.988MetAsn: 0.988 ± 0.249
0.628MetPro: 0.628 ± 0.23
1.616MetGln: 1.616 ± 0.623
0.808MetArg: 0.808 ± 0.242
1.616MetSer: 1.616 ± 0.524
1.706MetThr: 1.706 ± 0.394
1.885MetVal: 1.885 ± 0.405
0.09MetTrp: 0.09 ± 0.098
0.539MetTyr: 0.539 ± 0.312
0.0MetXaa: 0.0 ± 0.0
Asn
4.04AsnAla: 4.04 ± 0.85
0.269AsnCys: 0.269 ± 0.201
3.053AsnAsp: 3.053 ± 0.568
4.04AsnGlu: 4.04 ± 1.043
2.334AsnPhe: 2.334 ± 0.566
6.375AsnGly: 6.375 ± 0.987
1.257AsnHis: 1.257 ± 0.371
3.591AsnIle: 3.591 ± 0.564
3.861AsnLys: 3.861 ± 0.578
4.489AsnLeu: 4.489 ± 0.549
1.167AsnMet: 1.167 ± 0.275
3.502AsnAsn: 3.502 ± 0.64
2.693AsnPro: 2.693 ± 0.391
1.975AsnGln: 1.975 ± 0.479
2.065AsnArg: 2.065 ± 0.584
2.693AsnSer: 2.693 ± 0.39
3.591AsnThr: 3.591 ± 0.656
3.95AsnVal: 3.95 ± 0.509
1.616AsnTrp: 1.616 ± 0.483
1.975AsnTyr: 1.975 ± 0.464
0.0AsnXaa: 0.0 ± 0.0
Pro
1.975ProAla: 1.975 ± 0.568
0.18ProCys: 0.18 ± 0.121
2.155ProAsp: 2.155 ± 0.528
1.796ProGlu: 1.796 ± 0.511
1.167ProPhe: 1.167 ± 0.312
1.167ProGly: 1.167 ± 0.372
0.449ProHis: 0.449 ± 0.27
1.885ProIle: 1.885 ± 0.345
3.142ProLys: 3.142 ± 0.518
1.975ProLeu: 1.975 ± 0.543
0.18ProMet: 0.18 ± 0.118
2.155ProAsn: 2.155 ± 0.484
1.167ProPro: 1.167 ± 0.34
1.167ProGln: 1.167 ± 0.505
1.167ProArg: 1.167 ± 0.319
3.053ProSer: 3.053 ± 0.484
1.526ProThr: 1.526 ± 0.453
2.514ProVal: 2.514 ± 0.417
0.269ProTrp: 0.269 ± 0.116
1.437ProTyr: 1.437 ± 0.438
0.0ProXaa: 0.0 ± 0.0
Gln
3.861GlnAla: 3.861 ± 0.84
0.359GlnCys: 0.359 ± 0.144
1.796GlnAsp: 1.796 ± 0.484
2.963GlnGlu: 2.963 ± 0.683
1.616GlnPhe: 1.616 ± 0.382
2.873GlnGly: 2.873 ± 0.96
0.449GlnHis: 0.449 ± 0.204
2.424GlnIle: 2.424 ± 0.559
2.155GlnLys: 2.155 ± 0.404
3.771GlnLeu: 3.771 ± 0.691
0.539GlnMet: 0.539 ± 0.265
1.975GlnAsn: 1.975 ± 0.341
1.167GlnPro: 1.167 ± 0.387
1.167GlnGln: 1.167 ± 0.308
1.347GlnArg: 1.347 ± 0.386
3.412GlnSer: 3.412 ± 0.903
3.232GlnThr: 3.232 ± 0.493
2.693GlnVal: 2.693 ± 0.553
0.539GlnTrp: 0.539 ± 0.256
1.257GlnTyr: 1.257 ± 0.351
0.0GlnXaa: 0.0 ± 0.0
Arg
3.412ArgAla: 3.412 ± 0.505
0.449ArgCys: 0.449 ± 0.252
2.693ArgAsp: 2.693 ± 0.462
2.693ArgGlu: 2.693 ± 0.668
2.065ArgPhe: 2.065 ± 0.546
2.783ArgGly: 2.783 ± 0.41
0.449ArgHis: 0.449 ± 0.205
3.232ArgIle: 3.232 ± 0.724
3.232ArgLys: 3.232 ± 0.862
3.142ArgLeu: 3.142 ± 0.643
1.706ArgMet: 1.706 ± 0.379
2.155ArgAsn: 2.155 ± 0.478
1.077ArgPro: 1.077 ± 0.296
1.706ArgGln: 1.706 ± 0.404
1.975ArgArg: 1.975 ± 0.491
2.155ArgSer: 2.155 ± 0.42
2.065ArgThr: 2.065 ± 0.428
2.693ArgVal: 2.693 ± 0.596
0.898ArgTrp: 0.898 ± 0.284
2.155ArgTyr: 2.155 ± 0.437
0.0ArgXaa: 0.0 ± 0.0
Ser
6.644SerAla: 6.644 ± 2.583
0.18SerCys: 0.18 ± 0.107
3.95SerAsp: 3.95 ± 0.647
3.861SerGlu: 3.861 ± 0.714
2.783SerPhe: 2.783 ± 0.587
4.31SerGly: 4.31 ± 0.777
0.628SerHis: 0.628 ± 0.246
5.297SerIle: 5.297 ± 0.782
4.669SerLys: 4.669 ± 0.628
6.195SerLeu: 6.195 ± 0.903
1.437SerMet: 1.437 ± 0.263
4.31SerAsn: 4.31 ± 0.659
1.885SerPro: 1.885 ± 0.368
3.681SerGln: 3.681 ± 0.772
1.885SerArg: 1.885 ± 0.473
4.669SerSer: 4.669 ± 1.271
4.399SerThr: 4.399 ± 0.662
5.746SerVal: 5.746 ± 1.028
0.449SerTrp: 0.449 ± 0.201
2.155SerTyr: 2.155 ± 0.456
0.0SerXaa: 0.0 ± 0.0
Thr
4.579ThrAla: 4.579 ± 1.386
0.09ThrCys: 0.09 ± 0.079
3.412ThrAsp: 3.412 ± 0.697
3.95ThrGlu: 3.95 ± 0.732
3.861ThrPhe: 3.861 ± 0.568
4.04ThrGly: 4.04 ± 0.683
1.167ThrHis: 1.167 ± 0.344
4.758ThrIle: 4.758 ± 0.822
5.567ThrLys: 5.567 ± 0.911
6.015ThrLeu: 6.015 ± 0.82
0.988ThrMet: 0.988 ± 0.579
2.963ThrAsn: 2.963 ± 0.561
1.796ThrPro: 1.796 ± 0.453
3.142ThrGln: 3.142 ± 0.526
2.424ThrArg: 2.424 ± 0.542
4.399ThrSer: 4.399 ± 1.236
4.399ThrThr: 4.399 ± 0.637
6.285ThrVal: 6.285 ± 0.775
0.269ThrTrp: 0.269 ± 0.164
2.065ThrTyr: 2.065 ± 0.546
0.0ThrXaa: 0.0 ± 0.0
Val
4.399ValAla: 4.399 ± 0.853
0.18ValCys: 0.18 ± 0.12
5.387ValAsp: 5.387 ± 0.862
5.477ValGlu: 5.477 ± 1.022
2.514ValPhe: 2.514 ± 0.567
4.13ValGly: 4.13 ± 0.807
0.718ValHis: 0.718 ± 0.227
4.22ValIle: 4.22 ± 0.601
5.387ValLys: 5.387 ± 0.543
4.848ValLeu: 4.848 ± 0.747
0.988ValMet: 0.988 ± 0.251
4.22ValAsn: 4.22 ± 0.591
1.885ValPro: 1.885 ± 0.391
2.245ValGln: 2.245 ± 0.706
2.155ValArg: 2.155 ± 0.453
4.938ValSer: 4.938 ± 0.636
5.028ValThr: 5.028 ± 0.708
5.028ValVal: 5.028 ± 0.719
0.898ValTrp: 0.898 ± 0.221
1.616ValTyr: 1.616 ± 0.43
0.0ValXaa: 0.0 ± 0.0
Trp
0.628TrpAla: 0.628 ± 0.178
0.09TrpCys: 0.09 ± 0.081
0.718TrpAsp: 0.718 ± 0.301
1.077TrpGlu: 1.077 ± 0.285
0.628TrpPhe: 0.628 ± 0.244
0.988TrpGly: 0.988 ± 0.24
0.18TrpHis: 0.18 ± 0.109
1.077TrpIle: 1.077 ± 0.288
0.628TrpLys: 0.628 ± 0.214
0.628TrpLeu: 0.628 ± 0.2
0.18TrpMet: 0.18 ± 0.125
0.808TrpAsn: 0.808 ± 0.484
0.18TrpPro: 0.18 ± 0.126
0.628TrpGln: 0.628 ± 0.278
0.628TrpArg: 0.628 ± 0.27
1.706TrpSer: 1.706 ± 0.594
1.077TrpThr: 1.077 ± 0.268
0.808TrpVal: 0.808 ± 0.313
0.269TrpTrp: 0.269 ± 0.158
0.359TrpTyr: 0.359 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.412TyrAla: 3.412 ± 0.601
0.269TyrCys: 0.269 ± 0.133
2.693TyrAsp: 2.693 ± 0.772
2.334TyrGlu: 2.334 ± 0.544
1.616TyrPhe: 1.616 ± 0.493
1.975TyrGly: 1.975 ± 0.471
0.449TyrHis: 0.449 ± 0.249
2.514TyrIle: 2.514 ± 0.527
2.604TyrLys: 2.604 ± 0.487
3.502TyrLeu: 3.502 ± 0.784
0.718TyrMet: 0.718 ± 0.3
1.796TyrAsn: 1.796 ± 0.417
1.706TyrPro: 1.706 ± 0.521
2.155TyrGln: 2.155 ± 0.378
2.424TyrArg: 2.424 ± 0.715
2.155TyrSer: 2.155 ± 0.451
2.424TyrThr: 2.424 ± 0.65
2.155TyrVal: 2.155 ± 0.4
0.359TyrTrp: 0.359 ± 0.183
1.257TyrTyr: 1.257 ± 0.404
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 41 proteins (11139 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski