Streptococcus sp. 28462
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1977 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3L9DNV8|A0A3L9DNV8_9STRE Threonine synthase OS=Streptococcus sp. 28462 OX=2282420 GN=EAF07_07200 PE=3 SV=1
MM1 pKa = 7.41 LKK3 pKa = 10.26 IIVHH7 pKa = 6.51 AFAEE11 pKa = 4.26 PTEE14 pKa = 4.15 AVVEE18 pKa = 3.97 VLYY21 pKa = 10.89 ASEE24 pKa = 4.51 DD25 pKa = 3.65 EE26 pKa = 4.39 NVISNKK32 pKa = 8.39 MAEE35 pKa = 4.43 FTTQYY40 pKa = 10.44 PNDD43 pKa = 3.64 YY44 pKa = 10.08 LAVYY48 pKa = 9.43 DD49 pKa = 5.02 LPLDD53 pKa = 3.67 VDD55 pKa = 4.77 LGSLPHH61 pKa = 6.22 YY62 pKa = 9.37 PSVAISPDD70 pKa = 3.52 DD71 pKa = 3.84 FNGGEE76 pKa = 4.25 GG77 pKa = 3.41
Molecular weight: 8.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.807
IPC2_protein 3.821
IPC_protein 3.719
Toseland 3.541
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.465
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.012
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.846
Patrickios 0.401
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|A0A3L9DKZ5|A0A3L9DKZ5_9STRE Uncharacterized protein OS=Streptococcus sp. 28462 OX=2282420 GN=EAF07_08060 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.67 IRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 KK15 pKa = 7.52 HH16 pKa = 5.2 GFRR19 pKa = 11.84 HH20 pKa = 6.39 RR21 pKa = 11.84 MSTKK25 pKa = 9.02 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1977
0
1977
596538
18
2196
301.7
33.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.671 ± 0.072
0.538 ± 0.016
5.784 ± 0.042
7.207 ± 0.064
4.497 ± 0.045
6.621 ± 0.052
1.902 ± 0.025
7.081 ± 0.049
6.725 ± 0.054
9.964 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.498 ± 0.025
4.421 ± 0.038
3.353 ± 0.033
3.873 ± 0.036
3.989 ± 0.043
5.982 ± 0.041
6.075 ± 0.049
7.091 ± 0.05
0.943 ± 0.023
3.785 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here