Aquicella siphonis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae; Aquicella

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2580 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5E4PHL6|A0A5E4PHL6_9COXI Succinate dehydrogenase iron-sulfur subunit OS=Aquicella siphonis OX=254247 GN=sdhB PE=3 SV=1
MM1 pKa = 7.59PLEE4 pKa = 4.33IKK6 pKa = 10.58NIEE9 pKa = 4.12AADD12 pKa = 4.04GSNFAYY18 pKa = 10.4NDD20 pKa = 3.66RR21 pKa = 11.84VVMGAQFNLVNNSSPGAIASVAVDD45 pKa = 3.12AAGSYY50 pKa = 10.57EE51 pKa = 4.24SLPVIGTTGNGSGATLVAKK70 pKa = 9.85MKK72 pKa = 10.8ALTAVVAAAGTGYY85 pKa = 10.9LPGDD89 pKa = 4.53TITLTGGTFTTAAVMTIDD107 pKa = 3.38TSKK110 pKa = 10.86LVSAAVNAAGTGYY123 pKa = 11.04APTDD127 pKa = 3.78TITLAGGVATTAAVLSVATTKK148 pKa = 10.83LISAAVDD155 pKa = 3.65APGSGYY161 pKa = 10.97DD162 pKa = 3.45VADD165 pKa = 4.84TITLAGGAGATKK177 pKa = 10.49AVLTVDD183 pKa = 3.28NAQLVSVALDD193 pKa = 3.58AAGSGYY199 pKa = 11.14APGDD203 pKa = 3.92TITLAGGTAGTKK215 pKa = 10.36AVVTVDD221 pKa = 3.76TVSSGAIATFHH232 pKa = 6.79ISDD235 pKa = 3.75AGDD238 pKa = 3.51YY239 pKa = 9.04TVTATSFTQDD249 pKa = 3.11STSGAGTGATFDD261 pKa = 3.33TGLFGVLDD269 pKa = 4.08FTVSTAGSYY278 pKa = 6.57TTNAAAFTQDD288 pKa = 2.95STTGSGTGATFDD300 pKa = 3.45TTVFGVNTFTIANAGAYY317 pKa = 6.75QTNAASFTQAATSGAGTGATFNTTAYY343 pKa = 10.04GVNTFTISTAGSYY356 pKa = 10.63SVLPSDD362 pKa = 4.75PVAQGSTSGAGTGATFNINTWGLTAVTVSAGGTGYY397 pKa = 11.02DD398 pKa = 3.5SGSDD402 pKa = 3.37VTFTGGGGSGGAAATITLGSQAGNAVTVPVAFAANANLPAEE443 pKa = 4.33YY444 pKa = 10.36GVFVNPGQDD453 pKa = 3.39CRR455 pKa = 11.84WYY457 pKa = 10.95VDD459 pKa = 4.01NNSKK463 pKa = 8.23TATGFNVILTPVTGSDD479 pKa = 3.77VIAAGHH485 pKa = 6.53IDD487 pKa = 3.6VLLVGG492 pKa = 4.54

Molecular weight: 47.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5E4PDT1|A0A5E4PDT1_9COXI Uncharacterized protein OS=Aquicella siphonis OX=254247 GN=AQUSIP_00170 PE=4 SV=1
MM1 pKa = 7.63AKK3 pKa = 10.43AKK5 pKa = 9.14TAAKK9 pKa = 10.03KK10 pKa = 10.21KK11 pKa = 8.59VAKK14 pKa = 9.29PRR16 pKa = 11.84KK17 pKa = 8.22KK18 pKa = 9.99AARR21 pKa = 11.84KK22 pKa = 7.25TKK24 pKa = 9.73VVKK27 pKa = 10.44AKK29 pKa = 10.43KK30 pKa = 9.59AVKK33 pKa = 8.87KK34 pKa = 6.63TTAKK38 pKa = 10.05KK39 pKa = 10.03RR40 pKa = 11.84SAGRR44 pKa = 11.84KK45 pKa = 7.34KK46 pKa = 10.46ARR48 pKa = 11.84PAAKK52 pKa = 9.68KK53 pKa = 10.23KK54 pKa = 10.21KK55 pKa = 9.27AAKK58 pKa = 9.74KK59 pKa = 10.07

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2580

0

2580

845115

30

4636

327.6

36.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.665 ± 0.05

1.142 ± 0.018

5.106 ± 0.033

5.614 ± 0.045

4.203 ± 0.033

6.49 ± 0.045

2.597 ± 0.024

6.96 ± 0.035

5.401 ± 0.046

10.43 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.686 ± 0.024

4.189 ± 0.033

4.402 ± 0.032

4.256 ± 0.035

5.294 ± 0.037

6.594 ± 0.043

5.199 ± 0.036

6.259 ± 0.04

1.129 ± 0.019

3.386 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski