Megasphaera micronuciformis F0359
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1771 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E2ZBB6|E2ZBB6_9FIRM DNA-directed RNA polymerase subunit beta' OS=Megasphaera micronuciformis F0359 OX=706434 GN=rpoC PE=3 SV=1
MM1 pKa = 7.54 ANVSLGMTGTRR12 pKa = 11.84 VASVQNMLIGTGYY25 pKa = 8.81 LTDD28 pKa = 3.93 GADD31 pKa = 3.48 GVFGSGTQAAVQRR44 pKa = 11.84 FQADD48 pKa = 2.92 HH49 pKa = 6.88 GLTADD54 pKa = 5.11 GIVGTQTMNALSTASGQPIPVDD76 pKa = 3.12 NTIVMEE82 pKa = 4.36 ATAYY86 pKa = 8.9 TADD89 pKa = 3.79 DD90 pKa = 4.88 PGNSGYY96 pKa = 8.42 TATGQPLAYY105 pKa = 10.2 GMVSVDD111 pKa = 3.65 PNVIPLGSQLYY122 pKa = 9.95 IEE124 pKa = 5.25 GYY126 pKa = 9.69 GYY128 pKa = 11.17 AVAADD133 pKa = 3.65 TGGGIVGNRR142 pKa = 11.84 IDD144 pKa = 4.14 LAMDD148 pKa = 3.87 SVSDD152 pKa = 3.57 ALNFGRR158 pKa = 11.84 RR159 pKa = 11.84 DD160 pKa = 3.65 VVVHH164 pKa = 5.74 VLL166 pKa = 3.34
Molecular weight: 16.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.91
IPC_protein 3.884
Toseland 3.656
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.91
Rodwell 3.706
Grimsley 3.567
Solomon 3.897
Lehninger 3.846
Nozaki 4.037
DTASelect 4.342
Thurlkill 3.732
EMBOSS 3.91
Sillero 4.012
Patrickios 0.896
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.901
Protein with the highest isoelectric point:
>tr|E2Z9N1|E2Z9N1_9FIRM Serine acetyltransferase OS=Megasphaera micronuciformis F0359 OX=706434 GN=cysE PE=3 SV=1
MM1 pKa = 7.29 KK2 pKa = 9.51 QTFQPNNLWKK12 pKa = 10.57 KK13 pKa = 7.58 RR14 pKa = 11.84 THH16 pKa = 6.26 GFRR19 pKa = 11.84 EE20 pKa = 3.79 RR21 pKa = 11.84 MKK23 pKa = 9.87 TKK25 pKa = 10.01 GGRR28 pKa = 11.84 MVLKK32 pKa = 9.89 KK33 pKa = 10.47 RR34 pKa = 11.84 RR35 pKa = 11.84 MKK37 pKa = 10.56 GRR39 pKa = 11.84 KK40 pKa = 8.82 KK41 pKa = 10.62 LSAA44 pKa = 3.95
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 10.73
IPC_protein 12.237
Toseland 12.427
ProMoST 12.896
Dawson 12.427
Bjellqvist 12.413
Wikipedia 12.896
Rodwell 12.369
Grimsley 12.471
Solomon 12.91
Lehninger 12.808
Nozaki 12.427
DTASelect 12.413
Thurlkill 12.427
EMBOSS 12.925
Sillero 12.427
Patrickios 12.106
IPC_peptide 12.91
IPC2_peptide 11.886
IPC2.peptide.svr19 9.0
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1771
0
1771
534718
37
2811
301.9
33.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.715 ± 0.07
1.245 ± 0.023
5.744 ± 0.048
6.72 ± 0.068
3.913 ± 0.051
7.663 ± 0.064
2.063 ± 0.028
6.665 ± 0.052
6.235 ± 0.046
9.059 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.79 ± 0.031
3.733 ± 0.053
3.658 ± 0.037
3.0 ± 0.031
5.094 ± 0.057
5.503 ± 0.048
5.933 ± 0.057
7.733 ± 0.045
0.867 ± 0.022
3.665 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here