Acinetobacter phage ZZ1
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 256 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W0B451|W0B451_9CAUD Uncharacterized protein OS=Acinetobacter phage ZZ1 OX=1049283 GN=ZZ1p0140 PE=4 SV=1
MM1 pKa = 7.76 EE2 pKa = 5.54 NLIQPTEE9 pKa = 3.95 EE10 pKa = 3.74 QFEE13 pKa = 4.42 QIRR16 pKa = 11.84 DD17 pKa = 3.81 EE18 pKa = 5.7 LEE20 pKa = 4.19 GCSDD24 pKa = 4.16 DD25 pKa = 4.49 FGRR28 pKa = 11.84 THH30 pKa = 6.82 KK31 pKa = 10.59 RR32 pKa = 11.84 VLTEE36 pKa = 4.72 LGLDD40 pKa = 3.25 TDD42 pKa = 4.54 LRR44 pKa = 11.84 DD45 pKa = 3.59 SVIYY49 pKa = 10.8 NDD51 pKa = 3.66 TLEE54 pKa = 4.63 TYY56 pKa = 9.95 TFLCEE61 pKa = 6.07 NCDD64 pKa = 3.79 CWCSPDD70 pKa = 3.58 TRR72 pKa = 11.84 VHH74 pKa = 5.2 NQIAEE79 pKa = 4.09 MTVCEE84 pKa = 4.34 EE85 pKa = 4.57 CDD87 pKa = 3.44 EE88 pKa = 4.19 QLSS91 pKa = 3.76
Molecular weight: 10.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 3.948
IPC_protein 3.884
Toseland 3.694
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.617
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.139
Thurlkill 3.745
EMBOSS 3.77
Sillero 3.999
Patrickios 0.655
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.895
Protein with the highest isoelectric point:
>tr|I3WVL6|I3WVL6_9CAUD Uncharacterized protein OS=Acinetobacter phage ZZ1 OX=1049283 GN=ZZ1p0077 PE=4 SV=1
MM1 pKa = 7.68 NNLQTQLIIAARR13 pKa = 11.84 TNIKK17 pKa = 10.58 SVMKK21 pKa = 9.63 TMRR24 pKa = 11.84 PGPSIATVCITDD36 pKa = 3.4 RR37 pKa = 11.84 ADD39 pKa = 3.34 YY40 pKa = 11.29 DD41 pKa = 3.66 EE42 pKa = 5.49 LNRR45 pKa = 11.84 IQKK48 pKa = 9.92 DD49 pKa = 3.04 IKK51 pKa = 10.62 RR52 pKa = 11.84 LLNINTGRR60 pKa = 11.84 KK61 pKa = 8.67 KK62 pKa = 10.72
Molecular weight: 7.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.256
IPC2_protein 9.545
IPC_protein 9.78
Toseland 10.687
ProMoST 10.306
Dawson 10.76
Bjellqvist 10.365
Wikipedia 10.877
Rodwell 11.213
Grimsley 10.774
Solomon 10.833
Lehninger 10.818
Nozaki 10.657
DTASelect 10.365
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.672
Patrickios 10.994
IPC_peptide 10.847
IPC2_peptide 9.063
IPC2.peptide.svr19 8.607
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
256
0
256
51763
34
1303
202.2
22.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.051 ± 0.23
1.097 ± 0.074
6.194 ± 0.129
6.497 ± 0.21
4.281 ± 0.111
5.832 ± 0.238
1.936 ± 0.101
7.384 ± 0.142
7.364 ± 0.191
8.048 ± 0.143
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.687 ± 0.091
5.877 ± 0.111
3.632 ± 0.083
3.531 ± 0.109
4.148 ± 0.119
6.163 ± 0.151
6.335 ± 0.28
6.487 ± 0.117
1.343 ± 0.066
4.113 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here