Amino acid dipepetide frequency for Acinetobacter phage ZZ1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.965AlaAla: 4.965 ± 0.313
0.753AlaCys: 0.753 ± 0.136
4.212AlaAsp: 4.212 ± 0.271
4.791AlaGlu: 4.791 ± 0.353
2.724AlaPhe: 2.724 ± 0.196
4.675AlaGly: 4.675 ± 0.46
1.101AlaHis: 1.101 ± 0.16
5.409AlaIle: 5.409 ± 0.322
5.738AlaLys: 5.738 ± 0.321
5.68AlaLeu: 5.68 ± 0.349
1.642AlaMet: 1.642 ± 0.151
3.96AlaAsn: 3.96 ± 0.284
2.318AlaPro: 2.318 ± 0.272
2.782AlaGln: 2.782 ± 0.235
2.879AlaArg: 2.879 ± 0.216
4.096AlaSer: 4.096 ± 0.413
4.579AlaThr: 4.579 ± 0.573
4.617AlaVal: 4.617 ± 0.297
1.159AlaTrp: 1.159 ± 0.144
2.434AlaTyr: 2.434 ± 0.227
0.0AlaXaa: 0.0 ± 0.0
Cys
0.734CysAla: 0.734 ± 0.116
0.174CysCys: 0.174 ± 0.063
0.889CysAsp: 0.889 ± 0.138
0.811CysGlu: 0.811 ± 0.135
0.464CysPhe: 0.464 ± 0.101
0.657CysGly: 0.657 ± 0.114
0.309CysHis: 0.309 ± 0.071
0.753CysIle: 0.753 ± 0.112
0.773CysLys: 0.773 ± 0.116
0.676CysLeu: 0.676 ± 0.126
0.174CysMet: 0.174 ± 0.061
0.618CysAsn: 0.618 ± 0.109
0.618CysPro: 0.618 ± 0.132
0.251CysGln: 0.251 ± 0.071
0.483CysArg: 0.483 ± 0.103
0.695CysSer: 0.695 ± 0.127
0.85CysThr: 0.85 ± 0.135
0.638CysVal: 0.638 ± 0.124
0.135CysTrp: 0.135 ± 0.054
0.27CysTyr: 0.27 ± 0.07
0.0CysXaa: 0.0 ± 0.0
Asp
4.733AspAla: 4.733 ± 0.285
0.657AspCys: 0.657 ± 0.115
3.613AspAsp: 3.613 ± 0.356
4.347AspGlu: 4.347 ± 0.316
2.937AspPhe: 2.937 ± 0.242
4.038AspGly: 4.038 ± 0.265
1.14AspHis: 1.14 ± 0.155
4.521AspIle: 4.521 ± 0.294
4.038AspLys: 4.038 ± 0.288
5.139AspLeu: 5.139 ± 0.348
1.661AspMet: 1.661 ± 0.191
3.439AspAsn: 3.439 ± 0.233
2.26AspPro: 2.26 ± 0.241
1.816AspGln: 1.816 ± 0.204
2.376AspArg: 2.376 ± 0.234
3.477AspSer: 3.477 ± 0.273
3.613AspThr: 3.613 ± 0.284
3.864AspVal: 3.864 ± 0.29
1.101AspTrp: 1.101 ± 0.14
3.168AspTyr: 3.168 ± 0.273
0.0AspXaa: 0.0 ± 0.0
Glu
4.753GluAla: 4.753 ± 0.34
1.043GluCys: 1.043 ± 0.129
3.651GluAsp: 3.651 ± 0.314
3.845GluGlu: 3.845 ± 0.356
2.801GluPhe: 2.801 ± 0.262
3.072GluGly: 3.072 ± 0.248
1.507GluHis: 1.507 ± 0.181
5.371GluIle: 5.371 ± 0.35
3.999GluLys: 3.999 ± 0.294
6.588GluLeu: 6.588 ± 0.422
2.048GluMet: 2.048 ± 0.185
3.439GluAsn: 3.439 ± 0.245
1.932GluPro: 1.932 ± 0.23
2.144GluGln: 2.144 ± 0.208
2.55GluArg: 2.55 ± 0.251
3.845GluSer: 3.845 ± 0.3
3.902GluThr: 3.902 ± 0.289
4.385GluVal: 4.385 ± 0.264
0.657GluTrp: 0.657 ± 0.119
3.439GluTyr: 3.439 ± 0.312
0.0GluXaa: 0.0 ± 0.0
Phe
3.052PheAla: 3.052 ± 0.218
0.386PheCys: 0.386 ± 0.096
3.555PheAsp: 3.555 ± 0.234
3.246PheGlu: 3.246 ± 0.286
1.352PhePhe: 1.352 ± 0.173
2.917PheGly: 2.917 ± 0.287
0.406PheHis: 0.406 ± 0.086
3.13PheIle: 3.13 ± 0.241
3.96PheLys: 3.96 ± 0.289
2.879PheLeu: 2.879 ± 0.23
1.043PheMet: 1.043 ± 0.142
2.917PheAsn: 2.917 ± 0.192
1.101PhePro: 1.101 ± 0.125
1.178PheGln: 1.178 ± 0.142
1.565PheArg: 1.565 ± 0.179
2.666PheSer: 2.666 ± 0.24
2.55PheThr: 2.55 ± 0.231
2.917PheVal: 2.917 ± 0.227
0.522PheTrp: 0.522 ± 0.097
1.468PheTyr: 1.468 ± 0.203
0.0PheXaa: 0.0 ± 0.0
Gly
4.134GlyAla: 4.134 ± 0.441
0.599GlyCys: 0.599 ± 0.097
3.767GlyAsp: 3.767 ± 0.308
3.497GlyGlu: 3.497 ± 0.277
2.569GlyPhe: 2.569 ± 0.185
2.956GlyGly: 2.956 ± 0.406
0.966GlyHis: 0.966 ± 0.163
4.482GlyIle: 4.482 ± 0.397
3.941GlyLys: 3.941 ± 0.307
4.772GlyLeu: 4.772 ± 0.291
1.603GlyMet: 1.603 ± 0.21
2.898GlyAsn: 2.898 ± 0.271
1.758GlyPro: 1.758 ± 0.228
1.971GlyGln: 1.971 ± 0.167
2.299GlyArg: 2.299 ± 0.224
3.729GlySer: 3.729 ± 0.305
4.637GlyThr: 4.637 ± 0.498
3.96GlyVal: 3.96 ± 0.287
1.082GlyTrp: 1.082 ± 0.152
2.705GlyTyr: 2.705 ± 0.263
0.0GlyXaa: 0.0 ± 0.0
His
1.14HisAla: 1.14 ± 0.17
0.27HisCys: 0.27 ± 0.069
0.927HisAsp: 0.927 ± 0.155
1.14HisGlu: 1.14 ± 0.153
0.773HisPhe: 0.773 ± 0.143
1.14HisGly: 1.14 ± 0.166
0.425HisHis: 0.425 ± 0.098
1.739HisIle: 1.739 ± 0.215
1.661HisLys: 1.661 ± 0.19
1.43HisLeu: 1.43 ± 0.142
0.406HisMet: 0.406 ± 0.087
1.178HisAsn: 1.178 ± 0.171
1.159HisPro: 1.159 ± 0.154
0.502HisGln: 0.502 ± 0.102
0.869HisArg: 0.869 ± 0.11
0.908HisSer: 0.908 ± 0.118
1.236HisThr: 1.236 ± 0.155
1.391HisVal: 1.391 ± 0.162
0.232HisTrp: 0.232 ± 0.067
0.831HisTyr: 0.831 ± 0.131
0.0HisXaa: 0.0 ± 0.0
Ile
5.448IleAla: 5.448 ± 0.383
1.101IleCys: 1.101 ± 0.162
4.984IleAsp: 4.984 ± 0.31
5.854IleGlu: 5.854 ± 0.32
2.685IlePhe: 2.685 ± 0.234
3.825IleGly: 3.825 ± 0.325
1.256IleHis: 1.256 ± 0.147
5.042IleIle: 5.042 ± 0.325
6.607IleLys: 6.607 ± 0.393
5.429IleLeu: 5.429 ± 0.384
1.681IleMet: 1.681 ± 0.182
4.212IleAsn: 4.212 ± 0.31
2.531IlePro: 2.531 ± 0.221
3.246IleGln: 3.246 ± 0.269
3.188IleArg: 3.188 ± 0.274
4.559IleSer: 4.559 ± 0.328
4.753IleThr: 4.753 ± 0.281
4.25IleVal: 4.25 ± 0.261
0.657IleTrp: 0.657 ± 0.111
2.511IleTyr: 2.511 ± 0.218
0.0IleXaa: 0.0 ± 0.0
Lys
5.429LysAla: 5.429 ± 0.326
0.811LysCys: 0.811 ± 0.172
4.424LysAsp: 4.424 ± 0.334
4.463LysGlu: 4.463 ± 0.278
3.477LysPhe: 3.477 ± 0.295
4.096LysGly: 4.096 ± 0.287
1.855LysHis: 1.855 ± 0.221
4.965LysIle: 4.965 ± 0.31
4.27LysLys: 4.27 ± 0.329
6.588LysLeu: 6.588 ± 0.424
2.318LysMet: 2.318 ± 0.202
4.154LysAsn: 4.154 ± 0.29
2.685LysPro: 2.685 ± 0.239
2.376LysGln: 2.376 ± 0.224
3.362LysArg: 3.362 ± 0.235
4.656LysSer: 4.656 ± 0.38
4.501LysThr: 4.501 ± 0.292
4.868LysVal: 4.868 ± 0.328
1.043LysTrp: 1.043 ± 0.125
3.304LysTyr: 3.304 ± 0.24
0.0LysXaa: 0.0 ± 0.0
Leu
5.699LeuAla: 5.699 ± 0.357
0.966LeuCys: 0.966 ± 0.165
5.236LeuAsp: 5.236 ± 0.357
5.197LeuGlu: 5.197 ± 0.347
3.304LeuPhe: 3.304 ± 0.242
4.27LeuGly: 4.27 ± 0.36
1.7LeuHis: 1.7 ± 0.173
5.371LeuIle: 5.371 ± 0.34
6.607LeuLys: 6.607 ± 0.395
5.545LeuLeu: 5.545 ± 0.341
2.26LeuMet: 2.26 ± 0.216
5.487LeuAsn: 5.487 ± 0.353
3.13LeuPro: 3.13 ± 0.242
2.569LeuGln: 2.569 ± 0.204
3.439LeuArg: 3.439 ± 0.258
5.525LeuSer: 5.525 ± 0.339
5.255LeuThr: 5.255 ± 0.369
4.946LeuVal: 4.946 ± 0.309
0.811LeuTrp: 0.811 ± 0.134
3.168LeuTyr: 3.168 ± 0.228
0.0LeuXaa: 0.0 ± 0.0
Met
1.913MetAla: 1.913 ± 0.167
0.309MetCys: 0.309 ± 0.08
1.623MetAsp: 1.623 ± 0.167
1.43MetGlu: 1.43 ± 0.172
1.314MetPhe: 1.314 ± 0.185
1.063MetGly: 1.063 ± 0.13
0.56MetHis: 0.56 ± 0.109
1.777MetIle: 1.777 ± 0.192
2.685MetLys: 2.685 ± 0.23
2.067MetLeu: 2.067 ± 0.188
0.753MetMet: 0.753 ± 0.112
1.507MetAsn: 1.507 ± 0.167
0.869MetPro: 0.869 ± 0.128
1.159MetGln: 1.159 ± 0.155
1.178MetArg: 1.178 ± 0.153
2.202MetSer: 2.202 ± 0.206
1.816MetThr: 1.816 ± 0.187
1.294MetVal: 1.294 ± 0.163
0.27MetTrp: 0.27 ± 0.067
1.082MetTyr: 1.082 ± 0.142
0.0MetXaa: 0.0 ± 0.0
Asn
4.559AsnAla: 4.559 ± 0.318
0.464AsnCys: 0.464 ± 0.096
3.323AsnAsp: 3.323 ± 0.291
3.516AsnGlu: 3.516 ± 0.298
2.531AsnPhe: 2.531 ± 0.219
4.018AsnGly: 4.018 ± 0.344
1.024AsnHis: 1.024 ± 0.139
5.139AsnIle: 5.139 ± 0.335
3.767AsnLys: 3.767 ± 0.287
5.1AsnLeu: 5.1 ± 0.321
1.565AsnMet: 1.565 ± 0.172
3.304AsnAsn: 3.304 ± 0.339
2.473AsnPro: 2.473 ± 0.286
2.144AsnGln: 2.144 ± 0.209
2.415AsnArg: 2.415 ± 0.205
3.516AsnSer: 3.516 ± 0.214
3.593AsnThr: 3.593 ± 0.348
3.555AsnVal: 3.555 ± 0.348
0.773AsnTrp: 0.773 ± 0.117
1.99AsnTyr: 1.99 ± 0.21
0.0AsnXaa: 0.0 ± 0.0
Pro
2.763ProAla: 2.763 ± 0.284
0.251ProCys: 0.251 ± 0.075
2.396ProAsp: 2.396 ± 0.205
2.956ProGlu: 2.956 ± 0.271
1.758ProPhe: 1.758 ± 0.173
2.492ProGly: 2.492 ± 0.204
0.773ProHis: 0.773 ± 0.139
2.357ProIle: 2.357 ± 0.192
2.859ProLys: 2.859 ± 0.238
2.705ProLeu: 2.705 ± 0.27
0.444ProMet: 0.444 ± 0.091
2.318ProAsn: 2.318 ± 0.229
1.024ProPro: 1.024 ± 0.143
1.14ProGln: 1.14 ± 0.136
1.217ProArg: 1.217 ± 0.141
2.202ProSer: 2.202 ± 0.266
2.338ProThr: 2.338 ± 0.277
2.454ProVal: 2.454 ± 0.208
0.464ProTrp: 0.464 ± 0.084
1.41ProTyr: 1.41 ± 0.157
0.0ProXaa: 0.0 ± 0.0
Gln
2.84GlnAla: 2.84 ± 0.238
0.174GlnCys: 0.174 ± 0.06
1.661GlnAsp: 1.661 ± 0.217
2.222GlnGlu: 2.222 ± 0.189
1.603GlnPhe: 1.603 ± 0.18
1.893GlnGly: 1.893 ± 0.164
0.773GlnHis: 0.773 ± 0.151
2.531GlnIle: 2.531 ± 0.241
2.009GlnLys: 2.009 ± 0.191
3.593GlnLeu: 3.593 ± 0.295
1.352GlnMet: 1.352 ± 0.178
1.758GlnAsn: 1.758 ± 0.224
1.121GlnPro: 1.121 ± 0.157
1.391GlnGln: 1.391 ± 0.195
1.546GlnArg: 1.546 ± 0.144
2.164GlnSer: 2.164 ± 0.199
2.009GlnThr: 2.009 ± 0.207
2.434GlnVal: 2.434 ± 0.242
0.522GlnTrp: 0.522 ± 0.112
1.719GlnTyr: 1.719 ± 0.136
0.0GlnXaa: 0.0 ± 0.0
Arg
2.763ArgAla: 2.763 ± 0.268
0.406ArgCys: 0.406 ± 0.083
2.647ArgAsp: 2.647 ± 0.249
2.84ArgGlu: 2.84 ± 0.288
2.067ArgPhe: 2.067 ± 0.198
2.299ArgGly: 2.299 ± 0.186
0.831ArgHis: 0.831 ± 0.126
3.091ArgIle: 3.091 ± 0.213
3.304ArgLys: 3.304 ± 0.268
3.535ArgLeu: 3.535 ± 0.251
1.101ArgMet: 1.101 ± 0.146
2.299ArgAsn: 2.299 ± 0.22
1.178ArgPro: 1.178 ± 0.161
1.681ArgGln: 1.681 ± 0.186
1.739ArgArg: 1.739 ± 0.216
2.415ArgSer: 2.415 ± 0.241
2.357ArgThr: 2.357 ± 0.193
2.685ArgVal: 2.685 ± 0.247
0.715ArgTrp: 0.715 ± 0.123
1.526ArgTyr: 1.526 ± 0.196
0.0ArgXaa: 0.0 ± 0.0
Ser
3.729SerAla: 3.729 ± 0.277
0.425SerCys: 0.425 ± 0.099
4.115SerAsp: 4.115 ± 0.245
3.168SerGlu: 3.168 ± 0.286
2.724SerPhe: 2.724 ± 0.227
3.941SerGly: 3.941 ± 0.386
1.333SerHis: 1.333 ± 0.18
4.888SerIle: 4.888 ± 0.354
4.463SerLys: 4.463 ± 0.301
5.1SerLeu: 5.1 ± 0.313
1.661SerMet: 1.661 ± 0.186
4.076SerAsn: 4.076 ± 0.304
2.164SerPro: 2.164 ± 0.237
1.932SerGln: 1.932 ± 0.166
2.705SerArg: 2.705 ± 0.218
3.632SerSer: 3.632 ± 0.316
4.308SerThr: 4.308 ± 0.324
3.864SerVal: 3.864 ± 0.319
0.753SerTrp: 0.753 ± 0.126
2.647SerTyr: 2.647 ± 0.223
0.0SerXaa: 0.0 ± 0.0
Thr
4.231ThrAla: 4.231 ± 0.473
0.734ThrCys: 0.734 ± 0.102
3.304ThrAsp: 3.304 ± 0.269
4.212ThrGlu: 4.212 ± 0.308
2.956ThrPhe: 2.956 ± 0.247
4.154ThrGly: 4.154 ± 0.529
1.159ThrHis: 1.159 ± 0.157
4.965ThrIle: 4.965 ± 0.361
3.787ThrLys: 3.787 ± 0.238
5.004ThrLeu: 5.004 ± 0.347
1.642ThrMet: 1.642 ± 0.18
3.69ThrAsn: 3.69 ± 0.297
3.188ThrPro: 3.188 ± 0.282
2.473ThrGln: 2.473 ± 0.254
2.338ThrArg: 2.338 ± 0.222
3.671ThrSer: 3.671 ± 0.31
3.748ThrThr: 3.748 ± 0.387
4.675ThrVal: 4.675 ± 0.402
0.869ThrTrp: 0.869 ± 0.143
2.55ThrTyr: 2.55 ± 0.248
0.0ThrXaa: 0.0 ± 0.0
Val
4.25ValAla: 4.25 ± 0.286
0.889ValCys: 0.889 ± 0.142
3.767ValAsp: 3.767 ± 0.257
4.192ValGlu: 4.192 ± 0.301
2.569ValPhe: 2.569 ± 0.213
3.477ValGly: 3.477 ± 0.274
1.236ValHis: 1.236 ± 0.14
4.405ValIle: 4.405 ± 0.303
5.564ValLys: 5.564 ± 0.325
4.714ValLeu: 4.714 ± 0.277
1.739ValMet: 1.739 ± 0.162
4.096ValAsn: 4.096 ± 0.315
2.589ValPro: 2.589 ± 0.213
2.608ValGln: 2.608 ± 0.202
2.801ValArg: 2.801 ± 0.26
4.192ValSer: 4.192 ± 0.279
3.941ValThr: 3.941 ± 0.306
4.154ValVal: 4.154 ± 0.259
0.734ValTrp: 0.734 ± 0.115
2.956ValTyr: 2.956 ± 0.205
0.0ValXaa: 0.0 ± 0.0
Trp
0.734TrpAla: 0.734 ± 0.122
0.155TrpCys: 0.155 ± 0.053
0.927TrpAsp: 0.927 ± 0.161
0.831TrpGlu: 0.831 ± 0.125
0.522TrpPhe: 0.522 ± 0.094
0.541TrpGly: 0.541 ± 0.11
0.328TrpHis: 0.328 ± 0.08
0.927TrpIle: 0.927 ± 0.141
0.831TrpLys: 0.831 ± 0.152
0.966TrpLeu: 0.966 ± 0.12
0.58TrpMet: 0.58 ± 0.1
0.638TrpAsn: 0.638 ± 0.112
0.522TrpPro: 0.522 ± 0.092
0.386TrpGln: 0.386 ± 0.088
0.618TrpArg: 0.618 ± 0.118
1.101TrpSer: 1.101 ± 0.146
0.908TrpThr: 0.908 ± 0.133
0.927TrpVal: 0.927 ± 0.138
0.328TrpTrp: 0.328 ± 0.081
0.657TrpTyr: 0.657 ± 0.109
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.647TyrAla: 2.647 ± 0.206
0.406TyrCys: 0.406 ± 0.087
2.917TyrAsp: 2.917 ± 0.293
2.415TyrGlu: 2.415 ± 0.253
1.681TyrPhe: 1.681 ± 0.182
2.801TyrGly: 2.801 ± 0.236
0.676TyrHis: 0.676 ± 0.109
2.975TyrIle: 2.975 ± 0.294
2.782TyrLys: 2.782 ± 0.256
2.975TyrLeu: 2.975 ± 0.259
1.178TyrMet: 1.178 ± 0.138
2.782TyrAsn: 2.782 ± 0.236
1.7TyrPro: 1.7 ± 0.155
1.565TyrGln: 1.565 ± 0.146
1.913TyrArg: 1.913 ± 0.185
2.376TyrSer: 2.376 ± 0.24
2.454TyrThr: 2.454 ± 0.261
2.994TyrVal: 2.994 ± 0.293
0.599TyrTrp: 0.599 ± 0.076
1.294TyrTyr: 1.294 ± 0.193
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 256 proteins (51763 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski