Pluralibacter gergoviae (Enterobacter gergoviae)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Pluralibacter

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4977 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F0VKL5|A0A0F0VKL5_PLUGE LysR family transcriptional regulator OS=Pluralibacter gergoviae OX=61647 GN=ABW06_05510 PE=3 SV=1
MM1 pKa = 7.4LAGMTSSEE9 pKa = 4.2LGDD12 pKa = 2.82WHH14 pKa = 6.75LFYY17 pKa = 10.89RR18 pKa = 11.84EE19 pKa = 5.11RR20 pKa = 11.84YY21 pKa = 8.48FQDD24 pKa = 3.16AQLDD28 pKa = 3.72AHH30 pKa = 6.73FSSLLYY36 pKa = 10.21TISTFLYY43 pKa = 9.09QDD45 pKa = 3.7PDD47 pKa = 3.09ITPADD52 pKa = 4.09FSLLSPSAGAGADD65 pKa = 3.55NEE67 pKa = 4.15QDD69 pKa = 3.62DD70 pKa = 4.7DD71 pKa = 5.45AMMLAAEE78 pKa = 5.8GITGGTRR85 pKa = 11.84YY86 pKa = 10.33GPADD90 pKa = 3.17

Molecular weight: 9.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J5L8B7|A0A0J5L8B7_PLUGE Uncharacterized protein OS=Pluralibacter gergoviae OX=61647 GN=ABW06_09220 PE=4 SV=1
MM1 pKa = 7.57NLPFLRR7 pKa = 11.84SLRR10 pKa = 11.84RR11 pKa = 11.84DD12 pKa = 3.23RR13 pKa = 11.84FLHH16 pKa = 6.18LLIATGIILSFFVPFRR32 pKa = 11.84PRR34 pKa = 11.84GWPASIDD41 pKa = 2.72WHH43 pKa = 6.29TIVTLSGLMLLTKK56 pKa = 9.99GVEE59 pKa = 3.66MSGYY63 pKa = 10.28FDD65 pKa = 3.31VLGRR69 pKa = 11.84KK70 pKa = 6.13MARR73 pKa = 11.84RR74 pKa = 11.84FATEE78 pKa = 3.19RR79 pKa = 11.84RR80 pKa = 11.84LALFMVLAAALLSTFLTNDD99 pKa = 2.64VALFIVVPLTLTLKK113 pKa = 9.89KK114 pKa = 9.46WCALPVSRR122 pKa = 11.84LIIFEE127 pKa = 4.24ALAVNAGSLLTPIGNPQNILLWGSSGLSFFNFSLQMVPLALAMMLTLLALCRR179 pKa = 11.84CCFSGAALRR188 pKa = 11.84FQSGDD193 pKa = 3.44ALPVWRR199 pKa = 11.84PKK201 pKa = 10.67LVWSCLGLYY210 pKa = 10.5LIFLAALEE218 pKa = 4.27MRR220 pKa = 11.84LEE222 pKa = 4.17LWGLAAVAAGFLLLEE237 pKa = 4.11RR238 pKa = 11.84QILVRR243 pKa = 11.84VDD245 pKa = 2.96WTLLLVFMAMFIDD258 pKa = 3.91VHH260 pKa = 7.9LLTQLPALHH269 pKa = 6.69HH270 pKa = 6.71ALAQVGLLSDD280 pKa = 3.93GGLWLTAIGLSQVISNVPATILLLNAVPPSVLLAWAVNVGGFGLLPGSLANLIALRR336 pKa = 11.84MAGDD340 pKa = 3.39RR341 pKa = 11.84RR342 pKa = 11.84IWWRR346 pKa = 11.84FHH348 pKa = 6.29LFSVPMLLWAMLAGFGLYY366 pKa = 10.7LLIHH370 pKa = 6.95

Molecular weight: 40.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4977

0

4977

1595228

29

10855

320.5

35.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.739 ± 0.046

1.083 ± 0.014

5.276 ± 0.033

5.454 ± 0.03

3.732 ± 0.03

7.859 ± 0.041

2.174 ± 0.018

5.497 ± 0.032

3.843 ± 0.033

10.906 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.022

3.384 ± 0.031

4.606 ± 0.027

4.248 ± 0.03

6.203 ± 0.039

5.852 ± 0.03

5.178 ± 0.047

7.032 ± 0.037

1.556 ± 0.015

2.698 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski