Caldimicrobium thiodismutans

Taxonomy: cellular organisms; Bacteria; Thermodesulfobacteria; Thermodesulfobacteria; Thermodesulfobacteriales; Thermodesulfobacteriaceae; Caldimicrobium

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U5ALK2|A0A0U5ALK2_9BACT Uncharacterized protein OS=Caldimicrobium thiodismutans OX=1653476 GN=THC_0324 PE=4 SV=1
MM1 pKa = 7.62LMATLVVDD9 pKa = 4.71DD10 pKa = 5.21EE11 pKa = 4.73LCIGCGTCVEE21 pKa = 4.62LCPNVFEE28 pKa = 5.6LGSDD32 pKa = 3.12EE33 pKa = 4.08KK34 pKa = 11.35AYY36 pKa = 11.07VKK38 pKa = 10.6NQEE41 pKa = 3.81GCKK44 pKa = 9.96EE45 pKa = 4.3CNCEE49 pKa = 3.91EE50 pKa = 5.08AINSCPVSAISWQDD64 pKa = 2.83

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U5AXT9|A0A0U5AXT9_9BACT Probable membrane transporter protein OS=Caldimicrobium thiodismutans OX=1653476 GN=THC_0878 PE=3 SV=1
MM1 pKa = 8.03PKK3 pKa = 8.96RR4 pKa = 11.84TYY6 pKa = 10.1QPSRR10 pKa = 11.84LKK12 pKa = 9.72MKK14 pKa = 9.96RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.92GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 9.94SGRR29 pKa = 11.84RR30 pKa = 11.84ILKK33 pKa = 8.48NRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.16GRR40 pKa = 11.84YY41 pKa = 8.76RR42 pKa = 11.84LTVV45 pKa = 2.95

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1771

0

1771

553420

40

1744

312.5

35.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.087 ± 0.053

1.013 ± 0.023

4.085 ± 0.037

8.91 ± 0.068

5.606 ± 0.064

6.616 ± 0.048

1.565 ± 0.022

7.696 ± 0.053

8.78 ± 0.053

12.464 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.727 ± 0.021

3.316 ± 0.035

4.45 ± 0.034

2.615 ± 0.026

4.802 ± 0.042

5.431 ± 0.036

4.267 ± 0.034

5.859 ± 0.05

0.946 ± 0.018

3.768 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski