Proteobacteria bacterium 228

Taxonomy: cellular organisms; Bacteria; Proteobacteria; unclassified Proteobacteria

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5549 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S5KJE5|A0A2S5KJE5_9PROT Uncharacterized protein OS=Proteobacteria bacterium 228 OX=2083153 GN=C4K68_23160 PE=3 SV=1
DDD2 pKa = 4.83LSQMVDDD9 pKa = 2.58AEEE12 pKa = 3.97HHH14 pKa = 5.97GYYY17 pKa = 10.9DD18 pKa = 3.45IHHH21 pKa = 5.84LSHHH25 pKa = 6.15SEEE28 pKa = 4.41EEE30 pKa = 4.14QLGSFTLDDD39 pKa = 2.83TTAEEE44 pKa = 4.19RR45 pKa = 11.84ADDD48 pKa = 3.96DD49 pKa = 4.36AKKK52 pKa = 10.82GAALTDDD59 pKa = 3.6GDDD62 pKa = 3.55LLYYY66 pKa = 10.18CEEE69 pKa = 3.97AQDDD73 pKa = 3.67GQSFVSLLAQLT

Molecular weight: 8.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S5KUN2|A0A2S5KUN2_9PROT CoA transferase OS=Proteobacteria bacterium 228 OX=2083153 GN=C4K68_04685 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34NGQKK29 pKa = 10.07VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.73RR41 pKa = 11.84LCAA44 pKa = 3.9

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5549

0

5549

1848317

29

5247

333.1

36.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.331 ± 0.041

1.076 ± 0.01

5.324 ± 0.028

5.562 ± 0.035

3.585 ± 0.02

7.387 ± 0.038

2.451 ± 0.018

5.206 ± 0.027

3.42 ± 0.03

11.538 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.016

3.104 ± 0.027

4.523 ± 0.027

5.265 ± 0.041

5.869 ± 0.036

6.423 ± 0.041

5.093 ± 0.046

7.057 ± 0.027

1.439 ± 0.017

2.728 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski