Rhodobacteraceae bacterium CH30

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N9QKY4|A0A3N9QKY4_9RHOB Uncharacterized protein OS=Rhodobacteraceae bacterium CH30 OX=2490538 GN=EHS17_07135 PE=4 SV=1
MM1 pKa = 7.21TATTEE6 pKa = 4.1MPSPINFTDD15 pKa = 3.66SACAKK20 pKa = 10.13VRR22 pKa = 11.84DD23 pKa = 4.02LVAEE27 pKa = 4.25EE28 pKa = 4.83GNPEE32 pKa = 3.75LKK34 pKa = 10.69LRR36 pKa = 11.84VFVTGGGCSGFQYY49 pKa = 10.94GFTFDD54 pKa = 5.2EE55 pKa = 4.78IVNDD59 pKa = 4.08DD60 pKa = 3.52DD61 pKa = 4.17TAIEE65 pKa = 4.04RR66 pKa = 11.84DD67 pKa = 3.55GVTFLVDD74 pKa = 3.48PMSYY78 pKa = 10.19QYY80 pKa = 11.57LVGAEE85 pKa = 4.34IDD87 pKa = 3.84YY88 pKa = 11.09QDD90 pKa = 4.21NLEE93 pKa = 4.25GSQFVIRR100 pKa = 11.84NPNATTTCGCGSSFTVV116 pKa = 3.3

Molecular weight: 12.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N9QFR1|A0A3N9QFR1_9RHOB Glutamate-1-semialdehyde 2 1-aminomutase OS=Rhodobacteraceae bacterium CH30 OX=2490538 GN=hemL PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.29QPSNTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.05RR14 pKa = 11.84THH16 pKa = 6.09GFLVRR21 pKa = 11.84NKK23 pKa = 8.78TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 8.68RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3115

0

3115

983925

18

1655

315.9

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.201 ± 0.053

1.066 ± 0.014

5.167 ± 0.033

5.451 ± 0.038

3.637 ± 0.029

8.088 ± 0.048

2.237 ± 0.022

4.889 ± 0.034

3.789 ± 0.037

11.525 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.679 ± 0.021

2.856 ± 0.029

4.66 ± 0.031

4.055 ± 0.033

6.094 ± 0.04

5.751 ± 0.028

4.798 ± 0.03

7.192 ± 0.037

1.388 ± 0.021

2.478 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski