Amino acid dipepetide frequency for Rhodobacteraceae bacterium CH30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.334AlaAla: 15.334 ± 0.199	1.594AlaCys: 1.594 ± 0.047	6.302AlaAsp: 6.302 ± 0.084	6.876AlaGlu: 6.876 ± 0.078	4.046AlaPhe: 4.046 ± 0.062	10.661AlaGly: 10.661 ± 0.125	2.509AlaHis: 2.509 ± 0.054	6.071AlaIle: 6.071 ± 0.093	4.024AlaLys: 4.024 ± 0.072	15.415AlaLeu: 15.415 ± 0.185	3.561AlaMet: 3.561 ± 0.055	2.902AlaAsn: 2.902 ± 0.056	4.842AlaPro: 4.842 ± 0.086	5.176AlaGln: 5.176 ± 0.077	8.363AlaArg: 8.363 ± 0.114	6.969AlaSer: 6.969 ± 0.105	5.1AlaThr: 5.1 ± 0.073	7.803AlaVal: 7.803 ± 0.095	1.757AlaTrp: 1.757 ± 0.048	2.708AlaTyr: 2.708 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	1.217CysAla: 1.217 ± 0.037	0.168CysCys: 0.168 ± 0.013	0.635CysAsp: 0.635 ± 0.027	0.567CysGlu: 0.567 ± 0.025	0.335CysPhe: 0.335 ± 0.021	1.094CysGly: 1.094 ± 0.034	0.33CysHis: 0.33 ± 0.02	0.446CysIle: 0.446 ± 0.023	0.287CysLys: 0.287 ± 0.017	1.097CysLeu: 1.097 ± 0.041	0.245CysMet: 0.245 ± 0.015	0.305CysAsn: 0.305 ± 0.018	0.584CysPro: 0.584 ± 0.025	0.364CysGln: 0.364 ± 0.018	0.701CysArg: 0.701 ± 0.03	0.615CysSer: 0.615 ± 0.023	0.465CysThr: 0.465 ± 0.024	0.748CysVal: 0.748 ± 0.028	0.172CysTrp: 0.172 ± 0.02	0.289CysTyr: 0.289 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	6.756AspAla: 6.756 ± 0.086	0.527AspCys: 0.527 ± 0.024	2.51AspAsp: 2.51 ± 0.059	3.341AspGlu: 3.341 ± 0.071	2.125AspPhe: 2.125 ± 0.06	4.477AspGly: 4.477 ± 0.072	0.951AspHis: 0.951 ± 0.028	2.843AspIle: 2.843 ± 0.062	2.343AspLys: 2.343 ± 0.06	4.681AspLeu: 4.681 ± 0.071	1.415AspMet: 1.415 ± 0.037	1.502AspAsn: 1.502 ± 0.046	2.375AspPro: 2.375 ± 0.05	1.477AspGln: 1.477 ± 0.039	2.432AspArg: 2.432 ± 0.055	2.845AspSer: 2.845 ± 0.058	2.641AspThr: 2.641 ± 0.05	3.761AspVal: 3.761 ± 0.065	0.947AspTrp: 0.947 ± 0.033	1.716AspTyr: 1.716 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	6.854GluAla: 6.854 ± 0.11	0.451GluCys: 0.451 ± 0.024	2.292GluAsp: 2.292 ± 0.056	2.782GluGlu: 2.782 ± 0.069	1.746GluPhe: 1.746 ± 0.043	3.778GluGly: 3.778 ± 0.059	1.393GluHis: 1.393 ± 0.044	3.062GluIle: 3.062 ± 0.061	2.264GluLys: 2.264 ± 0.058	6.359GluLeu: 6.359 ± 0.087	1.527GluMet: 1.527 ± 0.04	1.609GluAsn: 1.609 ± 0.045	2.16GluPro: 2.16 ± 0.053	2.792GluGln: 2.792 ± 0.063	3.945GluArg: 3.945 ± 0.077	2.558GluSer: 2.558 ± 0.052	2.744GluThr: 2.744 ± 0.06	4.298GluVal: 4.298 ± 0.074	0.693GluTrp: 0.693 ± 0.027	1.201GluTyr: 1.201 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	4.674PheAla: 4.674 ± 0.077	0.44PheCys: 0.44 ± 0.023	2.439PheAsp: 2.439 ± 0.055	1.977PheGlu: 1.977 ± 0.045	1.464PhePhe: 1.464 ± 0.045	3.292PheGly: 3.292 ± 0.067	0.743PheHis: 0.743 ± 0.032	1.907PheIle: 1.907 ± 0.047	1.252PheLys: 1.252 ± 0.035	3.096PheLeu: 3.096 ± 0.074	0.965PheMet: 0.965 ± 0.031	1.33PheAsn: 1.33 ± 0.037	1.435PhePro: 1.435 ± 0.035	0.909PheGln: 0.909 ± 0.035	1.677PheArg: 1.677 ± 0.047	2.608PheSer: 2.608 ± 0.063	1.877PheThr: 1.877 ± 0.046	2.721PheVal: 2.721 ± 0.057	0.522PheTrp: 0.522 ± 0.026	1.043PheTyr: 1.043 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	7.964GlyAla: 7.964 ± 0.101	0.977GlyCys: 0.977 ± 0.036	3.874GlyAsp: 3.874 ± 0.063	5.029GlyGlu: 5.029 ± 0.076	3.474GlyPhe: 3.474 ± 0.07	6.628GlyGly: 6.628 ± 0.099	1.915GlyHis: 1.915 ± 0.05	4.409GlyIle: 4.409 ± 0.077	4.138GlyLys: 4.138 ± 0.082	8.612GlyLeu: 8.612 ± 0.109	2.578GlyMet: 2.578 ± 0.054	2.398GlyAsn: 2.398 ± 0.05	2.203GlyPro: 2.203 ± 0.047	3.357GlyGln: 3.357 ± 0.064	4.948GlyArg: 4.948 ± 0.08	4.626GlySer: 4.626 ± 0.075	3.649GlyThr: 3.649 ± 0.069	6.396GlyVal: 6.396 ± 0.093	1.313GlyTrp: 1.313 ± 0.038	2.388GlyTyr: 2.388 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	2.686HisAla: 2.686 ± 0.049	0.301HisCys: 0.301 ± 0.017	1.221HisAsp: 1.221 ± 0.035	1.193HisGlu: 1.193 ± 0.036	0.976HisPhe: 0.976 ± 0.034	1.945HisGly: 1.945 ± 0.053	0.648HisHis: 0.648 ± 0.032	1.214HisIle: 1.214 ± 0.036	0.71HisLys: 0.71 ± 0.029	2.223HisLeu: 2.223 ± 0.049	0.479HisMet: 0.479 ± 0.023	0.616HisAsn: 0.616 ± 0.026	1.426HisPro: 1.426 ± 0.039	0.862HisGln: 0.862 ± 0.029	1.082HisArg: 1.082 ± 0.036	1.163HisSer: 1.163 ± 0.032	1.056HisThr: 1.056 ± 0.034	1.344HisVal: 1.344 ± 0.043	0.419HisTrp: 0.419 ± 0.023	0.806HisTyr: 0.806 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	6.626IleAla: 6.626 ± 0.094	0.521IleCys: 0.521 ± 0.025	3.071IleAsp: 3.071 ± 0.06	3.195IleGlu: 3.195 ± 0.063	1.402IlePhe: 1.402 ± 0.039	4.482IleGly: 4.482 ± 0.073	1.049IleHis: 1.049 ± 0.034	2.226IleIle: 2.226 ± 0.058	2.007IleLys: 2.007 ± 0.053	4.184IleLeu: 4.184 ± 0.083	0.982IleMet: 0.982 ± 0.031	1.738IleAsn: 1.738 ± 0.04	2.227IlePro: 2.227 ± 0.047	1.393IleGln: 1.393 ± 0.036	2.984IleArg: 2.984 ± 0.063	3.053IleSer: 3.053 ± 0.054	2.57IleThr: 2.57 ± 0.055	3.556IleVal: 3.556 ± 0.058	0.497IleTrp: 0.497 ± 0.026	1.122IleTyr: 1.122 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	4.277LysAla: 4.277 ± 0.081	0.21LysCys: 0.21 ± 0.015	1.821LysAsp: 1.821 ± 0.048	1.744LysGlu: 1.744 ± 0.05	1.005LysPhe: 1.005 ± 0.035	2.825LysGly: 2.825 ± 0.054	0.816LysHis: 0.816 ± 0.031	1.946LysIle: 1.946 ± 0.048	1.86LysLys: 1.86 ± 0.058	4.509LysLeu: 4.509 ± 0.082	1.031LysMet: 1.031 ± 0.036	1.316LysAsn: 1.316 ± 0.045	2.249LysPro: 2.249 ± 0.057	1.603LysGln: 1.603 ± 0.047	2.326LysArg: 2.326 ± 0.049	2.081LysSer: 2.081 ± 0.049	2.221LysThr: 2.221 ± 0.055	2.899LysVal: 2.899 ± 0.062	0.373LysTrp: 0.373 ± 0.021	0.775LysTyr: 0.775 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	16.627LeuAla: 16.627 ± 0.181	1.2LeuCys: 1.2 ± 0.041	6.102LeuAsp: 6.102 ± 0.087	5.536LeuGlu: 5.536 ± 0.082	4.158LeuPhe: 4.158 ± 0.075	8.679LeuGly: 8.679 ± 0.101	2.087LeuHis: 2.087 ± 0.044	5.059LeuIle: 5.059 ± 0.069	4.544LeuLys: 4.544 ± 0.084	13.348LeuLeu: 13.348 ± 0.182	2.975LeuMet: 2.975 ± 0.062	3.111LeuAsn: 3.111 ± 0.053	6.553LeuPro: 6.553 ± 0.107	4.165LeuGln: 4.165 ± 0.083	6.14LeuArg: 6.14 ± 0.093	7.754LeuSer: 7.754 ± 0.1	5.848LeuThr: 5.848 ± 0.084	7.513LeuVal: 7.513 ± 0.1	1.438LeuTrp: 1.438 ± 0.045	2.412LeuTyr: 2.412 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	3.382MetAla: 3.382 ± 0.062	0.177MetCys: 0.177 ± 0.013	1.258MetAsp: 1.258 ± 0.035	1.127MetGlu: 1.127 ± 0.033	0.767MetPhe: 0.767 ± 0.031	2.068MetGly: 2.068 ± 0.047	0.595MetHis: 0.595 ± 0.026	1.122MetIle: 1.122 ± 0.04	1.192MetLys: 1.192 ± 0.032	3.347MetLeu: 3.347 ± 0.062	0.776MetMet: 0.776 ± 0.032	0.863MetAsn: 0.863 ± 0.03	1.474MetPro: 1.474 ± 0.039	1.305MetGln: 1.305 ± 0.034	1.631MetArg: 1.631 ± 0.04	1.702MetSer: 1.702 ± 0.045	1.415MetThr: 1.415 ± 0.036	1.934MetVal: 1.934 ± 0.047	0.227MetTrp: 0.227 ± 0.015	0.43MetTyr: 0.43 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.221AsnAla: 3.221 ± 0.057	0.304AsnCys: 0.304 ± 0.019	1.368AsnAsp: 1.368 ± 0.045	1.441AsnGlu: 1.441 ± 0.035	0.999AsnPhe: 0.999 ± 0.033	2.406AsnGly: 2.406 ± 0.056	0.608AsnHis: 0.608 ± 0.026	1.541AsnIle: 1.541 ± 0.041	1.077AsnLys: 1.077 ± 0.036	2.975AsnLeu: 2.975 ± 0.055	0.763AsnMet: 0.763 ± 0.031	0.935AsnAsn: 0.935 ± 0.037	1.926AsnPro: 1.926 ± 0.051	1.076AsnGln: 1.076 ± 0.038	1.74AsnArg: 1.74 ± 0.044	1.507AsnSer: 1.507 ± 0.042	1.576AsnThr: 1.576 ± 0.047	1.904AsnVal: 1.904 ± 0.044	0.465AsnTrp: 0.465 ± 0.019	0.727AsnTyr: 0.727 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	6.298ProAla: 6.298 ± 0.106	0.449ProCys: 0.449 ± 0.022	3.052ProAsp: 3.052 ± 0.061	3.161ProGlu: 3.161 ± 0.053	1.805ProPhe: 1.805 ± 0.043	3.695ProGly: 3.695 ± 0.068	1.033ProHis: 1.033 ± 0.035	1.734ProIle: 1.734 ± 0.046	1.571ProLys: 1.571 ± 0.05	5.464ProLeu: 5.464 ± 0.09	1.18ProMet: 1.18 ± 0.037	1.145ProAsn: 1.145 ± 0.036	1.911ProPro: 1.911 ± 0.058	1.968ProGln: 1.968 ± 0.053	2.2ProArg: 2.2 ± 0.053	2.26ProSer: 2.26 ± 0.046	1.86ProThr: 1.86 ± 0.044	3.907ProVal: 3.907 ± 0.066	0.696ProTrp: 0.696 ± 0.031	1.207ProTyr: 1.207 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	5.568GlnAla: 5.568 ± 0.092	0.374GlnCys: 0.374 ± 0.02	1.858GlnAsp: 1.858 ± 0.044	1.638GlnGlu: 1.638 ± 0.043	1.154GlnPhe: 1.154 ± 0.032	3.272GlnGly: 3.272 ± 0.061	1.024GlnHis: 1.024 ± 0.032	1.913GlnIle: 1.913 ± 0.042	1.399GlnLys: 1.399 ± 0.043	4.764GlnLeu: 4.764 ± 0.08	1.08GlnMet: 1.08 ± 0.034	1.086GlnAsn: 1.086 ± 0.031	1.823GlnPro: 1.823 ± 0.045	2.498GlnGln: 2.498 ± 0.067	2.746GlnArg: 2.746 ± 0.054	2.258GlnSer: 2.258 ± 0.047	1.921GlnThr: 1.921 ± 0.047	2.75GlnVal: 2.75 ± 0.051	0.574GlnTrp: 0.574 ± 0.026	0.85GlnTyr: 0.85 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	6.493ArgAla: 6.493 ± 0.093	0.575ArgCys: 0.575 ± 0.028	3.177ArgAsp: 3.177 ± 0.063	4.07ArgGlu: 4.07 ± 0.068	2.69ArgPhe: 2.69 ± 0.061	3.552ArgGly: 3.552 ± 0.058	1.82ArgHis: 1.82 ± 0.045	3.311ArgIle: 3.311 ± 0.053	2.077ArgLys: 2.077 ± 0.054	7.624ArgLeu: 7.624 ± 0.104	1.742ArgMet: 1.742 ± 0.039	1.652ArgAsn: 1.652 ± 0.047	2.452ArgPro: 2.452 ± 0.053	2.939ArgGln: 2.939 ± 0.065	3.893ArgArg: 3.893 ± 0.086	2.908ArgSer: 2.908 ± 0.054	2.556ArgThr: 2.556 ± 0.055	4.474ArgVal: 4.474 ± 0.072	0.892ArgTrp: 0.892 ± 0.03	2.044ArgTyr: 2.044 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	6.608SerAla: 6.608 ± 0.095	0.619SerCys: 0.619 ± 0.029	2.86SerAsp: 2.86 ± 0.052	3.044SerGlu: 3.044 ± 0.057	2.238SerPhe: 2.238 ± 0.053	5.608SerGly: 5.608 ± 0.086	1.39SerHis: 1.39 ± 0.04	2.657SerIle: 2.657 ± 0.062	1.973SerLys: 1.973 ± 0.05	6.537SerLeu: 6.537 ± 0.084	1.5SerMet: 1.5 ± 0.042	1.611SerAsn: 1.611 ± 0.043	2.646SerPro: 2.646 ± 0.053	2.124SerGln: 2.124 ± 0.051	3.569SerArg: 3.569 ± 0.061	3.385SerSer: 3.385 ± 0.078	2.801SerThr: 2.801 ± 0.052	4.059SerVal: 4.059 ± 0.074	0.818SerTrp: 0.818 ± 0.028	1.467SerTyr: 1.467 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	4.867ThrAla: 4.867 ± 0.069	0.445ThrCys: 0.445 ± 0.022	2.3ThrAsp: 2.3 ± 0.052	2.266ThrGlu: 2.266 ± 0.053	1.636ThrPhe: 1.636 ± 0.044	4.245ThrGly: 4.245 ± 0.074	1.062ThrHis: 1.062 ± 0.029	2.203ThrIle: 2.203 ± 0.047	1.106ThrLys: 1.106 ± 0.04	7.316ThrLeu: 7.316 ± 0.11	1.062ThrMet: 1.062 ± 0.034	1.118ThrAsn: 1.118 ± 0.034	3.155ThrPro: 3.155 ± 0.055	1.921ThrGln: 1.921 ± 0.041	3.33ThrArg: 3.33 ± 0.057	2.5ThrSer: 2.5 ± 0.051	2.396ThrThr: 2.396 ± 0.055	3.616ThrVal: 3.616 ± 0.063	0.486ThrTrp: 0.486 ± 0.022	0.946ThrTyr: 0.946 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	8.522ValAla: 8.522 ± 0.107	0.866ValCys: 0.866 ± 0.028	3.747ValAsp: 3.747 ± 0.069	3.998ValGlu: 3.998 ± 0.072	2.744ValPhe: 2.744 ± 0.053	5.081ValGly: 5.081 ± 0.077	1.395ValHis: 1.395 ± 0.035	3.676ValIle: 3.676 ± 0.068	2.843ValLys: 2.843 ± 0.066	8.415ValLeu: 8.415 ± 0.122	2.011ValMet: 2.011 ± 0.043	2.276ValAsn: 2.276 ± 0.05	3.137ValPro: 3.137 ± 0.053	2.3ValGln: 2.3 ± 0.055	4.311ValArg: 4.311 ± 0.07	4.655ValSer: 4.655 ± 0.075	3.512ValThr: 3.512 ± 0.064	5.744ValVal: 5.744 ± 0.094	0.979ValTrp: 0.979 ± 0.034	1.703ValTyr: 1.703 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	1.243TrpAla: 1.243 ± 0.037	0.18TrpCys: 0.18 ± 0.015	0.55TrpAsp: 0.55 ± 0.023	0.465TrpGlu: 0.465 ± 0.023	0.579TrpPhe: 0.579 ± 0.026	0.932TrpGly: 0.932 ± 0.035	0.412TrpHis: 0.412 ± 0.021	0.585TrpIle: 0.585 ± 0.026	0.458TrpLys: 0.458 ± 0.022	2.248TrpLeu: 2.248 ± 0.06	0.417TrpMet: 0.417 ± 0.021	0.34TrpAsn: 0.34 ± 0.018	0.652TrpPro: 0.652 ± 0.027	1.086TrpGln: 1.086 ± 0.036	1.08TrpArg: 1.08 ± 0.034	0.666TrpSer: 0.666 ± 0.028	0.521TrpThr: 0.521 ± 0.022	0.963TrpVal: 0.963 ± 0.034	0.238TrpTrp: 0.238 ± 0.017	0.264TrpTyr: 0.264 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.796TyrAla: 2.796 ± 0.058	0.285TyrCys: 0.285 ± 0.018	1.227TyrAsp: 1.227 ± 0.036	1.058TyrGlu: 1.058 ± 0.033	1.027TyrPhe: 1.027 ± 0.032	2.156TyrGly: 2.156 ± 0.044	0.587TyrHis: 0.587 ± 0.022	0.961TyrIle: 0.961 ± 0.033	0.763TyrLys: 0.763 ± 0.027	3.035TyrLeu: 3.035 ± 0.058	0.503TyrMet: 0.503 ± 0.023	0.706TyrAsn: 0.706 ± 0.025	1.366TyrPro: 1.366 ± 0.039	1.236TyrGln: 1.236 ± 0.037	1.843TyrArg: 1.843 ± 0.048	1.401TyrSer: 1.401 ± 0.039	1.246TyrThr: 1.246 ± 0.038	1.527TyrVal: 1.527 ± 0.032	0.375TyrTrp: 0.375 ± 0.02	0.684TyrTyr: 0.684 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3115 proteins (983925 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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