Propioniferax innocua
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2528 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A542ZSZ2|A0A542ZSZ2_9ACTN BglG family transcriptional antiterminator OS=Propioniferax innocua OX=1753 GN=FB460_1288 PE=4 SV=1
MM1 pKa = 7.52 TEE3 pKa = 4.35 PIKK6 pKa = 10.94 VATVGDD12 pKa = 3.83 IDD14 pKa = 4.6 DD15 pKa = 5.17 DD16 pKa = 4.11 EE17 pKa = 7.37 AIVVPGDD24 pKa = 3.39 VNGTGNPIAVFFTEE38 pKa = 4.1 DD39 pKa = 3.08 EE40 pKa = 4.72 YY41 pKa = 12.02 YY42 pKa = 11.21 ALDD45 pKa = 3.89 DD46 pKa = 4.05 TCTHH50 pKa = 6.89 AEE52 pKa = 3.85 ASLADD57 pKa = 3.85 GWVEE61 pKa = 3.94 GCEE64 pKa = 4.23 VEE66 pKa = 5.15 CPIHH70 pKa = 6.12 AAKK73 pKa = 10.57 FNLCTGQAMNLPATIPVRR91 pKa = 11.84 THH93 pKa = 5.09 KK94 pKa = 11.14 VEE96 pKa = 3.9 VRR98 pKa = 11.84 GDD100 pKa = 4.25 EE101 pKa = 4.02 IWLHH105 pKa = 6.08 PGVPVEE111 pKa = 4.19 GAVLEE116 pKa = 4.38 ADD118 pKa = 3.49 QQ119 pKa = 4.88
Molecular weight: 12.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.795
IPC2_protein 3.999
IPC_protein 3.948
Toseland 3.757
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.821
Rodwell 3.783
Grimsley 3.668
Solomon 3.91
Lehninger 3.859
Nozaki 4.037
DTASelect 4.215
Thurlkill 3.795
EMBOSS 3.834
Sillero 4.062
Patrickios 1.914
IPC_peptide 3.91
IPC2_peptide 4.05
IPC2.peptide.svr19 3.957
Protein with the highest isoelectric point:
>tr|A0A542ZDU1|A0A542ZDU1_9ACTN Propane 2-monooxygenase OS=Propioniferax innocua OX=1753 GN=FB460_2312 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SRR15 pKa = 11.84 THH17 pKa = 6.18 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 SIISSRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.83 GRR40 pKa = 11.84 AKK42 pKa = 10.71 LAGG45 pKa = 3.63
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.705
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.427
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.265
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2528
0
2528
847396
29
2890
335.2
36.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.816 ± 0.056
0.835 ± 0.013
6.391 ± 0.037
6.374 ± 0.05
3.002 ± 0.033
8.994 ± 0.051
2.392 ± 0.025
4.373 ± 0.032
2.275 ± 0.035
9.519 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.376 ± 0.022
2.154 ± 0.022
5.681 ± 0.041
3.149 ± 0.028
7.136 ± 0.054
5.442 ± 0.034
6.098 ± 0.04
8.609 ± 0.041
1.57 ± 0.022
1.813 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here