Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Sordariales; Chaetomiaceae; Podospora; Podospora anserina

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10657 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2B862|B2B862_PODAN Podospora anserina S mat+ genomic DNA chromosome 2 supercontig 2 OS=Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) OX=515849 GN=PODANS_2_13440 PE=3 SV=1
MM1 pKa = 7.58TEE3 pKa = 4.38PEE5 pKa = 4.23NFDD8 pKa = 4.53DD9 pKa = 5.84EE10 pKa = 5.47LFADD14 pKa = 5.63LYY16 pKa = 11.21NDD18 pKa = 4.73DD19 pKa = 5.05DD20 pKa = 4.5AAPAPKK26 pKa = 9.17PAAAAQPVQYY36 pKa = 10.81AAVQPTIEE44 pKa = 4.1TRR46 pKa = 11.84QNDD49 pKa = 4.09SYY51 pKa = 12.04DD52 pKa = 3.4PNQYY56 pKa = 10.73NDD58 pKa = 3.44YY59 pKa = 11.31GGGGEE64 pKa = 4.64NGNINQDD71 pKa = 3.75DD72 pKa = 4.32EE73 pKa = 5.03EE74 pKa = 5.56EE75 pKa = 4.46EE76 pKa = 5.63DD77 pKa = 5.36DD78 pKa = 6.36DD79 pKa = 7.3DD80 pKa = 7.82DD81 pKa = 6.1IDD83 pKa = 4.46FNLGNGPSTTLAPHH97 pKa = 6.82DD98 pKa = 3.71QQDD101 pKa = 4.14YY102 pKa = 11.29NDD104 pKa = 3.5SHH106 pKa = 7.52NNNNNNNSSSSSNNNNSHH124 pKa = 6.16HH125 pKa = 6.5EE126 pKa = 4.07EE127 pKa = 4.05RR128 pKa = 11.84QSYY131 pKa = 8.87SAPSAPPAHH140 pKa = 6.19TKK142 pKa = 10.45GPNAKK147 pKa = 9.88EE148 pKa = 4.11DD149 pKa = 3.59GG150 pKa = 3.85

Molecular weight: 16.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2AXS1|B2AXS1_PODAN Podospora anserina S mat+ genomic DNA chromosome 7 supercontig 1 OS=Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) OX=515849 GN=PODANS_7_11510 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.09WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.73RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.94RR15 pKa = 11.84KK16 pKa = 7.8RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.6MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10657

0

10657

5371942

14

8070

504.1

55.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.436 ± 0.022

1.208 ± 0.008

5.477 ± 0.018

6.379 ± 0.028

3.606 ± 0.016

7.348 ± 0.025

2.385 ± 0.011

4.546 ± 0.017

4.951 ± 0.022

8.76 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.137 ± 0.009

3.598 ± 0.014

6.529 ± 0.03

4.016 ± 0.018

6.183 ± 0.021

7.963 ± 0.028

6.085 ± 0.036

6.281 ± 0.018

1.482 ± 0.008

2.63 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski