Acidithiobacillus sp. GGI-221

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Acidithiobacillia; Acidithiobacillales; Acidithiobacillaceae; Acidithiobacillus; unclassified Acidithiobacillus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4007 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8XMH4|F8XMH4_9PROT Uncharacterized protein OS=Acidithiobacillus sp. GGI-221 OX=872330 GN=GGI1_03401 PE=4 SV=1
MM1 pKa = 7.58AIEE4 pKa = 4.0YY5 pKa = 10.97AEE7 pKa = 4.99DD8 pKa = 3.62GVAAIACDD16 pKa = 4.06DD17 pKa = 4.31DD18 pKa = 6.29ADD20 pKa = 4.36AVGQHH25 pKa = 6.19GARR28 pKa = 11.84DD29 pKa = 3.68LHH31 pKa = 6.82AHH33 pKa = 5.65ATGAADD39 pKa = 4.12DD40 pKa = 4.11QQLLTLEE47 pKa = 5.2LIAA50 pKa = 6.29

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8XLL5|F8XLL5_9PROT Uncharacterized protein OS=Acidithiobacillus sp. GGI-221 OX=872330 GN=GGI1_01523 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVVHH11 pKa = 6.89RR12 pKa = 11.84KK13 pKa = 7.38RR14 pKa = 11.84THH16 pKa = 5.56GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29SGRR28 pKa = 11.84LVLKK32 pKa = 10.26RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.25GRR39 pKa = 11.84QRR41 pKa = 11.84LCPP44 pKa = 3.78

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4007

0

4007

737804

18

1435

184.1

20.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.167 ± 0.056

1.046 ± 0.018

5.13 ± 0.026

5.364 ± 0.049

3.482 ± 0.025

8.272 ± 0.052

2.7 ± 0.026

5.233 ± 0.032

3.194 ± 0.037

10.572 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.024

2.871 ± 0.036

5.292 ± 0.037

4.223 ± 0.034

7.058 ± 0.047

5.473 ± 0.037

5.031 ± 0.034

7.011 ± 0.037

1.616 ± 0.02

2.6 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski