Amino acid dipepetide frequency for Acidithiobacillus sp. GGI-221

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.084AlaAla: 14.084 ± 0.187	1.233AlaCys: 1.233 ± 0.046	5.71AlaAsp: 5.71 ± 0.093	6.7AlaGlu: 6.7 ± 0.11	3.616AlaPhe: 3.616 ± 0.064	9.408AlaGly: 9.408 ± 0.128	2.636AlaHis: 2.636 ± 0.056	5.851AlaIle: 5.851 ± 0.094	3.31AlaLys: 3.31 ± 0.075	12.636AlaLeu: 12.636 ± 0.161	3.279AlaMet: 3.279 ± 0.074	2.822AlaAsn: 2.822 ± 0.08	4.855AlaPro: 4.855 ± 0.088	4.978AlaGln: 4.978 ± 0.116	7.426AlaArg: 7.426 ± 0.112	5.354AlaSer: 5.354 ± 0.087	5.176AlaThr: 5.176 ± 0.082	8.193AlaVal: 8.193 ± 0.111	1.69AlaTrp: 1.69 ± 0.048	2.709AlaTyr: 2.709 ± 0.069	0.003AlaXaa: 0.003 ± 0.002
Cys	1.179CysAla: 1.179 ± 0.04	0.18CysCys: 0.18 ± 0.016	0.478CysAsp: 0.478 ± 0.028	0.461CysGlu: 0.461 ± 0.026	0.352CysPhe: 0.352 ± 0.02	1.012CysGly: 1.012 ± 0.037	0.371CysHis: 0.371 ± 0.025	0.472CysIle: 0.472 ± 0.026	0.317CysLys: 0.317 ± 0.018	0.951CysLeu: 0.951 ± 0.037	0.27CysMet: 0.27 ± 0.019	0.287CysAsn: 0.287 ± 0.018	0.724CysPro: 0.724 ± 0.031	0.369CysGln: 0.369 ± 0.023	0.809CysArg: 0.809 ± 0.04	0.651CysSer: 0.651 ± 0.034	0.506CysThr: 0.506 ± 0.029	0.623CysVal: 0.623 ± 0.036	0.169CysTrp: 0.169 ± 0.014	0.275CysTyr: 0.275 ± 0.021	0.001CysXaa: 0.001 ± 0.001
Asp	5.987AspAla: 5.987 ± 0.112	0.493AspCys: 0.493 ± 0.028	2.6AspAsp: 2.6 ± 0.071	2.781AspGlu: 2.781 ± 0.06	2.116AspPhe: 2.116 ± 0.05	4.368AspGly: 4.368 ± 0.078	1.378AspHis: 1.378 ± 0.037	2.793AspIle: 2.793 ± 0.061	1.552AspLys: 1.552 ± 0.051	5.562AspLeu: 5.562 ± 0.085	1.343AspMet: 1.343 ± 0.042	1.3AspAsn: 1.3 ± 0.047	3.048AspPro: 3.048 ± 0.069	1.832AspGln: 1.832 ± 0.051	3.426AspArg: 3.426 ± 0.078	2.261AspSer: 2.261 ± 0.052	2.498AspThr: 2.498 ± 0.058	3.33AspVal: 3.33 ± 0.078	1.065AspTrp: 1.065 ± 0.044	1.567AspTyr: 1.567 ± 0.046	0.001AspXaa: 0.001 ± 0.001
Glu	6.117GluAla: 6.117 ± 0.109	0.454GluCys: 0.454 ± 0.029	2.802GluAsp: 2.802 ± 0.067	3.108GluGlu: 3.108 ± 0.079	1.521GluPhe: 1.521 ± 0.046	3.885GluGly: 3.885 ± 0.077	1.45GluHis: 1.45 ± 0.039	3.369GluIle: 3.369 ± 0.066	2.356GluLys: 2.356 ± 0.067	4.793GluLeu: 4.793 ± 0.087	1.584GluMet: 1.584 ± 0.052	1.88GluAsn: 1.88 ± 0.052	2.049GluPro: 2.049 ± 0.057	2.517GluGln: 2.517 ± 0.065	4.367GluArg: 4.367 ± 0.094	2.757GluSer: 2.757 ± 0.06	2.884GluThr: 2.884 ± 0.067	3.742GluVal: 3.742 ± 0.064	0.77GluTrp: 0.77 ± 0.029	1.237GluTyr: 1.237 ± 0.04	0.001GluXaa: 0.001 ± 0.001
Phe	3.65PheAla: 3.65 ± 0.067	0.507PheCys: 0.507 ± 0.026	1.856PheAsp: 1.856 ± 0.054	1.415PheGlu: 1.415 ± 0.044	1.456PhePhe: 1.456 ± 0.045	3.071PheGly: 3.071 ± 0.073	1.018PheHis: 1.018 ± 0.036	1.789PheIle: 1.789 ± 0.055	0.87PheLys: 0.87 ± 0.034	3.814PheLeu: 3.814 ± 0.073	0.872PheMet: 0.872 ± 0.034	1.018PheAsn: 1.018 ± 0.038	1.809PhePro: 1.809 ± 0.045	1.358PheGln: 1.358 ± 0.045	2.17PheArg: 2.17 ± 0.056	2.28PheSer: 2.28 ± 0.062	1.813PheThr: 1.813 ± 0.052	2.322PheVal: 2.322 ± 0.054	0.649PheTrp: 0.649 ± 0.032	1.083PheTyr: 1.083 ± 0.042	0.001PheXaa: 0.001 ± 0.001
Gly	8.02GlyAla: 8.02 ± 0.142	0.973GlyCys: 0.973 ± 0.038	4.078GlyAsp: 4.078 ± 0.075	4.377GlyGlu: 4.377 ± 0.086	3.153GlyPhe: 3.153 ± 0.072	6.823GlyGly: 6.823 ± 0.11	2.329GlyHis: 2.329 ± 0.059	4.923GlyIle: 4.923 ± 0.09	3.524GlyLys: 3.524 ± 0.074	8.258GlyLeu: 8.258 ± 0.123	2.621GlyMet: 2.621 ± 0.055	2.609GlyAsn: 2.609 ± 0.063	3.2GlyPro: 3.2 ± 0.073	3.429GlyGln: 3.429 ± 0.068	5.42GlyArg: 5.42 ± 0.091	4.442GlySer: 4.442 ± 0.087	4.425GlyThr: 4.425 ± 0.102	6.323GlyVal: 6.323 ± 0.105	1.426GlyTrp: 1.426 ± 0.042	2.365GlyTyr: 2.365 ± 0.054	0.004GlyXaa: 0.004 ± 0.002
His	2.651HisAla: 2.651 ± 0.066	0.4HisCys: 0.4 ± 0.024	1.258HisAsp: 1.258 ± 0.039	1.179HisGlu: 1.179 ± 0.041	1.132HisPhe: 1.132 ± 0.036	2.522HisGly: 2.522 ± 0.063	0.966HisHis: 0.966 ± 0.042	1.346HisIle: 1.346 ± 0.042	0.717HisLys: 0.717 ± 0.03	3.071HisLeu: 3.071 ± 0.072	0.648HisMet: 0.648 ± 0.03	0.644HisAsn: 0.644 ± 0.032	1.94HisPro: 1.94 ± 0.064	1.048HisGln: 1.048 ± 0.036	1.872HisArg: 1.872 ± 0.056	1.32HisSer: 1.32 ± 0.048	1.187HisThr: 1.187 ± 0.043	1.533HisVal: 1.533 ± 0.052	0.583HisTrp: 0.583 ± 0.031	0.98HisTyr: 0.98 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	6.068IleAla: 6.068 ± 0.105	0.577IleCys: 0.577 ± 0.027	2.632IleAsp: 2.632 ± 0.057	2.662IleGlu: 2.662 ± 0.069	1.895IlePhe: 1.895 ± 0.048	4.303IleGly: 4.303 ± 0.089	1.586IleHis: 1.586 ± 0.046	2.513IleIle: 2.513 ± 0.061	1.504IleLys: 1.504 ± 0.044	5.594IleLeu: 5.594 ± 0.084	1.091IleMet: 1.091 ± 0.038	1.655IleAsn: 1.655 ± 0.048	3.151IlePro: 3.151 ± 0.064	1.98IleGln: 1.98 ± 0.057	3.513IleArg: 3.513 ± 0.071	3.338IleSer: 3.338 ± 0.067	2.81IleThr: 2.81 ± 0.065	3.393IleVal: 3.393 ± 0.072	0.75IleTrp: 0.75 ± 0.035	1.312IleTyr: 1.312 ± 0.04	0.003IleXaa: 0.003 ± 0.002
Lys	3.769LysAla: 3.769 ± 0.082	0.182LysCys: 0.182 ± 0.018	1.751LysAsp: 1.751 ± 0.052	1.838LysGlu: 1.838 ± 0.053	0.744LysPhe: 0.744 ± 0.034	2.597LysGly: 2.597 ± 0.06	0.693LysHis: 0.693 ± 0.028	1.72LysIle: 1.72 ± 0.048	1.461LysLys: 1.461 ± 0.059	2.758LysLeu: 2.758 ± 0.064	0.873LysMet: 0.873 ± 0.03	1.163LysAsn: 1.163 ± 0.043	1.8LysPro: 1.8 ± 0.052	1.179LysGln: 1.179 ± 0.04	2.211LysArg: 2.211 ± 0.058	1.885LysSer: 1.885 ± 0.056	2.051LysThr: 2.051 ± 0.053	2.131LysVal: 2.131 ± 0.047	0.396LysTrp: 0.396 ± 0.024	0.736LysTyr: 0.736 ± 0.033	0.003LysXaa: 0.003 ± 0.002
Leu	12.342LeuAla: 12.342 ± 0.14	0.995LeuCys: 0.995 ± 0.038	5.644LeuAsp: 5.644 ± 0.085	5.657LeuGlu: 5.657 ± 0.103	3.585LeuPhe: 3.585 ± 0.078	8.17LeuGly: 8.17 ± 0.123	2.892LeuHis: 2.892 ± 0.071	4.614LeuIle: 4.614 ± 0.089	3.025LeuLys: 3.025 ± 0.077	11.603LeuLeu: 11.603 ± 0.178	2.394LeuMet: 2.394 ± 0.057	2.68LeuAsn: 2.68 ± 0.056	6.382LeuPro: 6.382 ± 0.092	4.856LeuGln: 4.856 ± 0.091	8.424LeuArg: 8.424 ± 0.129	6.487LeuSer: 6.487 ± 0.101	5.108LeuThr: 5.108 ± 0.072	6.794LeuVal: 6.794 ± 0.105	1.575LeuTrp: 1.575 ± 0.052	2.491LeuTyr: 2.491 ± 0.062	0.001LeuXaa: 0.001 ± 0.001
Met	3.368MetAla: 3.368 ± 0.081	0.159MetCys: 0.159 ± 0.015	1.57MetAsp: 1.57 ± 0.04	1.518MetGlu: 1.518 ± 0.048	0.622MetPhe: 0.622 ± 0.029	2.209MetGly: 2.209 ± 0.053	0.567MetHis: 0.567 ± 0.029	1.286MetIle: 1.286 ± 0.042	0.838MetLys: 0.838 ± 0.035	2.61MetLeu: 2.61 ± 0.068	0.703MetMet: 0.703 ± 0.035	0.914MetAsn: 0.914 ± 0.035	1.571MetPro: 1.571 ± 0.047	1.082MetGln: 1.082 ± 0.04	1.77MetArg: 1.77 ± 0.045	1.552MetSer: 1.552 ± 0.049	1.677MetThr: 1.677 ± 0.053	2.002MetVal: 2.002 ± 0.05	0.243MetTrp: 0.243 ± 0.017	0.373MetTyr: 0.373 ± 0.024	0.001MetXaa: 0.001 ± 0.002
Asn	3.204AsnAla: 3.204 ± 0.068	0.27AsnCys: 0.27 ± 0.02	1.495AsnAsp: 1.495 ± 0.045	1.106AsnGlu: 1.106 ± 0.04	0.953AsnPhe: 0.953 ± 0.04	2.61AsnGly: 2.61 ± 0.076	0.705AsnHis: 0.705 ± 0.032	1.618AsnIle: 1.618 ± 0.058	0.816AsnLys: 0.816 ± 0.037	2.943AsnLeu: 2.943 ± 0.062	0.68AsnMet: 0.68 ± 0.03	1.004AsnAsn: 1.004 ± 0.05	2.201AsnPro: 2.201 ± 0.064	1.133AsnGln: 1.133 ± 0.048	1.902AsnArg: 1.902 ± 0.058	1.475AsnSer: 1.475 ± 0.056	1.659AsnThr: 1.659 ± 0.054	1.704AsnVal: 1.704 ± 0.052	0.428AsnTrp: 0.428 ± 0.029	0.798AsnTyr: 0.798 ± 0.041	0.001AsnXaa: 0.001 ± 0.001
Pro	5.777ProAla: 5.777 ± 0.092	0.587ProCys: 0.587 ± 0.03	3.237ProAsp: 3.237 ± 0.078	3.75ProGlu: 3.75 ± 0.078	1.923ProPhe: 1.923 ± 0.048	4.614ProGly: 4.614 ± 0.085	1.334ProHis: 1.334 ± 0.044	2.384ProIle: 2.384 ± 0.063	1.613ProLys: 1.613 ± 0.049	5.287ProLeu: 5.287 ± 0.102	1.423ProMet: 1.423 ± 0.046	1.29ProAsn: 1.29 ± 0.042	2.579ProPro: 2.579 ± 0.075	2.265ProGln: 2.265 ± 0.063	2.928ProArg: 2.928 ± 0.066	2.704ProSer: 2.704 ± 0.063	2.315ProThr: 2.315 ± 0.053	4.451ProVal: 4.451 ± 0.08	0.976ProTrp: 0.976 ± 0.035	1.476ProTyr: 1.476 ± 0.05	0.004ProXaa: 0.004 ± 0.002
Gln	5.022GlnAla: 5.022 ± 0.101	0.384GlnCys: 0.384 ± 0.024	1.846GlnAsp: 1.846 ± 0.05	2.451GlnGlu: 2.451 ± 0.071	1.243GlnPhe: 1.243 ± 0.041	3.365GlnGly: 3.365 ± 0.076	1.156GlnHis: 1.156 ± 0.041	2.292GlnIle: 2.292 ± 0.063	1.586GlnLys: 1.586 ± 0.046	3.453GlnLeu: 3.453 ± 0.077	1.137GlnMet: 1.137 ± 0.038	1.259GlnAsn: 1.259 ± 0.049	2.015GlnPro: 2.015 ± 0.057	2.155GlnGln: 2.155 ± 0.063	3.479GlnArg: 3.479 ± 0.072	2.479GlnSer: 2.479 ± 0.063	2.151GlnThr: 2.151 ± 0.069	3.01GlnVal: 3.01 ± 0.075	0.698GlnTrp: 0.698 ± 0.03	1.042GlnTyr: 1.042 ± 0.044	0.001GlnXaa: 0.001 ± 0.001
Arg	6.564ArgAla: 6.564 ± 0.082	0.751ArgCys: 0.751 ± 0.036	3.588ArgAsp: 3.588 ± 0.072	4.234ArgGlu: 4.234 ± 0.09	2.819ArgPhe: 2.819 ± 0.067	4.604ArgGly: 4.604 ± 0.085	2.216ArgHis: 2.216 ± 0.056	3.967ArgIle: 3.967 ± 0.078	2.663ArgLys: 2.663 ± 0.062	7.844ArgLeu: 7.844 ± 0.101	2.071ArgMet: 2.071 ± 0.052	2.037ArgAsn: 2.037 ± 0.044	3.387ArgPro: 3.387 ± 0.071	3.426ArgGln: 3.426 ± 0.068	5.836ArgArg: 5.836 ± 0.112	3.669ArgSer: 3.669 ± 0.078	2.727ArgThr: 2.727 ± 0.066	4.629ArgVal: 4.629 ± 0.075	1.339ArgTrp: 1.339 ± 0.05	2.205ArgTyr: 2.205 ± 0.049	0.001ArgXaa: 0.001 ± 0.001
Ser	6.137SerAla: 6.137 ± 0.107	0.632SerCys: 0.632 ± 0.03	2.48SerAsp: 2.48 ± 0.061	2.422SerGlu: 2.422 ± 0.057	1.984SerPhe: 1.984 ± 0.052	5.657SerGly: 5.657 ± 0.097	1.378SerHis: 1.378 ± 0.041	2.849SerIle: 2.849 ± 0.065	1.514SerLys: 1.514 ± 0.039	5.718SerLeu: 5.718 ± 0.09	1.555SerMet: 1.555 ± 0.048	1.537SerAsn: 1.537 ± 0.054	3.162SerPro: 3.162 ± 0.066	1.956SerGln: 1.956 ± 0.056	3.647SerArg: 3.647 ± 0.066	3.166SerSer: 3.166 ± 0.083	2.892SerThr: 2.892 ± 0.059	3.783SerVal: 3.783 ± 0.073	0.835SerTrp: 0.835 ± 0.033	1.422SerTyr: 1.422 ± 0.054	0.001SerXaa: 0.001 ± 0.001
Thr	5.725ThrAla: 5.725 ± 0.088	0.511ThrCys: 0.511 ± 0.029	2.483ThrAsp: 2.483 ± 0.059	2.33ThrGlu: 2.33 ± 0.059	1.624ThrPhe: 1.624 ± 0.042	4.829ThrGly: 4.829 ± 0.102	1.237ThrHis: 1.237 ± 0.037	2.764ThrIle: 2.764 ± 0.071	1.246ThrLys: 1.246 ± 0.045	6.0ThrLeu: 6.0 ± 0.097	1.262ThrMet: 1.262 ± 0.041	1.246ThrAsn: 1.246 ± 0.049	3.218ThrPro: 3.218 ± 0.07	1.827ThrGln: 1.827 ± 0.056	3.203ThrArg: 3.203 ± 0.069	2.452ThrSer: 2.452 ± 0.06	2.551ThrThr: 2.551 ± 0.061	3.872ThrVal: 3.872 ± 0.08	0.755ThrTrp: 0.755 ± 0.03	1.179ThrTyr: 1.179 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	7.853ValAla: 7.853 ± 0.112	0.674ValCys: 0.674 ± 0.031	3.817ValAsp: 3.817 ± 0.073	3.776ValGlu: 3.776 ± 0.078	2.529ValPhe: 2.529 ± 0.054	5.213ValGly: 5.213 ± 0.092	1.846ValHis: 1.846 ± 0.05	3.851ValIle: 3.851 ± 0.069	1.895ValLys: 1.895 ± 0.06	7.841ValLeu: 7.841 ± 0.113	1.824ValMet: 1.824 ± 0.058	2.09ValAsn: 2.09 ± 0.057	3.607ValPro: 3.607 ± 0.065	2.69ValGln: 2.69 ± 0.061	4.638ValArg: 4.638 ± 0.081	4.175ValSer: 4.175 ± 0.076	3.826ValThr: 3.826 ± 0.072	5.385ValVal: 5.385 ± 0.101	1.021ValTrp: 1.021 ± 0.041	1.56ValTyr: 1.56 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	1.323TrpAla: 1.323 ± 0.048	0.188TrpCys: 0.188 ± 0.017	0.702TrpAsp: 0.702 ± 0.031	0.787TrpGlu: 0.787 ± 0.034	0.549TrpPhe: 0.549 ± 0.029	1.056TrpGly: 1.056 ± 0.042	0.47TrpHis: 0.47 ± 0.029	0.893TrpIle: 0.893 ± 0.037	0.576TrpLys: 0.576 ± 0.031	1.979TrpLeu: 1.979 ± 0.056	0.496TrpMet: 0.496 ± 0.023	0.48TrpAsn: 0.48 ± 0.026	0.794TrpPro: 0.794 ± 0.037	0.88TrpGln: 0.88 ± 0.035	1.397TrpArg: 1.397 ± 0.046	0.869TrpSer: 0.869 ± 0.037	0.815TrpThr: 0.815 ± 0.031	1.168TrpVal: 1.168 ± 0.046	0.347TrpTrp: 0.347 ± 0.027	0.388TrpTyr: 0.388 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.831TyrAla: 2.831 ± 0.073	0.31TyrCys: 0.31 ± 0.022	1.278TyrAsp: 1.278 ± 0.043	1.091TyrGlu: 1.091 ± 0.04	1.003TyrPhe: 1.003 ± 0.036	2.4TyrGly: 2.4 ± 0.051	0.766TyrHis: 0.766 ± 0.032	1.034TyrIle: 1.034 ± 0.039	0.553TyrLys: 0.553 ± 0.03	2.998TyrLeu: 2.998 ± 0.058	0.506TyrMet: 0.506 ± 0.027	0.792TyrAsn: 0.792 ± 0.046	1.42TyrPro: 1.42 ± 0.044	1.265TyrGln: 1.265 ± 0.039	2.139TyrArg: 2.139 ± 0.055	1.412TyrSer: 1.412 ± 0.044	1.242TyrThr: 1.242 ± 0.046	1.716TyrVal: 1.716 ± 0.048	0.443TyrTrp: 0.443 ± 0.026	0.8TyrTyr: 0.8 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.003XaaPhe: 0.003 ± 0.002	0.003XaaGly: 0.003 ± 0.002	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.004XaaLys: 0.004 ± 0.003	0.005XaaLeu: 0.005 ± 0.003	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.003XaaSer: 0.003 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.005XaaVal: 0.005 ± 0.003	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4007 proteins (737804 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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