Xenorhabdus cabanillasii JM26
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4375 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W1IMQ8|W1IMQ8_9GAMM Major outer membrane lipoprotein Lpp OS=Xenorhabdus cabanillasii JM26 OX=1427517 GN=lpp PE=3 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.27 NINYY6 pKa = 9.61 SPLSIAQHH14 pKa = 5.72 NDD16 pKa = 2.74 GYY18 pKa = 10.54 IIKK21 pKa = 9.85 QVSDD25 pKa = 3.0 SWSVEE30 pKa = 3.3 FDD32 pKa = 3.4 FDD34 pKa = 4.31 IMILQRR40 pKa = 11.84 CDD42 pKa = 3.22 VYY44 pKa = 10.88 IDD46 pKa = 4.36 SGDD49 pKa = 3.74 DD50 pKa = 3.27 PALL53 pKa = 4.01
Molecular weight: 6.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.923
IPC_protein 3.846
Toseland 3.617
ProMoST 4.062
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.897
Rodwell 3.681
Grimsley 3.541
Solomon 3.846
Lehninger 3.808
Nozaki 4.012
DTASelect 4.317
Thurlkill 3.719
EMBOSS 3.884
Sillero 3.973
Patrickios 1.952
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.878
Protein with the highest isoelectric point:
>tr|W1JAX3|W1JAX3_9GAMM Uncharacterized lipoprotein YifL OS=Xenorhabdus cabanillasii JM26 OX=1427517 GN=XCR1_4300009 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.68 RR12 pKa = 11.84 NRR14 pKa = 11.84 THH16 pKa = 7.28 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSKK46 pKa = 10.99
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4375
0
4375
1251180
20
4657
286.0
31.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.081 ± 0.045
1.109 ± 0.015
5.187 ± 0.032
6.046 ± 0.034
3.955 ± 0.025
6.831 ± 0.04
2.343 ± 0.021
6.895 ± 0.041
5.257 ± 0.039
10.436 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.551 ± 0.018
4.529 ± 0.035
4.196 ± 0.03
4.671 ± 0.04
5.223 ± 0.033
6.416 ± 0.029
5.366 ± 0.03
6.354 ± 0.028
1.336 ± 0.015
3.216 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here