Amino acid dipepetide frequency for Fusibacter sp. 3D3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.208AlaAla: 4.208 ± 0.075	0.783AlaCys: 0.783 ± 0.024	3.259AlaAsp: 3.259 ± 0.048	4.105AlaGlu: 4.105 ± 0.056	3.535AlaPhe: 3.535 ± 0.057	4.189AlaGly: 4.189 ± 0.074	1.435AlaHis: 1.435 ± 0.029	6.304AlaIle: 6.304 ± 0.067	4.26AlaLys: 4.26 ± 0.064	8.059AlaLeu: 8.059 ± 0.084	2.081AlaMet: 2.081 ± 0.035	2.7AlaAsn: 2.7 ± 0.044	1.825AlaPro: 1.825 ± 0.044	2.178AlaGln: 2.178 ± 0.039	2.046AlaArg: 2.046 ± 0.036	4.301AlaSer: 4.301 ± 0.064	3.466AlaThr: 3.466 ± 0.059	4.853AlaVal: 4.853 ± 0.066	0.432AlaTrp: 0.432 ± 0.017	3.015AlaTyr: 3.015 ± 0.045	0.003AlaXaa: 0.003 ± 0.002
Cys	0.578CysAla: 0.578 ± 0.019	0.131CysCys: 0.131 ± 0.01	0.687CysAsp: 0.687 ± 0.022	0.721CysGlu: 0.721 ± 0.022	0.482CysPhe: 0.482 ± 0.019	0.895CysGly: 0.895 ± 0.029	0.266CysHis: 0.266 ± 0.014	0.826CysIle: 0.826 ± 0.024	0.621CysLys: 0.621 ± 0.018	0.814CysLeu: 0.814 ± 0.026	0.255CysMet: 0.255 ± 0.014	0.424CysAsn: 0.424 ± 0.019	0.436CysPro: 0.436 ± 0.022	0.276CysGln: 0.276 ± 0.015	0.301CysArg: 0.301 ± 0.013	0.619CysSer: 0.619 ± 0.021	0.471CysThr: 0.471 ± 0.019	0.612CysVal: 0.612 ± 0.022	0.053CysTrp: 0.053 ± 0.006	0.37CysTyr: 0.37 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.796AspAla: 3.796 ± 0.056	0.489AspCys: 0.489 ± 0.021	2.804AspAsp: 2.804 ± 0.055	4.431AspGlu: 4.431 ± 0.059	2.786AspPhe: 2.786 ± 0.046	3.072AspGly: 3.072 ± 0.062	1.301AspHis: 1.301 ± 0.032	4.687AspIle: 4.687 ± 0.061	3.382AspLys: 3.382 ± 0.048	5.538AspLeu: 5.538 ± 0.068	1.497AspMet: 1.497 ± 0.032	2.014AspAsn: 2.014 ± 0.04	1.741AspPro: 1.741 ± 0.036	2.367AspGln: 2.367 ± 0.045	2.078AspArg: 2.078 ± 0.043	2.923AspSer: 2.923 ± 0.056	2.514AspThr: 2.514 ± 0.046	3.812AspVal: 3.812 ± 0.05	0.459AspTrp: 0.459 ± 0.018	2.72AspTyr: 2.72 ± 0.043	0.003AspXaa: 0.003 ± 0.002
Glu	5.61GluAla: 5.61 ± 0.075	0.482GluCys: 0.482 ± 0.019	3.656GluAsp: 3.656 ± 0.049	4.473GluGlu: 4.473 ± 0.068	2.561GluPhe: 2.561 ± 0.042	4.334GluGly: 4.334 ± 0.066	1.405GluHis: 1.405 ± 0.031	7.011GluIle: 7.011 ± 0.075	5.995GluLys: 5.995 ± 0.069	6.391GluLeu: 6.391 ± 0.077	2.403GluMet: 2.403 ± 0.049	4.103GluAsn: 4.103 ± 0.053	1.786GluPro: 1.786 ± 0.036	2.36GluGln: 2.36 ± 0.042	2.938GluArg: 2.938 ± 0.054	4.208GluSer: 4.208 ± 0.058	3.988GluThr: 3.988 ± 0.052	4.653GluVal: 4.653 ± 0.065	0.553GluTrp: 0.553 ± 0.021	2.295GluTyr: 2.295 ± 0.044	0.005GluXaa: 0.005 ± 0.002
Phe	2.65PheAla: 2.65 ± 0.045	0.457PheCys: 0.457 ± 0.02	2.918PheAsp: 2.918 ± 0.044	3.683PheGlu: 3.683 ± 0.061	2.023PhePhe: 2.023 ± 0.042	3.006PheGly: 3.006 ± 0.056	0.661PheHis: 0.661 ± 0.024	3.926PheIle: 3.926 ± 0.07	3.412PheLys: 3.412 ± 0.051	4.008PheLeu: 4.008 ± 0.07	1.333PheMet: 1.333 ± 0.031	2.414PheAsn: 2.414 ± 0.039	1.3PhePro: 1.3 ± 0.029	1.094PheGln: 1.094 ± 0.026	1.465PheArg: 1.465 ± 0.034	3.224PheSer: 3.224 ± 0.045	2.412PheThr: 2.412 ± 0.048	2.913PheVal: 2.913 ± 0.044	0.34PheTrp: 0.34 ± 0.017	1.873PheTyr: 1.873 ± 0.039	0.001PheXaa: 0.001 ± 0.001
Gly	4.482GlyAla: 4.482 ± 0.065	0.791GlyCys: 0.791 ± 0.022	3.317GlyAsp: 3.317 ± 0.057	3.878GlyGlu: 3.878 ± 0.054	3.235GlyPhe: 3.235 ± 0.047	4.17GlyGly: 4.17 ± 0.077	1.402GlyHis: 1.402 ± 0.029	6.335GlyIle: 6.335 ± 0.073	4.6GlyLys: 4.6 ± 0.062	6.102GlyLeu: 6.102 ± 0.075	1.906GlyMet: 1.906 ± 0.044	2.855GlyAsn: 2.855 ± 0.075	1.324GlyPro: 1.324 ± 0.034	1.821GlyGln: 1.821 ± 0.04	2.24GlyArg: 2.24 ± 0.038	3.685GlySer: 3.685 ± 0.064	3.783GlyThr: 3.783 ± 0.065	4.6GlyVal: 4.6 ± 0.066	0.543GlyTrp: 0.543 ± 0.021	2.907GlyTyr: 2.907 ± 0.048	0.005GlyXaa: 0.005 ± 0.002
His	1.169HisAla: 1.169 ± 0.03	0.233HisCys: 0.233 ± 0.014	0.987HisAsp: 0.987 ± 0.028	1.37HisGlu: 1.37 ± 0.031	1.137HisPhe: 1.137 ± 0.028	1.112HisGly: 1.112 ± 0.03	0.543HisHis: 0.543 ± 0.02	1.642HisIle: 1.642 ± 0.037	1.312HisLys: 1.312 ± 0.032	1.994HisLeu: 1.994 ± 0.038	0.568HisMet: 0.568 ± 0.02	0.824HisAsn: 0.824 ± 0.027	0.856HisPro: 0.856 ± 0.023	0.707HisGln: 0.707 ± 0.023	0.715HisArg: 0.715 ± 0.022	1.091HisSer: 1.091 ± 0.027	0.898HisThr: 0.898 ± 0.025	1.234HisVal: 1.234 ± 0.03	0.185HisTrp: 0.185 ± 0.01	1.006HisTyr: 1.006 ± 0.031	0.003HisXaa: 0.003 ± 0.002
Ile	6.078IleAla: 6.078 ± 0.071	0.96IleCys: 0.96 ± 0.029	5.171IleAsp: 5.171 ± 0.053	7.293IleGlu: 7.293 ± 0.071	3.534IlePhe: 3.534 ± 0.055	5.855IleGly: 5.855 ± 0.078	1.666IleHis: 1.666 ± 0.041	6.972IleIle: 6.972 ± 0.088	6.519IleLys: 6.519 ± 0.075	8.29IleLeu: 8.29 ± 0.082	2.323IleMet: 2.323 ± 0.039	4.322IleAsn: 4.322 ± 0.065	3.114IlePro: 3.114 ± 0.044	2.921IleGln: 2.921 ± 0.045	3.042IleArg: 3.042 ± 0.053	6.175IleSer: 6.175 ± 0.072	4.653IleThr: 4.653 ± 0.07	5.676IleVal: 5.676 ± 0.068	0.568IleTrp: 0.568 ± 0.019	2.956IleTyr: 2.956 ± 0.051	0.003IleXaa: 0.003 ± 0.002
Lys	5.475LysAla: 5.475 ± 0.063	0.659LysCys: 0.659 ± 0.023	3.694LysAsp: 3.694 ± 0.056	5.407LysGlu: 5.407 ± 0.074	2.427LysPhe: 2.427 ± 0.048	4.447LysGly: 4.447 ± 0.059	1.38LysHis: 1.38 ± 0.03	6.201LysIle: 6.201 ± 0.076	5.641LysLys: 5.641 ± 0.07	6.386LysLeu: 6.386 ± 0.068	2.527LysMet: 2.527 ± 0.043	3.89LysAsn: 3.89 ± 0.06	2.207LysPro: 2.207 ± 0.037	2.434LysGln: 2.434 ± 0.039	3.077LysArg: 3.077 ± 0.048	4.68LysSer: 4.68 ± 0.056	4.499LysThr: 4.499 ± 0.058	4.761LysVal: 4.761 ± 0.059	0.707LysTrp: 0.707 ± 0.021	2.861LysTyr: 2.861 ± 0.045	0.005LysXaa: 0.005 ± 0.002
Leu	5.769LeuAla: 5.769 ± 0.059	0.938LeuCys: 0.938 ± 0.025	5.257LeuAsp: 5.257 ± 0.062	7.525LeuGlu: 7.525 ± 0.09	4.322LeuPhe: 4.322 ± 0.071	6.188LeuGly: 6.188 ± 0.064	1.426LeuHis: 1.426 ± 0.03	8.132LeuIle: 8.132 ± 0.097	8.353LeuLys: 8.353 ± 0.079	9.172LeuLeu: 9.172 ± 0.115	3.147LeuMet: 3.147 ± 0.053	5.31LeuAsn: 5.31 ± 0.071	3.085LeuPro: 3.085 ± 0.044	2.418LeuGln: 2.418 ± 0.04	3.176LeuArg: 3.176 ± 0.055	7.338LeuSer: 7.338 ± 0.081	5.276LeuThr: 5.276 ± 0.062	5.719LeuVal: 5.719 ± 0.064	0.662LeuTrp: 0.662 ± 0.024	3.597LeuTyr: 3.597 ± 0.056	0.004LeuXaa: 0.004 ± 0.002
Met	2.238MetAla: 2.238 ± 0.043	0.226MetCys: 0.226 ± 0.012	1.719MetAsp: 1.719 ± 0.037	1.604MetGlu: 1.604 ± 0.034	0.99MetPhe: 0.99 ± 0.029	2.026MetGly: 2.026 ± 0.036	0.523MetHis: 0.523 ± 0.018	2.825MetIle: 2.825 ± 0.047	2.45MetLys: 2.45 ± 0.04	2.723MetLeu: 2.723 ± 0.046	0.981MetMet: 0.981 ± 0.026	1.677MetAsn: 1.677 ± 0.039	1.241MetPro: 1.241 ± 0.028	0.819MetGln: 0.819 ± 0.025	1.161MetArg: 1.161 ± 0.031	1.985MetSer: 1.985 ± 0.036	1.85MetThr: 1.85 ± 0.042	1.792MetVal: 1.792 ± 0.034	0.169MetTrp: 0.169 ± 0.011	0.784MetTyr: 0.784 ± 0.022	0.001MetXaa: 0.001 ± 0.001
Asn	3.636AsnAla: 3.636 ± 0.051	0.522AsnCys: 0.522 ± 0.019	2.458AsnAsp: 2.458 ± 0.053	3.453AsnGlu: 3.453 ± 0.051	2.0AsnPhe: 2.0 ± 0.039	3.176AsnGly: 3.176 ± 0.058	1.103AsnHis: 1.103 ± 0.029	4.208AsnIle: 4.208 ± 0.064	3.163AsnLys: 3.163 ± 0.051	4.642AsnLeu: 4.642 ± 0.064	1.352AsnMet: 1.352 ± 0.029	2.222AsnAsn: 2.222 ± 0.051	2.059AsnPro: 2.059 ± 0.047	2.112AsnGln: 2.112 ± 0.045	2.057AsnArg: 2.057 ± 0.038	2.751AsnSer: 2.751 ± 0.051	2.639AsnThr: 2.639 ± 0.06	3.075AsnVal: 3.075 ± 0.057	0.404AsnTrp: 0.404 ± 0.018	2.026AsnTyr: 2.026 ± 0.04	0.005AsnXaa: 0.005 ± 0.002
Pro	1.801ProAla: 1.801 ± 0.042	0.3ProCys: 0.3 ± 0.013	1.719ProAsp: 1.719 ± 0.04	2.608ProGlu: 2.608 ± 0.052	1.601ProPhe: 1.601 ± 0.035	1.501ProGly: 1.501 ± 0.034	0.634ProHis: 0.634 ± 0.021	2.784ProIle: 2.784 ± 0.046	2.117ProLys: 2.117 ± 0.04	3.071ProLeu: 3.071 ± 0.05	0.928ProMet: 0.928 ± 0.026	1.623ProAsn: 1.623 ± 0.033	0.789ProPro: 0.789 ± 0.034	0.948ProGln: 0.948 ± 0.025	0.86ProArg: 0.86 ± 0.027	2.151ProSer: 2.151 ± 0.04	1.696ProThr: 1.696 ± 0.04	2.384ProVal: 2.384 ± 0.039	0.268ProTrp: 0.268 ± 0.014	1.459ProTyr: 1.459 ± 0.03	0.003ProXaa: 0.003 ± 0.002
Gln	1.951GlnAla: 1.951 ± 0.042	0.262GlnCys: 0.262 ± 0.013	1.392GlnAsp: 1.392 ± 0.032	1.945GlnGlu: 1.945 ± 0.037	1.524GlnPhe: 1.524 ± 0.034	1.752GlnGly: 1.752 ± 0.033	0.502GlnHis: 0.502 ± 0.019	3.071GlnIle: 3.071 ± 0.042	2.657GlnLys: 2.657 ± 0.045	3.064GlnLeu: 3.064 ± 0.048	1.193GlnMet: 1.193 ± 0.027	1.921GlnAsn: 1.921 ± 0.039	0.748GlnPro: 0.748 ± 0.021	0.854GlnGln: 0.854 ± 0.022	1.206GlnArg: 1.206 ± 0.03	2.075GlnSer: 2.075 ± 0.039	1.721GlnThr: 1.721 ± 0.033	2.071GlnVal: 2.071 ± 0.033	0.293GlnTrp: 0.293 ± 0.014	1.501GlnTyr: 1.501 ± 0.037	0.003GlnXaa: 0.003 ± 0.002
Arg	2.378ArgAla: 2.378 ± 0.039	0.355ArgCys: 0.355 ± 0.018	1.824ArgAsp: 1.824 ± 0.041	2.568ArgGlu: 2.568 ± 0.047	1.717ArgPhe: 1.717 ± 0.04	2.063ArgGly: 2.063 ± 0.038	0.744ArgHis: 0.744 ± 0.024	3.378ArgIle: 3.378 ± 0.05	2.653ArgLys: 2.653 ± 0.047	3.554ArgLeu: 3.554 ± 0.052	1.085ArgMet: 1.085 ± 0.029	1.751ArgAsn: 1.751 ± 0.038	1.006ArgPro: 1.006 ± 0.024	1.189ArgGln: 1.189 ± 0.029	1.437ArgArg: 1.437 ± 0.033	2.026ArgSer: 2.026 ± 0.038	1.866ArgThr: 1.866 ± 0.032	2.481ArgVal: 2.481 ± 0.042	0.282ArgTrp: 0.282 ± 0.014	1.514ArgTyr: 1.514 ± 0.034	0.002ArgXaa: 0.002 ± 0.001
Ser	4.021SerAla: 4.021 ± 0.059	0.543SerCys: 0.543 ± 0.023	3.737SerAsp: 3.737 ± 0.059	4.743SerGlu: 4.743 ± 0.056	2.963SerPhe: 2.963 ± 0.044	4.748SerGly: 4.748 ± 0.078	1.198SerHis: 1.198 ± 0.03	5.67SerIle: 5.67 ± 0.068	4.973SerLys: 4.973 ± 0.067	5.737SerLeu: 5.737 ± 0.066	1.766SerMet: 1.766 ± 0.036	3.091SerAsn: 3.091 ± 0.057	1.836SerPro: 1.836 ± 0.036	1.988SerGln: 1.988 ± 0.037	2.303SerArg: 2.303 ± 0.042	3.977SerSer: 3.977 ± 0.074	3.346SerThr: 3.346 ± 0.063	4.424SerVal: 4.424 ± 0.059	0.45SerTrp: 0.45 ± 0.019	2.418SerTyr: 2.418 ± 0.048	0.005SerXaa: 0.005 ± 0.002
Thr	3.732ThrAla: 3.732 ± 0.073	0.478ThrCys: 0.478 ± 0.017	2.844ThrAsp: 2.844 ± 0.054	3.382ThrGlu: 3.382 ± 0.043	2.688ThrPhe: 2.688 ± 0.051	3.996ThrGly: 3.996 ± 0.068	1.238ThrHis: 1.238 ± 0.029	4.489ThrIle: 4.489 ± 0.069	3.14ThrLys: 3.14 ± 0.051	6.192ThrLeu: 6.192 ± 0.083	1.326ThrMet: 1.326 ± 0.033	2.248ThrAsn: 2.248 ± 0.053	2.205ThrPro: 2.205 ± 0.047	1.763ThrGln: 1.763 ± 0.039	1.741ThrArg: 1.741 ± 0.033	3.386ThrSer: 3.386 ± 0.059	3.002ThrThr: 3.002 ± 0.063	3.966ThrVal: 3.966 ± 0.066	0.41ThrTrp: 0.41 ± 0.02	2.242ThrTyr: 2.242 ± 0.047	0.004ThrXaa: 0.004 ± 0.002
Val	4.489ValAla: 4.489 ± 0.065	0.672ValCys: 0.672 ± 0.021	3.971ValAsp: 3.971 ± 0.052	4.524ValGlu: 4.524 ± 0.067	3.082ValPhe: 3.082 ± 0.051	4.283ValGly: 4.283 ± 0.059	1.104ValHis: 1.104 ± 0.03	5.96ValIle: 5.96 ± 0.061	4.849ValLys: 4.849 ± 0.06	6.475ValLeu: 6.475 ± 0.076	1.87ValMet: 1.87 ± 0.038	3.228ValAsn: 3.228 ± 0.049	2.171ValPro: 2.171 ± 0.044	1.749ValGln: 1.749 ± 0.041	2.112ValArg: 2.112 ± 0.04	4.568ValSer: 4.568 ± 0.059	3.875ValThr: 3.875 ± 0.075	4.8ValVal: 4.8 ± 0.068	0.441ValTrp: 0.441 ± 0.017	2.416ValTyr: 2.416 ± 0.047	0.006ValXaa: 0.006 ± 0.003
Trp	0.456TrpAla: 0.456 ± 0.018	0.079TrpCys: 0.079 ± 0.008	0.4TrpAsp: 0.4 ± 0.017	0.41TrpGlu: 0.41 ± 0.018	0.381TrpPhe: 0.381 ± 0.015	0.568TrpGly: 0.568 ± 0.022	0.223TrpHis: 0.223 ± 0.013	0.692TrpIle: 0.692 ± 0.023	0.42TrpLys: 0.42 ± 0.019	0.766TrpLeu: 0.766 ± 0.023	0.257TrpMet: 0.257 ± 0.014	0.414TrpAsn: 0.414 ± 0.018	0.191TrpPro: 0.191 ± 0.01	0.345TrpGln: 0.345 ± 0.016	0.298TrpArg: 0.298 ± 0.016	0.448TrpSer: 0.448 ± 0.02	0.42TrpThr: 0.42 ± 0.02	0.473TrpVal: 0.473 ± 0.019	0.077TrpTrp: 0.077 ± 0.008	0.299TrpTyr: 0.299 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.514TyrAla: 2.514 ± 0.047	0.479TyrCys: 0.479 ± 0.02	2.594TyrAsp: 2.594 ± 0.048	3.087TyrGlu: 3.087 ± 0.053	2.123TyrPhe: 2.123 ± 0.042	2.598TyrGly: 2.598 ± 0.047	0.831TyrHis: 0.831 ± 0.026	2.97TyrIle: 2.97 ± 0.049	2.844TyrLys: 2.844 ± 0.047	3.835TyrLeu: 3.835 ± 0.054	0.983TyrMet: 0.983 ± 0.024	2.027TyrAsn: 2.027 ± 0.042	1.323TyrPro: 1.323 ± 0.028	1.359TyrGln: 1.359 ± 0.029	1.618TyrArg: 1.618 ± 0.034	2.317TyrSer: 2.317 ± 0.045	2.095TyrThr: 2.095 ± 0.051	2.341TyrVal: 2.341 ± 0.04	0.321TyrTrp: 0.321 ± 0.015	1.885TyrTyr: 1.885 ± 0.044	0.002TyrXaa: 0.002 ± 0.001
Xaa	0.004XaaAla: 0.004 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.005XaaAsp: 0.005 ± 0.002	0.003XaaGlu: 0.003 ± 0.001	0.005XaaPhe: 0.005 ± 0.002	0.006XaaGly: 0.006 ± 0.002	0.001XaaHis: 0.001 ± 0.001	0.008XaaIle: 0.008 ± 0.003	0.003XaaLys: 0.003 ± 0.002	0.003XaaLeu: 0.003 ± 0.002	0.001XaaMet: 0.001 ± 0.001	0.005XaaAsn: 0.005 ± 0.002	0.002XaaPro: 0.002 ± 0.001	0.004XaaGln: 0.004 ± 0.002	0.002XaaArg: 0.002 ± 0.001	0.005XaaSer: 0.005 ± 0.002	0.003XaaThr: 0.003 ± 0.001	0.004XaaVal: 0.004 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4633 proteins (1463251 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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