Sphingobacterium alimentarium

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3405 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R3VM62|A0A4R3VM62_9SPHI Uncharacterized protein YbbC (DUF1343 family) OS=Sphingobacterium alimentarium OX=797292 GN=EDC17_10833 PE=4 SV=1
MM1 pKa = 7.34NNEE4 pKa = 3.99DD5 pKa = 3.45LNYY8 pKa = 8.24YY9 pKa = 10.79NRR11 pKa = 11.84MIANFHH17 pKa = 6.83EE18 pKa = 4.59YY19 pKa = 9.7IQDD22 pKa = 3.75RR23 pKa = 11.84EE24 pKa = 4.3HH25 pKa = 6.86EE26 pKa = 4.37GAKK29 pKa = 10.27SIMSFIVSTHH39 pKa = 5.22VDD41 pKa = 3.43NIVAFEE47 pKa = 3.95ATKK50 pKa = 10.21TDD52 pKa = 3.24SSEE55 pKa = 4.49SLYY58 pKa = 10.7QQDD61 pKa = 4.18KK62 pKa = 10.77LNEE65 pKa = 4.15ITSLLQSVQQSYY77 pKa = 9.4PYY79 pKa = 10.23YY80 pKa = 10.64FPALDD85 pKa = 4.44LQDD88 pKa = 4.13LDD90 pKa = 4.5AIKK93 pKa = 10.51VYY95 pKa = 9.22HH96 pKa = 5.74QKK98 pKa = 11.24YY99 pKa = 8.86MDD101 pKa = 4.19EE102 pKa = 4.2LLEE105 pKa = 5.67DD106 pKa = 3.7EE107 pKa = 5.64DD108 pKa = 4.2FARR111 pKa = 11.84DD112 pKa = 3.45MGVEE116 pKa = 3.86PRR118 pKa = 11.84KK119 pKa = 8.14TVSIYY124 pKa = 11.13DD125 pKa = 4.52DD126 pKa = 5.67DD127 pKa = 7.08DD128 pKa = 6.26DD129 pKa = 6.49DD130 pKa = 5.51YY131 pKa = 11.83EE132 pKa = 4.06IAEE135 pKa = 4.17YY136 pKa = 11.04DD137 pKa = 3.44EE138 pKa = 5.53DD139 pKa = 5.73AEE141 pKa = 4.92LDD143 pKa = 3.39MMFPNRR149 pKa = 11.84EE150 pKa = 4.25DD151 pKa = 3.85DD152 pKa = 5.35DD153 pKa = 4.02NWDD156 pKa = 3.75DD157 pKa = 3.82FF158 pKa = 4.47

Molecular weight: 18.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3VYK1|A0A4R3VYK1_9SPHI Conjugative transposon protein TraO OS=Sphingobacterium alimentarium OX=797292 GN=EDC17_100510 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.39KK6 pKa = 9.62RR7 pKa = 11.84KK8 pKa = 6.72RR9 pKa = 11.84HH10 pKa = 5.17KK11 pKa = 10.05MATHH15 pKa = 6.02KK16 pKa = 10.34RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.26NRR25 pKa = 11.84HH26 pKa = 4.71KK27 pKa = 10.87KK28 pKa = 9.36KK29 pKa = 10.77

Molecular weight: 3.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3405

0

3405

1064945

25

1814

312.8

35.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.909 ± 0.038

0.735 ± 0.013

5.448 ± 0.034

6.345 ± 0.043

4.92 ± 0.029

6.372 ± 0.044

1.955 ± 0.017

7.826 ± 0.04

7.252 ± 0.04

9.521 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.315 ± 0.02

5.755 ± 0.04

3.447 ± 0.024

3.876 ± 0.023

3.976 ± 0.028

6.402 ± 0.031

5.403 ± 0.03

6.236 ± 0.034

1.097 ± 0.014

4.21 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski