Flavobacterium commune
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3082 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D9P7R4|A0A1D9P7R4_9FLAO 50S ribosomal protein L15 OS=Flavobacterium commune OX=1306519 GN=rplO PE=3 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.16 KK3 pKa = 8.74 IQHH6 pKa = 7.1 IITILCLLFLLPCCEE21 pKa = 4.1 NDD23 pKa = 3.87 GGDD26 pKa = 4.59 SKK28 pKa = 11.33 ISTQKK33 pKa = 10.67 GALPNIQKK41 pKa = 10.58 LEE43 pKa = 4.06 NSDD46 pKa = 3.84 SFIDD50 pKa = 4.8 LVAVQNNQEE59 pKa = 4.02 INLGFSVDD67 pKa = 3.78 LAIGKK72 pKa = 9.42 ISSMDD77 pKa = 3.47 IIGLYY82 pKa = 9.73 IKK84 pKa = 10.66 SDD86 pKa = 3.28 GTIIKK91 pKa = 8.53 ATLASNITSFPVSFNLNRR109 pKa = 11.84 NDD111 pKa = 4.7 LYY113 pKa = 11.46 DD114 pKa = 3.66 AFEE117 pKa = 4.56 NLNSSEE123 pKa = 4.42 DD124 pKa = 3.77 FATGDD129 pKa = 3.44 QLIISADD136 pKa = 3.1 ITLKK140 pKa = 10.95 DD141 pKa = 3.53 GTVLKK146 pKa = 10.71 IMNDD150 pKa = 3.3 DD151 pKa = 3.52 GTNNFSSNIATSNLYY166 pKa = 10.36 KK167 pKa = 10.55 VFQTYY172 pKa = 9.8 NVSCPSYY179 pKa = 10.82 LAGTYY184 pKa = 10.14 NYY186 pKa = 8.05 STTNIGDD193 pKa = 3.56 GSYY196 pKa = 8.64 FTSDD200 pKa = 3.05 VFTGTVTFEE209 pKa = 4.08 DD210 pKa = 3.4 QGGGVYY216 pKa = 10.15 IISDD220 pKa = 3.92 GTFGGYY226 pKa = 6.78 TALYY230 pKa = 9.73 DD231 pKa = 3.48 EE232 pKa = 4.57 TAIGVEE238 pKa = 4.84 LKK240 pKa = 10.51 DD241 pKa = 3.11 ICNQISFQGSNQYY254 pKa = 11.38 GDD256 pKa = 3.73 TFTMSNLVVNGNKK269 pKa = 8.4 LTFHH273 pKa = 7.09 WEE275 pKa = 3.85 TSFGEE280 pKa = 4.38 FGDD283 pKa = 4.0 TTLTRR288 pKa = 11.84 TDD290 pKa = 3.08 NTNWPNLTLL299 pKa = 4.07
Molecular weight: 32.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.784
IPC2_protein 4.024
IPC_protein 4.024
Toseland 3.795
ProMoST 4.19
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.961
Rodwell 3.846
Grimsley 3.706
Solomon 4.012
Lehninger 3.973
Nozaki 4.126
DTASelect 4.406
Thurlkill 3.859
EMBOSS 3.973
Sillero 4.151
Patrickios 1.24
IPC_peptide 4.012
IPC2_peptide 4.126
IPC2.peptide.svr19 4.007
Protein with the highest isoelectric point:
>tr|A0A1D9P6S7|A0A1D9P6S7_9FLAO Transcriptional regulator OS=Flavobacterium commune OX=1306519 GN=BIW12_01915 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.91 HH17 pKa = 4.39 GFMDD21 pKa = 4.5 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.19 GRR40 pKa = 11.84 HH41 pKa = 5.41 KK42 pKa = 10.14 LTVSSEE48 pKa = 3.92 PRR50 pKa = 11.84 HH51 pKa = 5.77 KK52 pKa = 10.61 KK53 pKa = 9.84
Molecular weight: 6.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.063
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3082
0
3082
1113438
42
3917
361.3
40.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.704 ± 0.05
0.781 ± 0.017
5.303 ± 0.029
6.345 ± 0.045
5.217 ± 0.034
6.379 ± 0.041
1.648 ± 0.021
8.027 ± 0.046
8.043 ± 0.052
9.003 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.162 ± 0.024
6.498 ± 0.045
3.43 ± 0.023
3.466 ± 0.023
3.143 ± 0.027
6.502 ± 0.037
5.912 ± 0.079
6.211 ± 0.043
1.137 ± 0.021
4.09 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here