Pedobacter nutrimenti
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4924 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A318UGF7|A0A318UGF7_9SPHI Acetyltransferase (GNAT) family protein OS=Pedobacter nutrimenti OX=1241337 GN=B0O44_10315 PE=4 SV=1
MM1 pKa = 7.59 LSYY4 pKa = 9.78 MQIIPPMDD12 pKa = 5.18 DD13 pKa = 3.98 DD14 pKa = 5.67 KK15 pKa = 11.52 MDD17 pKa = 3.52 GVLFIAALPVFYY29 pKa = 10.08 FIIAQTAKK37 pKa = 9.67 RR38 pKa = 11.84 CHH40 pKa = 6.52 DD41 pKa = 4.22 RR42 pKa = 11.84 GDD44 pKa = 3.81 SGWWMIVPLYY54 pKa = 9.51 GFWMLFADD62 pKa = 5.49 GDD64 pKa = 3.91 IGDD67 pKa = 4.57 NEE69 pKa = 4.42 YY70 pKa = 11.31 GPNPKK75 pKa = 10.05 GLYY78 pKa = 10.07 YY79 pKa = 10.47 EE80 pKa = 5.37 FEE82 pKa = 4.49 DD83 pKa = 4.35 HH84 pKa = 6.96 NSPSPIDD91 pKa = 3.59 QVDD94 pKa = 3.16 GDD96 pKa = 3.93 GRR98 pKa = 11.84 III100 pKa = 4.09
Molecular weight: 11.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.999
IPC_protein 3.973
Toseland 3.745
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.961
Rodwell 3.795
Grimsley 3.656
Solomon 3.973
Lehninger 3.935
Nozaki 4.113
DTASelect 4.406
Thurlkill 3.821
EMBOSS 3.973
Sillero 4.101
Patrickios 1.964
IPC_peptide 3.961
IPC2_peptide 4.075
IPC2.peptide.svr19 3.976
Protein with the highest isoelectric point:
>tr|A0A318U6F1|A0A318U6F1_9SPHI Uncharacterized protein OS=Pedobacter nutrimenti OX=1241337 GN=B0O44_11214 PE=4 SV=1
MM1 pKa = 7.78 SDD3 pKa = 2.66 GHH5 pKa = 6.55 LAFMIGLLGSVHH17 pKa = 7.11 CIGMCGPLAFAVPSVKK33 pKa = 9.64 TGRR36 pKa = 11.84 VYY38 pKa = 11.39 LFTDD42 pKa = 3.26 KK43 pKa = 11.0 VLYY46 pKa = 10.12 QLGRR50 pKa = 11.84 MISYY54 pKa = 10.23 SVLGACIGGIGRR66 pKa = 11.84 QLWLSGLQQGISVASGLLILAAACSRR92 pKa = 11.84 IFKK95 pKa = 10.51 LSGVNRR101 pKa = 11.84 TPAFLLQPFHH111 pKa = 7.13 RR112 pKa = 11.84 LLGAALKK119 pKa = 10.51 HH120 pKa = 5.82 KK121 pKa = 10.31 ANHH124 pKa = 6.58 LIIGIINGLLPCGFVYY140 pKa = 10.51 LALAGAVNTGSIPAASAYY158 pKa = 7.8 MFWFGAGTAPLMLLATIGVGFSGKK182 pKa = 7.56 VLRR185 pKa = 11.84 GRR187 pKa = 11.84 INRR190 pKa = 11.84 MVPYY194 pKa = 10.93 LMLFLGIWFILRR206 pKa = 11.84 GMDD209 pKa = 4.23 LNIPYY214 pKa = 10.17 LSPSKK219 pKa = 10.05 PSSGVAEE226 pKa = 4.4 CRR228 pKa = 3.51
Molecular weight: 24.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.604
IPC_protein 9.955
Toseland 10.014
ProMoST 9.955
Dawson 10.277
Bjellqvist 10.043
Wikipedia 10.496
Rodwell 10.54
Grimsley 10.365
Solomon 10.321
Lehninger 10.277
Nozaki 10.145
DTASelect 10.014
Thurlkill 10.116
EMBOSS 10.452
Sillero 10.218
Patrickios 9.94
IPC_peptide 10.306
IPC2_peptide 9.282
IPC2.peptide.svr19 8.406
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4924
0
4924
1744692
39
5029
354.3
39.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.404 ± 0.037
0.729 ± 0.01
5.031 ± 0.019
5.609 ± 0.039
4.992 ± 0.027
6.888 ± 0.036
1.758 ± 0.018
6.929 ± 0.035
7.387 ± 0.04
10.02 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.255 ± 0.015
5.568 ± 0.036
3.795 ± 0.022
3.991 ± 0.023
3.84 ± 0.024
6.713 ± 0.031
5.574 ± 0.051
6.236 ± 0.028
1.112 ± 0.013
4.17 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here