Amino acid dipepetide frequency for Pedobacter nutrimenti

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.793AlaAla: 6.793 ± 0.088
0.62AlaCys: 0.62 ± 0.019
4.071AlaAsp: 4.071 ± 0.059
4.37AlaGlu: 4.37 ± 0.057
3.604AlaPhe: 3.604 ± 0.052
5.886AlaGly: 5.886 ± 0.086
1.166AlaHis: 1.166 ± 0.025
4.937AlaIle: 4.937 ± 0.064
4.651AlaLys: 4.651 ± 0.059
7.54AlaLeu: 7.54 ± 0.076
1.673AlaMet: 1.673 ± 0.035
3.369AlaAsn: 3.369 ± 0.055
2.345AlaPro: 2.345 ± 0.038
3.146AlaGln: 3.146 ± 0.044
2.487AlaArg: 2.487 ± 0.037
4.836AlaSer: 4.836 ± 0.06
3.802AlaThr: 3.802 ± 0.089
4.833AlaVal: 4.833 ± 0.067
0.76AlaTrp: 0.76 ± 0.025
3.154AlaTyr: 3.154 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.481CysAla: 0.481 ± 0.019
0.113CysCys: 0.113 ± 0.008
0.308CysAsp: 0.308 ± 0.014
0.314CysGlu: 0.314 ± 0.014
0.456CysPhe: 0.456 ± 0.017
0.553CysGly: 0.553 ± 0.02
0.159CysHis: 0.159 ± 0.01
0.6CysIle: 0.6 ± 0.018
0.506CysLys: 0.506 ± 0.015
0.786CysLeu: 0.786 ± 0.021
0.177CysMet: 0.177 ± 0.01
0.347CysAsn: 0.347 ± 0.016
0.292CysPro: 0.292 ± 0.012
0.193CysGln: 0.193 ± 0.011
0.291CysArg: 0.291 ± 0.013
0.547CysSer: 0.547 ± 0.018
0.412CysThr: 0.412 ± 0.017
0.391CysVal: 0.391 ± 0.014
0.088CysTrp: 0.088 ± 0.009
0.273CysTyr: 0.273 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.646AspAla: 3.646 ± 0.054
0.344AspCys: 0.344 ± 0.011
2.042AspAsp: 2.042 ± 0.039
2.706AspGlu: 2.706 ± 0.043
3.066AspPhe: 3.066 ± 0.041
3.453AspGly: 3.453 ± 0.051
1.087AspHis: 1.087 ± 0.025
3.437AspIle: 3.437 ± 0.052
3.673AspLys: 3.673 ± 0.058
5.318AspLeu: 5.318 ± 0.053
1.101AspMet: 1.101 ± 0.028
2.39AspAsn: 2.39 ± 0.041
2.358AspPro: 2.358 ± 0.045
2.401AspGln: 2.401 ± 0.041
2.138AspArg: 2.138 ± 0.037
2.499AspSer: 2.499 ± 0.044
2.175AspThr: 2.175 ± 0.041
3.205AspVal: 3.205 ± 0.047
0.694AspTrp: 0.694 ± 0.021
2.571AspTyr: 2.571 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
3.973GluAla: 3.973 ± 0.058
0.287GluCys: 0.287 ± 0.013
2.572GluAsp: 2.572 ± 0.042
3.714GluGlu: 3.714 ± 0.063
2.326GluPhe: 2.326 ± 0.041
3.31GluGly: 3.31 ± 0.048
1.162GluHis: 1.162 ± 0.027
4.29GluIle: 4.29 ± 0.056
5.193GluLys: 5.193 ± 0.066
6.005GluLeu: 6.005 ± 0.068
1.37GluMet: 1.37 ± 0.028
3.429GluAsn: 3.429 ± 0.051
1.534GluPro: 1.534 ± 0.03
2.825GluGln: 2.825 ± 0.047
2.41GluArg: 2.41 ± 0.042
2.829GluSer: 2.829 ± 0.047
2.67GluThr: 2.67 ± 0.039
3.707GluVal: 3.707 ± 0.048
0.573GluTrp: 0.573 ± 0.018
1.915GluTyr: 1.915 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
3.197PheAla: 3.197 ± 0.051
0.49PheCys: 0.49 ± 0.017
2.702PheAsp: 2.702 ± 0.048
2.775PheGlu: 2.775 ± 0.043
2.661PhePhe: 2.661 ± 0.05
3.403PheGly: 3.403 ± 0.055
0.756PheHis: 0.756 ± 0.022
3.481PheIle: 3.481 ± 0.056
3.705PheLys: 3.705 ± 0.048
4.673PheLeu: 4.673 ± 0.069
1.142PheMet: 1.142 ± 0.027
3.147PheAsn: 3.147 ± 0.052
1.686PhePro: 1.686 ± 0.034
1.486PheGln: 1.486 ± 0.03
1.86PheArg: 1.86 ± 0.039
4.274PheSer: 4.274 ± 0.051
2.911PheThr: 2.911 ± 0.05
2.779PheVal: 2.779 ± 0.043
0.589PheTrp: 0.589 ± 0.019
2.202PheTyr: 2.202 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
4.835GlyAla: 4.835 ± 0.068
0.542GlyCys: 0.542 ± 0.02
3.044GlyAsp: 3.044 ± 0.053
3.13GlyGlu: 3.13 ± 0.044
3.682GlyPhe: 3.682 ± 0.05
4.859GlyGly: 4.859 ± 0.078
1.204GlyHis: 1.204 ± 0.029
5.339GlyIle: 5.339 ± 0.061
5.55GlyLys: 5.55 ± 0.061
7.002GlyLeu: 7.002 ± 0.075
1.682GlyMet: 1.682 ± 0.031
3.841GlyAsn: 3.841 ± 0.068
1.771GlyPro: 1.771 ± 0.039
2.672GlyGln: 2.672 ± 0.041
2.523GlyArg: 2.523 ± 0.041
4.559GlySer: 4.559 ± 0.068
4.452GlyThr: 4.452 ± 0.094
4.198GlyVal: 4.198 ± 0.05
0.873GlyTrp: 0.873 ± 0.025
3.119GlyTyr: 3.119 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
1.051HisAla: 1.051 ± 0.027
0.181HisCys: 0.181 ± 0.011
0.745HisAsp: 0.745 ± 0.022
0.887HisGlu: 0.887 ± 0.023
1.185HisPhe: 1.185 ± 0.025
1.017HisGly: 1.017 ± 0.026
0.523HisHis: 0.523 ± 0.02
1.24HisIle: 1.24 ± 0.027
1.146HisLys: 1.146 ± 0.025
1.991HisLeu: 1.991 ± 0.037
0.384HisMet: 0.384 ± 0.013
0.786HisAsn: 0.786 ± 0.021
0.946HisPro: 0.946 ± 0.027
0.907HisGln: 0.907 ± 0.024
0.73HisArg: 0.73 ± 0.021
1.078HisSer: 1.078 ± 0.028
0.898HisThr: 0.898 ± 0.022
0.863HisVal: 0.863 ± 0.023
0.21HisTrp: 0.21 ± 0.012
0.812HisTyr: 0.812 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.403IleAla: 5.403 ± 0.059
0.657IleCys: 0.657 ± 0.02
3.806IleAsp: 3.806 ± 0.05
4.174IleGlu: 4.174 ± 0.053
3.086IlePhe: 3.086 ± 0.055
4.757IleGly: 4.757 ± 0.06
1.233IleHis: 1.233 ± 0.029
4.293IleIle: 4.293 ± 0.082
4.922IleLys: 4.922 ± 0.06
6.449IleLeu: 6.449 ± 0.078
1.284IleMet: 1.284 ± 0.026
3.877IleAsn: 3.877 ± 0.054
3.041IlePro: 3.041 ± 0.043
2.442IleGln: 2.442 ± 0.036
3.191IleArg: 3.191 ± 0.043
5.361IleSer: 5.361 ± 0.065
4.09IleThr: 4.09 ± 0.066
3.943IleVal: 3.943 ± 0.048
0.661IleTrp: 0.661 ± 0.022
2.621IleTyr: 2.621 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
5.4LysAla: 5.4 ± 0.063
0.293LysCys: 0.293 ± 0.013
4.366LysAsp: 4.366 ± 0.062
5.117LysGlu: 5.117 ± 0.065
2.657LysPhe: 2.657 ± 0.044
4.852LysGly: 4.852 ± 0.058
1.199LysHis: 1.199 ± 0.025
5.394LysIle: 5.394 ± 0.063
6.143LysLys: 6.143 ± 0.088
6.402LysLeu: 6.402 ± 0.069
1.978LysMet: 1.978 ± 0.031
4.675LysAsn: 4.675 ± 0.057
2.68LysPro: 2.68 ± 0.041
2.929LysGln: 2.929 ± 0.049
2.759LysArg: 2.759 ± 0.041
4.28LysSer: 4.28 ± 0.055
4.569LysThr: 4.569 ± 0.057
4.505LysVal: 4.505 ± 0.061
0.777LysTrp: 0.777 ± 0.021
2.892LysTyr: 2.892 ± 0.043
0.0LysXaa: 0.0 ± 0.0
Leu
6.685LeuAla: 6.685 ± 0.079
0.844LeuCys: 0.844 ± 0.023
4.827LeuAsp: 4.827 ± 0.054
5.338LeuGlu: 5.338 ± 0.076
5.122LeuPhe: 5.122 ± 0.076
6.054LeuGly: 6.054 ± 0.069
1.695LeuHis: 1.695 ± 0.039
6.747LeuIle: 6.747 ± 0.085
8.368LeuLys: 8.368 ± 0.074
10.492LeuLeu: 10.492 ± 0.111
2.389LeuMet: 2.389 ± 0.039
6.031LeuAsn: 6.031 ± 0.064
4.178LeuPro: 4.178 ± 0.053
4.009LeuGln: 4.009 ± 0.058
3.765LeuArg: 3.765 ± 0.053
8.188LeuSer: 8.188 ± 0.086
5.361LeuThr: 5.361 ± 0.081
5.6LeuVal: 5.6 ± 0.065
1.019LeuTrp: 1.019 ± 0.029
3.488LeuTyr: 3.488 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
1.779MetAla: 1.779 ± 0.034
0.122MetCys: 0.122 ± 0.008
1.231MetAsp: 1.231 ± 0.026
1.463MetGlu: 1.463 ± 0.033
0.785MetPhe: 0.785 ± 0.022
1.572MetGly: 1.572 ± 0.029
0.386MetHis: 0.386 ± 0.017
1.511MetIle: 1.511 ± 0.03
2.05MetLys: 2.05 ± 0.035
2.144MetLeu: 2.144 ± 0.037
0.649MetMet: 0.649 ± 0.02
1.335MetAsn: 1.335 ± 0.031
0.996MetPro: 0.996 ± 0.024
0.89MetGln: 0.89 ± 0.026
0.852MetArg: 0.852 ± 0.022
1.349MetSer: 1.349 ± 0.027
1.194MetThr: 1.194 ± 0.027
1.416MetVal: 1.416 ± 0.031
0.155MetTrp: 0.155 ± 0.009
0.668MetTyr: 0.668 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
4.096AsnAla: 4.096 ± 0.068
0.372AsnCys: 0.372 ± 0.014
2.533AsnAsp: 2.533 ± 0.045
2.776AsnGlu: 2.776 ± 0.047
2.773AsnPhe: 2.773 ± 0.043
4.154AsnGly: 4.154 ± 0.055
0.967AsnHis: 0.967 ± 0.025
3.945AsnIle: 3.945 ± 0.058
3.685AsnLys: 3.685 ± 0.047
5.278AsnLeu: 5.278 ± 0.056
1.15AsnMet: 1.15 ± 0.027
3.206AsnAsn: 3.206 ± 0.057
2.787AsnPro: 2.787 ± 0.046
2.168AsnGln: 2.168 ± 0.041
2.226AsnArg: 2.226 ± 0.036
3.487AsnSer: 3.487 ± 0.053
3.423AsnThr: 3.423 ± 0.065
3.139AsnVal: 3.139 ± 0.049
0.757AsnTrp: 0.757 ± 0.02
2.755AsnTyr: 2.755 ± 0.049
0.0AsnXaa: 0.0 ± 0.0
Pro
3.359ProAla: 3.359 ± 0.068
0.203ProCys: 0.203 ± 0.012
2.459ProAsp: 2.459 ± 0.036
2.85ProGlu: 2.85 ± 0.039
1.917ProPhe: 1.917 ± 0.031
3.073ProGly: 3.073 ± 0.058
0.565ProHis: 0.565 ± 0.018
2.023ProIle: 2.023 ± 0.037
2.267ProLys: 2.267 ± 0.04
3.649ProLeu: 3.649 ± 0.046
0.761ProMet: 0.761 ± 0.023
1.821ProAsn: 1.821 ± 0.035
0.972ProPro: 0.972 ± 0.027
1.463ProGln: 1.463 ± 0.033
1.062ProArg: 1.062 ± 0.024
2.443ProSer: 2.443 ± 0.041
1.679ProThr: 1.679 ± 0.048
3.497ProVal: 3.497 ± 0.062
0.359ProTrp: 0.359 ± 0.015
1.529ProTyr: 1.529 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.003GlnAla: 3.003 ± 0.048
0.177GlnCys: 0.177 ± 0.01
1.908GlnAsp: 1.908 ± 0.044
2.334GlnGlu: 2.334 ± 0.041
1.658GlnPhe: 1.658 ± 0.036
2.359GlnGly: 2.359 ± 0.039
0.781GlnHis: 0.781 ± 0.023
2.687GlnIle: 2.687 ± 0.04
3.007GlnLys: 3.007 ± 0.053
4.164GlnLeu: 4.164 ± 0.055
0.936GlnMet: 0.936 ± 0.025
2.274GlnAsn: 2.274 ± 0.04
1.417GlnPro: 1.417 ± 0.031
2.419GlnGln: 2.419 ± 0.049
1.537GlnArg: 1.537 ± 0.03
2.461GlnSer: 2.461 ± 0.042
2.215GlnThr: 2.215 ± 0.037
2.64GlnVal: 2.64 ± 0.038
0.451GlnTrp: 0.451 ± 0.017
1.485GlnTyr: 1.485 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
2.471ArgAla: 2.471 ± 0.042
0.22ArgCys: 0.22 ± 0.013
1.789ArgAsp: 1.789 ± 0.032
2.218ArgGlu: 2.218 ± 0.04
2.184ArgPhe: 2.184 ± 0.037
2.087ArgGly: 2.087 ± 0.041
0.661ArgHis: 0.661 ± 0.02
3.118ArgIle: 3.118 ± 0.047
2.978ArgLys: 2.978 ± 0.043
4.006ArgLeu: 4.006 ± 0.051
1.037ArgMet: 1.037 ± 0.025
2.251ArgAsn: 2.251 ± 0.04
1.413ArgPro: 1.413 ± 0.031
1.454ArgGln: 1.454 ± 0.033
1.462ArgArg: 1.462 ± 0.029
2.453ArgSer: 2.453 ± 0.04
2.104ArgThr: 2.104 ± 0.035
2.217ArgVal: 2.217 ± 0.038
0.472ArgTrp: 0.472 ± 0.017
1.801ArgTyr: 1.801 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
5.174SerAla: 5.174 ± 0.071
0.577SerCys: 0.577 ± 0.019
3.091SerAsp: 3.091 ± 0.048
3.04SerGlu: 3.04 ± 0.047
3.848SerPhe: 3.848 ± 0.055
5.599SerGly: 5.599 ± 0.074
1.047SerHis: 1.047 ± 0.025
4.764SerIle: 4.764 ± 0.057
4.342SerLys: 4.342 ± 0.048
6.816SerLeu: 6.816 ± 0.072
1.353SerMet: 1.353 ± 0.028
3.42SerAsn: 3.42 ± 0.053
2.604SerPro: 2.604 ± 0.041
2.106SerGln: 2.106 ± 0.036
2.701SerArg: 2.701 ± 0.042
4.803SerSer: 4.803 ± 0.061
3.698SerThr: 3.698 ± 0.059
4.341SerVal: 4.341 ± 0.057
0.785SerTrp: 0.785 ± 0.025
3.02SerTyr: 3.02 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
4.778ThrAla: 4.778 ± 0.111
0.331ThrCys: 0.331 ± 0.013
3.237ThrAsp: 3.237 ± 0.057
3.178ThrGlu: 3.178 ± 0.043
2.716ThrPhe: 2.716 ± 0.044
4.875ThrGly: 4.875 ± 0.089
0.833ThrHis: 0.833 ± 0.024
3.892ThrIle: 3.892 ± 0.063
3.202ThrLys: 3.202 ± 0.041
5.473ThrLeu: 5.473 ± 0.083
0.982ThrMet: 0.982 ± 0.026
2.634ThrAsn: 2.634 ± 0.054
2.277ThrPro: 2.277 ± 0.06
1.787ThrGln: 1.787 ± 0.034
1.868ThrArg: 1.868 ± 0.031
3.556ThrSer: 3.556 ± 0.07
3.221ThrThr: 3.221 ± 0.089
3.914ThrVal: 3.914 ± 0.08
0.565ThrTrp: 0.565 ± 0.02
2.424ThrTyr: 2.424 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
4.224ValAla: 4.224 ± 0.062
0.518ValCys: 0.518 ± 0.02
2.856ValAsp: 2.856 ± 0.046
3.191ValGlu: 3.191 ± 0.045
3.316ValPhe: 3.316 ± 0.051
3.422ValGly: 3.422 ± 0.053
1.078ValHis: 1.078 ± 0.024
4.366ValIle: 4.366 ± 0.053
4.723ValLys: 4.723 ± 0.059
6.814ValLeu: 6.814 ± 0.065
1.422ValMet: 1.422 ± 0.031
3.643ValAsn: 3.643 ± 0.062
2.602ValPro: 2.602 ± 0.043
2.329ValGln: 2.329 ± 0.033
2.293ValArg: 2.293 ± 0.039
4.581ValSer: 4.581 ± 0.058
3.519ValThr: 3.519 ± 0.096
4.191ValVal: 4.191 ± 0.067
0.668ValTrp: 0.668 ± 0.024
2.607ValTyr: 2.607 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
0.755TrpAla: 0.755 ± 0.024
0.095TrpCys: 0.095 ± 0.008
0.546TrpAsp: 0.546 ± 0.017
0.632TrpGlu: 0.632 ± 0.021
0.554TrpPhe: 0.554 ± 0.017
0.75TrpGly: 0.75 ± 0.02
0.198TrpHis: 0.198 ± 0.012
0.746TrpIle: 0.746 ± 0.022
0.849TrpLys: 0.849 ± 0.025
1.157TrpLeu: 1.157 ± 0.028
0.32TrpMet: 0.32 ± 0.015
0.649TrpAsn: 0.649 ± 0.021
0.327TrpPro: 0.327 ± 0.015
0.465TrpGln: 0.465 ± 0.018
0.416TrpArg: 0.416 ± 0.016
0.718TrpSer: 0.718 ± 0.023
0.655TrpThr: 0.655 ± 0.022
0.651TrpVal: 0.651 ± 0.024
0.166TrpTrp: 0.166 ± 0.01
0.472TrpTyr: 0.472 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.94TyrAla: 2.94 ± 0.047
0.301TyrCys: 0.301 ± 0.012
2.173TyrAsp: 2.173 ± 0.04
1.885TyrGlu: 1.885 ± 0.033
2.322TyrPhe: 2.322 ± 0.039
2.839TyrGly: 2.839 ± 0.047
0.878TyrHis: 0.878 ± 0.024
2.486TyrIle: 2.486 ± 0.04
2.905TyrLys: 2.905 ± 0.045
4.04TyrLeu: 4.04 ± 0.061
0.757TyrMet: 0.757 ± 0.023
2.552TyrAsn: 2.552 ± 0.047
1.726TyrPro: 1.726 ± 0.036
1.824TyrGln: 1.824 ± 0.029
1.828TyrArg: 1.828 ± 0.034
2.825TyrSer: 2.825 ± 0.048
2.696TyrThr: 2.696 ± 0.055
2.329TyrVal: 2.329 ± 0.039
0.5TyrTrp: 0.5 ± 0.022
1.894TyrTyr: 1.894 ± 0.04
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4924 proteins (1744692 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski