Halobacterium phage phiH (Bacteriophage phi-H)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Myohalovirus; Halobacterium virus phiH

Average proteome isoelectric point is 4.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G1ZKX6|A0A3G1ZKX6_BPPHH Uncharacterized protein OS=Halobacterium phage phiH OX=169684 GN=PhiH1_205 PE=4 SV=1
MM1 pKa = 7.06TVEE4 pKa = 3.86NPYY7 pKa = 10.31FEE9 pKa = 4.73EE10 pKa = 4.26VAGGITVGANRR21 pKa = 11.84DD22 pKa = 3.35SWDD25 pKa = 3.27DD26 pKa = 3.33RR27 pKa = 11.84AIEE30 pKa = 4.0VLEE33 pKa = 3.71QRR35 pKa = 11.84MDD37 pKa = 3.86LVQEE41 pKa = 4.27YY42 pKa = 10.41AWAIPNEE49 pKa = 4.17EE50 pKa = 4.5AIEE53 pKa = 4.45TIAEE57 pKa = 4.07HH58 pKa = 6.29APVVEE63 pKa = 4.59VGAGAGYY70 pKa = 8.43WAWCVDD76 pKa = 3.4QLGARR81 pKa = 11.84IVATDD86 pKa = 3.93PEE88 pKa = 4.78PPRR91 pKa = 11.84MDD93 pKa = 3.52TYY95 pKa = 11.42TDD97 pKa = 4.42IITKK101 pKa = 8.65TATEE105 pKa = 4.65AIEE108 pKa = 4.08CARR111 pKa = 11.84EE112 pKa = 3.97IFVDD116 pKa = 5.29GYY118 pKa = 9.0TLFVCWPPYY127 pKa = 9.61DD128 pKa = 4.82DD129 pKa = 5.61PMAADD134 pKa = 3.66AVDD137 pKa = 3.89AFEE140 pKa = 5.62GDD142 pKa = 3.4TLIYY146 pKa = 10.11VGQGRR151 pKa = 11.84CGCTADD157 pKa = 3.75EE158 pKa = 4.14RR159 pKa = 11.84LHH161 pKa = 6.38RR162 pKa = 11.84LLYY165 pKa = 10.31EE166 pKa = 4.16DD167 pKa = 4.02WEE169 pKa = 4.36LTEE172 pKa = 4.38TVAIPTYY179 pKa = 10.7LGFNDD184 pKa = 4.4RR185 pKa = 11.84LEE187 pKa = 4.11VWSRR191 pKa = 3.15

Molecular weight: 21.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G1ZKU6|A0A3G1ZKU6_BPPHH Uncharacterized protein OS=Halobacterium phage phiH OX=169684 GN=PhiH1_260 PE=4 SV=1
MM1 pKa = 7.46ARR3 pKa = 11.84LNRR6 pKa = 11.84HH7 pKa = 5.03PQSNIAYY14 pKa = 8.63AVRR17 pKa = 11.84SLRR20 pKa = 11.84SKK22 pKa = 10.81GQLITNSAPTQNASTDD38 pKa = 3.52ALAVNIDD45 pKa = 3.09HH46 pKa = 7.2RR47 pKa = 11.84AILTVPSYY55 pKa = 10.29RR56 pKa = 11.84SSVRR60 pKa = 11.84VFQRR64 pKa = 11.84SPCLHH69 pKa = 6.46RR70 pKa = 11.84AEE72 pKa = 4.97GLLSPYY78 pKa = 10.01CNRR81 pKa = 11.84GSEE84 pKa = 4.12RR85 pKa = 11.84TNQGNRR91 pKa = 11.84GSAPSKK97 pKa = 9.9ILLPRR102 pKa = 11.84TIADD106 pKa = 3.81PFRR109 pKa = 11.84GGPEE113 pKa = 3.52

Molecular weight: 12.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

18151

32

929

189.1

20.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.107 ± 0.528

0.876 ± 0.118

9.889 ± 0.407

9.553 ± 0.41

2.633 ± 0.105

7.641 ± 0.276

2.204 ± 0.159

4.391 ± 0.184

2.832 ± 0.188

7.829 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.796 ± 0.101

3.069 ± 0.145

4.672 ± 0.303

3.482 ± 0.211

6.11 ± 0.371

6.248 ± 0.373

6.666 ± 0.244

7.019 ± 0.272

1.449 ± 0.108

2.534 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski