Nocardioides guangzhouensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4817 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1XZ50|A0A4V1XZ50_9ACTN Response regulator transcription factor OS=Nocardioides guangzhouensis OX=2497878 GN=EKO23_12210 PE=4 SV=1
MM1 pKa = 7.32STRR4 pKa = 11.84QHH6 pKa = 5.93FAWAAAIAAAAILTPAPASAAPPSNDD32 pKa = 4.0TIDD35 pKa = 3.79GAISASVGFSQVLDD49 pKa = 3.7TTEE52 pKa = 3.95ATTDD56 pKa = 3.48EE57 pKa = 4.54QDD59 pKa = 3.3TQFNEE64 pKa = 4.28TCGAPATDD72 pKa = 3.1ASVWYY77 pKa = 8.12TVAGTGAGVVVDD89 pKa = 5.49VSASSYY95 pKa = 10.21SAGVMVGTGTPDD107 pKa = 3.12NLEE110 pKa = 4.19TVACGPGTVGFLAEE124 pKa = 4.99AGTTYY129 pKa = 10.98YY130 pKa = 11.23VLAFDD135 pKa = 4.62DD136 pKa = 4.43QEE138 pKa = 6.55DD139 pKa = 4.32GTGTGGSLDD148 pKa = 3.32ISFAAAPPPPTAEE161 pKa = 3.69ITVDD165 pKa = 3.27PRR167 pKa = 11.84GTFSSKK173 pKa = 9.73TGIAHH178 pKa = 7.6LSGSYY183 pKa = 9.41TCTDD187 pKa = 3.24ADD189 pKa = 3.82FLEE192 pKa = 4.65VDD194 pKa = 3.92GSVRR198 pKa = 11.84QPVGRR203 pKa = 11.84LAVTGSFFVFDD214 pKa = 4.77EE215 pKa = 5.01GTCDD219 pKa = 3.75GTPHH223 pKa = 6.87PWAADD228 pKa = 3.51VEE230 pKa = 4.3PDD232 pKa = 3.12NGKK235 pKa = 9.54FAGGKK240 pKa = 10.28AMTVTFTFACGPFDD254 pKa = 3.76CAEE257 pKa = 4.27GFAEE261 pKa = 4.25QTVHH265 pKa = 7.36LSGGKK270 pKa = 9.32KK271 pKa = 9.81

Molecular weight: 27.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q4ZKN6|A0A4Q4ZKN6_9ACTN Transcriptional regulator OS=Nocardioides guangzhouensis OX=2497878 GN=EKO23_00380 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4817

0

4817

1549698

28

2324

321.7

34.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.037 ± 0.047

0.763 ± 0.009

6.661 ± 0.028

5.669 ± 0.037

2.794 ± 0.017

9.28 ± 0.033

2.29 ± 0.017

3.169 ± 0.026

1.814 ± 0.026

10.315 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.817 ± 0.012

1.713 ± 0.017

5.748 ± 0.029

2.644 ± 0.021

8.0 ± 0.045

5.09 ± 0.026

6.091 ± 0.032

9.533 ± 0.038

1.592 ± 0.017

1.978 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski