Streptococcus satellite phage Javan749

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZVI6|A0A4D5ZVI6_9VIRU AAA domain-containing protein OS=Streptococcus satellite phage Javan749 OX=2558843 GN=JavanS749_0014 PE=4 SV=1
MM1 pKa = 7.62SLGNKK6 pKa = 9.5QIMADD11 pKa = 3.6NIKK14 pKa = 10.49RR15 pKa = 11.84LLSAKK20 pKa = 9.83GLNPRR25 pKa = 11.84QLAIALDD32 pKa = 4.26FKK34 pKa = 10.84YY35 pKa = 7.62TTVNDD40 pKa = 3.51WVNAKK45 pKa = 9.2TYY47 pKa = 10.43PRR49 pKa = 11.84IDD51 pKa = 4.22KK52 pKa = 10.71IEE54 pKa = 3.81MLANFFNVSKK64 pKa = 11.08SDD66 pKa = 3.61LVEE69 pKa = 4.22NKK71 pKa = 9.96NAEE74 pKa = 4.3TPTTSPIQSIYY85 pKa = 10.88DD86 pKa = 3.27QLTPPRR92 pKa = 11.84QEE94 pKa = 3.78KK95 pKa = 10.64ALTYY99 pKa = 10.35LKK101 pKa = 10.55KK102 pKa = 10.37QLLEE106 pKa = 4.02QKK108 pKa = 10.32NEE110 pKa = 4.21NIVSEE115 pKa = 4.5NIISLDD121 pKa = 3.66DD122 pKa = 3.6YY123 pKa = 11.31RR124 pKa = 11.84EE125 pKa = 4.27SKK127 pKa = 8.86TLPVIGVVTAGNGITQDD144 pKa = 3.61DD145 pKa = 4.02NLNIEE150 pKa = 4.02KK151 pKa = 10.39CFYY154 pKa = 10.27TDD156 pKa = 5.57EE157 pKa = 5.49IPDD160 pKa = 4.38DD161 pKa = 4.3YY162 pKa = 11.82DD163 pKa = 5.57AIAYY167 pKa = 8.54VVGNSMEE174 pKa = 4.18PKK176 pKa = 9.97IKK178 pKa = 10.88NGDD181 pKa = 3.4YY182 pKa = 11.13LFIKK186 pKa = 8.35NTPQVDD192 pKa = 3.97YY193 pKa = 8.82NTIGIFQVDD202 pKa = 3.58GANYY206 pKa = 9.54VKK208 pKa = 10.81KK209 pKa = 10.47LRR211 pKa = 11.84QGYY214 pKa = 9.65LEE216 pKa = 4.73SLNPDD221 pKa = 3.66CADD224 pKa = 3.31IQLDD228 pKa = 3.72EE229 pKa = 5.18SNDD232 pKa = 2.76IRR234 pKa = 11.84TIGEE238 pKa = 3.96VVSIYY243 pKa = 10.62RR244 pKa = 11.84EE245 pKa = 3.88NN246 pKa = 3.2

Molecular weight: 27.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZXX2|A0A4D5ZXX2_9VIRU Glycerate kinase OS=Streptococcus satellite phage Javan749 OX=2558843 GN=JavanS749_0008 PE=4 SV=1
MM1 pKa = 7.12MPKK4 pKa = 10.19SNYY7 pKa = 9.53RR8 pKa = 11.84EE9 pKa = 3.74MRR11 pKa = 11.84FISSLGEE18 pKa = 3.68KK19 pKa = 10.38LLATRR24 pKa = 11.84ILFTVTIANNQQ35 pKa = 3.18

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

2991

35

388

149.6

17.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.915 ± 0.253

0.936 ± 0.128

6.319 ± 0.522

7.857 ± 0.565

4.112 ± 0.491

4.112 ± 0.43

1.404 ± 0.288

7.355 ± 0.405

10.732 ± 0.656

10.331 ± 0.545

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.307 ± 0.285

6.52 ± 0.582

2.742 ± 0.317

4.146 ± 0.255

4.346 ± 0.386

6.152 ± 0.495

6.118 ± 0.54

4.614 ± 0.39

0.635 ± 0.154

4.346 ± 0.389
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski