Amino acid dipepetide frequency for Phycisphaerae bacterium RAS2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.012AlaAla: 16.012 ± 0.199	1.635AlaCys: 1.635 ± 0.044	6.661AlaAsp: 6.661 ± 0.08	6.288AlaGlu: 6.288 ± 0.102	3.799AlaPhe: 3.799 ± 0.066	9.895AlaGly: 9.895 ± 0.102	2.067AlaHis: 2.067 ± 0.043	5.632AlaIle: 5.632 ± 0.083	3.918AlaLys: 3.918 ± 0.072	10.995AlaLeu: 10.995 ± 0.126	3.378AlaMet: 3.378 ± 0.065	3.013AlaAsn: 3.013 ± 0.064	5.218AlaPro: 5.218 ± 0.077	3.566AlaGln: 3.566 ± 0.065	8.58AlaArg: 8.58 ± 0.111	6.45AlaSer: 6.45 ± 0.081	6.322AlaThr: 6.322 ± 0.082	8.383AlaVal: 8.383 ± 0.088	1.676AlaTrp: 1.676 ± 0.049	2.268AlaTyr: 2.268 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	1.271CysAla: 1.271 ± 0.029	0.287CysCys: 0.287 ± 0.021	0.903CysAsp: 0.903 ± 0.032	0.811CysGlu: 0.811 ± 0.029	0.471CysPhe: 0.471 ± 0.021	1.466CysGly: 1.466 ± 0.048	0.372CysHis: 0.372 ± 0.03	0.582CysIle: 0.582 ± 0.021	0.428CysLys: 0.428 ± 0.02	1.147CysLeu: 1.147 ± 0.035	0.255CysMet: 0.255 ± 0.014	0.439CysAsn: 0.439 ± 0.025	0.83CysPro: 0.83 ± 0.036	0.342CysGln: 0.342 ± 0.018	0.957CysArg: 0.957 ± 0.03	0.724CysSer: 0.724 ± 0.027	0.65CysThr: 0.65 ± 0.029	0.954CysVal: 0.954 ± 0.03	0.172CysTrp: 0.172 ± 0.012	0.303CysTyr: 0.303 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	7.177AspAla: 7.177 ± 0.099	0.798AspCys: 0.798 ± 0.035	3.444AspAsp: 3.444 ± 0.07	3.874AspGlu: 3.874 ± 0.066	2.11AspPhe: 2.11 ± 0.047	5.514AspGly: 5.514 ± 0.111	1.184AspHis: 1.184 ± 0.032	2.485AspIle: 2.485 ± 0.054	1.726AspLys: 1.726 ± 0.043	5.498AspLeu: 5.498 ± 0.08	1.163AspMet: 1.163 ± 0.028	1.398AspAsn: 1.398 ± 0.039	3.588AspPro: 3.588 ± 0.058	1.723AspGln: 1.723 ± 0.039	4.344AspArg: 4.344 ± 0.066	3.117AspSer: 3.117 ± 0.047	2.449AspThr: 2.449 ± 0.058	4.422AspVal: 4.422 ± 0.066	0.923AspTrp: 0.923 ± 0.028	1.456AspTyr: 1.456 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	6.43GluAla: 6.43 ± 0.106	0.621GluCys: 0.621 ± 0.025	2.534GluAsp: 2.534 ± 0.05	2.81GluGlu: 2.81 ± 0.062	2.034GluPhe: 2.034 ± 0.046	3.549GluGly: 3.549 ± 0.06	1.305GluHis: 1.305 ± 0.036	3.078GluIle: 3.078 ± 0.056	2.384GluLys: 2.384 ± 0.064	5.862GluLeu: 5.862 ± 0.083	1.482GluMet: 1.482 ± 0.039	1.485GluAsn: 1.485 ± 0.038	2.964GluPro: 2.964 ± 0.052	2.413GluGln: 2.413 ± 0.061	4.56GluArg: 4.56 ± 0.077	3.691GluSer: 3.691 ± 0.056	2.778GluThr: 2.778 ± 0.047	3.632GluVal: 3.632 ± 0.052	0.766GluTrp: 0.766 ± 0.026	1.412GluTyr: 1.412 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	3.991PheAla: 3.991 ± 0.059	0.532PheCys: 0.532 ± 0.022	2.594PheAsp: 2.594 ± 0.055	2.2PheGlu: 2.2 ± 0.042	1.407PhePhe: 1.407 ± 0.04	3.285PheGly: 3.285 ± 0.06	0.753PheHis: 0.753 ± 0.024	1.623PheIle: 1.623 ± 0.04	1.096PheLys: 1.096 ± 0.036	3.081PheLeu: 3.081 ± 0.062	0.678PheMet: 0.678 ± 0.024	1.272PheAsn: 1.272 ± 0.037	1.527PhePro: 1.527 ± 0.038	1.01PheGln: 1.01 ± 0.032	2.268PheArg: 2.268 ± 0.04	2.126PheSer: 2.126 ± 0.047	2.068PheThr: 2.068 ± 0.052	3.029PheVal: 3.029 ± 0.058	0.508PheTrp: 0.508 ± 0.022	0.895PheTyr: 0.895 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	8.452GlyAla: 8.452 ± 0.097	1.298GlyCys: 1.298 ± 0.048	4.97GlyAsp: 4.97 ± 0.087	4.518GlyGlu: 4.518 ± 0.065	3.066GlyPhe: 3.066 ± 0.058	7.418GlyGly: 7.418 ± 0.127	1.718GlyHis: 1.718 ± 0.039	4.016GlyIle: 4.016 ± 0.071	3.092GlyLys: 3.092 ± 0.065	7.533GlyLeu: 7.533 ± 0.094	2.143GlyMet: 2.143 ± 0.038	2.484GlyAsn: 2.484 ± 0.08	3.753GlyPro: 3.753 ± 0.066	2.825GlyGln: 2.825 ± 0.053	6.362GlyArg: 6.362 ± 0.082	4.766GlySer: 4.766 ± 0.065	4.416GlyThr: 4.416 ± 0.074	5.929GlyVal: 5.929 ± 0.085	1.282GlyTrp: 1.282 ± 0.032	2.083GlyTyr: 2.083 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.376HisAla: 2.376 ± 0.046	0.325HisCys: 0.325 ± 0.019	1.286HisAsp: 1.286 ± 0.033	1.296HisGlu: 1.296 ± 0.034	0.83HisPhe: 0.83 ± 0.024	1.8HisGly: 1.8 ± 0.042	0.615HisHis: 0.615 ± 0.024	0.845HisIle: 0.845 ± 0.023	0.554HisLys: 0.554 ± 0.021	2.03HisLeu: 2.03 ± 0.047	0.421HisMet: 0.421 ± 0.021	0.54HisAsn: 0.54 ± 0.021	1.359HisPro: 1.359 ± 0.038	0.659HisGln: 0.659 ± 0.022	1.82HisArg: 1.82 ± 0.039	1.063HisSer: 1.063 ± 0.033	0.973HisThr: 0.973 ± 0.032	1.651HisVal: 1.651 ± 0.04	0.323HisTrp: 0.323 ± 0.017	0.589HisTyr: 0.589 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.221IleAla: 6.221 ± 0.08	0.661IleCys: 0.661 ± 0.021	3.533IleAsp: 3.533 ± 0.061	3.51IleGlu: 3.51 ± 0.062	1.473IlePhe: 1.473 ± 0.042	4.382IleGly: 4.382 ± 0.069	1.116IleHis: 1.116 ± 0.03	2.121IleIle: 2.121 ± 0.044	1.485IleLys: 1.485 ± 0.037	4.217IleLeu: 4.217 ± 0.069	0.766IleMet: 0.766 ± 0.028	1.535IleAsn: 1.535 ± 0.042	2.508IlePro: 2.508 ± 0.049	1.424IleGln: 1.424 ± 0.039	3.589IleArg: 3.589 ± 0.059	2.599IleSer: 2.599 ± 0.045	2.623IleThr: 2.623 ± 0.048	4.018IleVal: 4.018 ± 0.067	0.554IleTrp: 0.554 ± 0.024	1.005IleTyr: 1.005 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	3.479LysAla: 3.479 ± 0.072	0.374LysCys: 0.374 ± 0.017	1.732LysAsp: 1.732 ± 0.043	1.717LysGlu: 1.717 ± 0.045	1.046LysPhe: 1.046 ± 0.029	2.11LysGly: 2.11 ± 0.044	0.737LysHis: 0.737 ± 0.025	1.638LysIle: 1.638 ± 0.049	1.657LysLys: 1.657 ± 0.054	3.592LysLeu: 3.592 ± 0.068	0.82LysMet: 0.82 ± 0.027	0.989LysAsn: 0.989 ± 0.032	2.17LysPro: 2.17 ± 0.05	1.35LysGln: 1.35 ± 0.04	2.462LysArg: 2.462 ± 0.055	2.171LysSer: 2.171 ± 0.05	1.872LysThr: 1.872 ± 0.045	2.241LysVal: 2.241 ± 0.058	0.455LysTrp: 0.455 ± 0.019	0.811LysTyr: 0.811 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	12.207LeuAla: 12.207 ± 0.146	1.267LeuCys: 1.267 ± 0.031	5.7LeuAsp: 5.7 ± 0.077	4.979LeuGlu: 4.979 ± 0.088	3.304LeuPhe: 3.304 ± 0.064	7.588LeuGly: 7.588 ± 0.089	2.031LeuHis: 2.031 ± 0.042	4.821LeuIle: 4.821 ± 0.073	3.312LeuLys: 3.312 ± 0.06	9.345LeuLeu: 9.345 ± 0.13	2.052LeuMet: 2.052 ± 0.041	2.84LeuAsn: 2.84 ± 0.052	5.126LeuPro: 5.126 ± 0.069	2.712LeuGln: 2.712 ± 0.053	7.436LeuArg: 7.436 ± 0.097	5.386LeuSer: 5.386 ± 0.064	5.499LeuThr: 5.499 ± 0.075	6.915LeuVal: 6.915 ± 0.082	1.161LeuTrp: 1.161 ± 0.038	2.014LeuTyr: 2.014 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	2.728MetAla: 2.728 ± 0.05	0.23MetCys: 0.23 ± 0.013	1.132MetAsp: 1.132 ± 0.027	1.183MetGlu: 1.183 ± 0.033	0.776MetPhe: 0.776 ± 0.024	1.686MetGly: 1.686 ± 0.038	0.513MetHis: 0.513 ± 0.024	1.193MetIle: 1.193 ± 0.033	0.962MetLys: 0.962 ± 0.029	2.46MetLeu: 2.46 ± 0.044	0.623MetMet: 0.623 ± 0.024	0.833MetAsn: 0.833 ± 0.023	1.455MetPro: 1.455 ± 0.038	0.854MetGln: 0.854 ± 0.025	1.585MetArg: 1.585 ± 0.039	1.507MetSer: 1.507 ± 0.035	1.6MetThr: 1.6 ± 0.034	1.527MetVal: 1.527 ± 0.034	0.209MetTrp: 0.209 ± 0.013	0.421MetTyr: 0.421 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.033AsnAla: 3.033 ± 0.06	0.431AsnCys: 0.431 ± 0.022	1.787AsnAsp: 1.787 ± 0.059	1.582AsnGlu: 1.582 ± 0.035	0.981AsnPhe: 0.981 ± 0.029	2.686AsnGly: 2.686 ± 0.078	0.559AsnHis: 0.559 ± 0.018	1.381AsnIle: 1.381 ± 0.034	0.811AsnLys: 0.811 ± 0.027	2.751AsnLeu: 2.751 ± 0.047	0.548AsnMet: 0.548 ± 0.022	1.071AsnAsn: 1.071 ± 0.054	2.086AsnPro: 2.086 ± 0.046	1.027AsnGln: 1.027 ± 0.032	2.066AsnArg: 2.066 ± 0.041	1.598AsnSer: 1.598 ± 0.045	1.496AsnThr: 1.496 ± 0.054	2.357AsnVal: 2.357 ± 0.054	0.482AsnTrp: 0.482 ± 0.019	0.713AsnTyr: 0.713 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	6.532ProAla: 6.532 ± 0.09	0.532ProCys: 0.532 ± 0.025	3.707ProAsp: 3.707 ± 0.065	3.075ProGlu: 3.075 ± 0.049	1.912ProPhe: 1.912 ± 0.045	4.774ProGly: 4.774 ± 0.068	1.063ProHis: 1.063 ± 0.031	2.676ProIle: 2.676 ± 0.041	1.594ProLys: 1.594 ± 0.042	4.335ProLeu: 4.335 ± 0.063	1.488ProMet: 1.488 ± 0.036	1.738ProAsn: 1.738 ± 0.044	3.496ProPro: 3.496 ± 0.065	1.719ProGln: 1.719 ± 0.04	3.637ProArg: 3.637 ± 0.062	3.283ProSer: 3.283 ± 0.06	3.318ProThr: 3.318 ± 0.056	3.978ProVal: 3.978 ± 0.064	0.715ProTrp: 0.715 ± 0.024	1.06ProTyr: 1.06 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	3.585GlnAla: 3.585 ± 0.07	0.411GlnCys: 0.411 ± 0.018	1.379GlnAsp: 1.379 ± 0.034	1.512GlnGlu: 1.512 ± 0.041	1.289GlnPhe: 1.289 ± 0.03	2.008GlnGly: 2.008 ± 0.043	0.735GlnHis: 0.735 ± 0.025	1.799GlnIle: 1.799 ± 0.038	1.163GlnLys: 1.163 ± 0.036	3.221GlnLeu: 3.221 ± 0.055	0.842GlnMet: 0.842 ± 0.026	0.899GlnAsn: 0.899 ± 0.029	2.16GlnPro: 2.16 ± 0.042	1.383GlnGln: 1.383 ± 0.044	2.847GlnArg: 2.847 ± 0.044	2.114GlnSer: 2.114 ± 0.04	1.836GlnThr: 1.836 ± 0.038	2.409GlnVal: 2.409 ± 0.049	0.551GlnTrp: 0.551 ± 0.019	0.786GlnTyr: 0.786 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	7.889ArgAla: 7.889 ± 0.096	0.879ArgCys: 0.879 ± 0.03	4.366ArgAsp: 4.366 ± 0.07	4.704ArgGlu: 4.704 ± 0.078	2.97ArgPhe: 2.97 ± 0.049	5.259ArgGly: 5.259 ± 0.077	1.761ArgHis: 1.761 ± 0.041	4.206ArgIle: 4.206 ± 0.061	2.459ArgLys: 2.459 ± 0.056	7.597ArgLeu: 7.597 ± 0.115	1.921ArgMet: 1.921 ± 0.038	1.963ArgAsn: 1.963 ± 0.037	3.875ArgPro: 3.875 ± 0.06	2.573ArgGln: 2.573 ± 0.057	6.923ArgArg: 6.923 ± 0.101	4.07ArgSer: 4.07 ± 0.057	3.698ArgThr: 3.698 ± 0.058	5.634ArgVal: 5.634 ± 0.073	1.201ArgTrp: 1.201 ± 0.033	1.842ArgTyr: 1.842 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.549SerAla: 6.549 ± 0.087	0.73SerCys: 0.73 ± 0.029	3.195SerAsp: 3.195 ± 0.054	2.824SerGlu: 2.824 ± 0.049	2.085SerPhe: 2.085 ± 0.044	5.321SerGly: 5.321 ± 0.08	1.255SerHis: 1.255 ± 0.035	2.934SerIle: 2.934 ± 0.052	1.707SerLys: 1.707 ± 0.044	5.36SerLeu: 5.36 ± 0.066	1.3SerMet: 1.3 ± 0.03	1.896SerAsn: 1.896 ± 0.043	3.566SerPro: 3.566 ± 0.056	1.973SerGln: 1.973 ± 0.041	4.351SerArg: 4.351 ± 0.067	3.315SerSer: 3.315 ± 0.065	3.122SerThr: 3.122 ± 0.052	4.0SerVal: 4.0 ± 0.063	0.743SerTrp: 0.743 ± 0.025	1.215SerTyr: 1.215 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	6.102ThrAla: 6.102 ± 0.076	0.709ThrCys: 0.709 ± 0.028	3.114ThrAsp: 3.114 ± 0.057	2.408ThrGlu: 2.408 ± 0.049	1.986ThrPhe: 1.986 ± 0.044	5.074ThrGly: 5.074 ± 0.07	1.111ThrHis: 1.111 ± 0.029	2.903ThrIle: 2.903 ± 0.056	1.522ThrLys: 1.522 ± 0.045	5.427ThrLeu: 5.427 ± 0.073	1.122ThrMet: 1.122 ± 0.028	1.679ThrAsn: 1.679 ± 0.05	3.491ThrPro: 3.491 ± 0.057	1.688ThrGln: 1.688 ± 0.04	3.426ThrArg: 3.426 ± 0.051	2.971ThrSer: 2.971 ± 0.055	3.256ThrThr: 3.256 ± 0.067	4.193ThrVal: 4.193 ± 0.067	0.822ThrTrp: 0.822 ± 0.026	1.293ThrTyr: 1.293 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	8.274ValAla: 8.274 ± 0.08	1.111ValCys: 1.111 ± 0.03	4.161ValAsp: 4.161 ± 0.057	4.405ValGlu: 4.405 ± 0.062	2.803ValPhe: 2.803 ± 0.056	5.585ValGly: 5.585 ± 0.078	1.537ValHis: 1.537 ± 0.042	3.768ValIle: 3.768 ± 0.063	2.335ValLys: 2.335 ± 0.052	7.303ValLeu: 7.303 ± 0.082	1.674ValMet: 1.674 ± 0.038	2.245ValAsn: 2.245 ± 0.05	3.648ValPro: 3.648 ± 0.06	2.321ValGln: 2.321 ± 0.04	5.508ValArg: 5.508 ± 0.074	4.283ValSer: 4.283 ± 0.055	4.389ValThr: 4.389 ± 0.07	5.894ValVal: 5.894 ± 0.084	0.928ValTrp: 0.928 ± 0.031	1.655ValTyr: 1.655 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.22TrpAla: 1.22 ± 0.037	0.194TrpCys: 0.194 ± 0.013	0.705TrpAsp: 0.705 ± 0.025	0.652TrpGlu: 0.652 ± 0.026	0.525TrpPhe: 0.525 ± 0.021	0.883TrpGly: 0.883 ± 0.028	0.363TrpHis: 0.363 ± 0.017	0.749TrpIle: 0.749 ± 0.026	0.557TrpLys: 0.557 ± 0.023	1.619TrpLeu: 1.619 ± 0.045	0.388TrpMet: 0.388 ± 0.019	0.446TrpAsn: 0.446 ± 0.022	0.755TrpPro: 0.755 ± 0.023	0.572TrpGln: 0.572 ± 0.019	1.19TrpArg: 1.19 ± 0.035	0.918TrpSer: 0.918 ± 0.031	0.83TrpThr: 0.83 ± 0.029	0.91TrpVal: 0.91 ± 0.028	0.269TrpTrp: 0.269 ± 0.017	0.349TrpTyr: 0.349 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.228TyrAla: 2.228 ± 0.046	0.34TyrCys: 0.34 ± 0.015	1.491TyrAsp: 1.491 ± 0.039	1.441TyrGlu: 1.441 ± 0.031	1.075TyrPhe: 1.075 ± 0.032	1.842TyrGly: 1.842 ± 0.05	0.559TyrHis: 0.559 ± 0.021	0.892TyrIle: 0.892 ± 0.028	0.664TyrLys: 0.664 ± 0.02	2.321TyrLeu: 2.321 ± 0.044	0.41TyrMet: 0.41 ± 0.016	0.681TyrAsn: 0.681 ± 0.026	1.059TyrPro: 1.059 ± 0.032	0.794TyrGln: 0.794 ± 0.028	1.883TyrArg: 1.883 ± 0.038	1.286TyrSer: 1.286 ± 0.038	1.101TyrThr: 1.101 ± 0.034	1.751TyrVal: 1.751 ± 0.038	0.352TyrTrp: 0.352 ± 0.017	0.726TyrTyr: 0.726 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3554 proteins (1265709 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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