Pontiella sulfatireligans
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5575 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6C2UL90|A0A6C2UL90_9BACT Uncharacterized protein OS=Pontiella sulfatireligans OX=2750658 GN=SCARR_03069 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 9.86 MRR4 pKa = 11.84 GITLFAALLGMAIGTQAALPEE25 pKa = 4.61 IGDD28 pKa = 4.36 TIWLKK33 pKa = 11.17 GGTDD37 pKa = 3.15 NSYY40 pKa = 11.26 VYY42 pKa = 10.89 ASGSALVAKK51 pKa = 10.75 AEE53 pKa = 4.12 TDD55 pKa = 3.45 VANALNFVVHH65 pKa = 6.32 SADD68 pKa = 3.55 GSDD71 pKa = 3.44 SNFIFQVADD80 pKa = 3.38 TTSYY84 pKa = 11.24 VIVVTNNDD92 pKa = 4.37 DD93 pKa = 3.56 ILTIDD98 pKa = 3.9 ATALTNNPLTHH109 pKa = 6.23 FTMTEE114 pKa = 3.75 ITEE117 pKa = 3.94 GTYY120 pKa = 10.73 AGTYY124 pKa = 8.86 EE125 pKa = 4.53 LSCLAHH131 pKa = 7.11 ADD133 pKa = 3.81 PVVHH137 pKa = 6.48 EE138 pKa = 4.99 RR139 pKa = 11.84 GNTHH143 pKa = 5.5 TLKK146 pKa = 10.8 AHH148 pKa = 5.12 STSGGSIRR156 pKa = 11.84 SAWTYY161 pKa = 10.51 GVVGAALEE169 pKa = 4.4 PPTGLGANTSDD180 pKa = 3.62 SQVVLDD186 pKa = 4.11 WDD188 pKa = 3.93 NDD190 pKa = 3.56 VSGLLDD196 pKa = 3.62 TYY198 pKa = 9.92 TVYY201 pKa = 10.98 RR202 pKa = 11.84 STNTPVTISDD212 pKa = 3.95 TLLGSPSNSQFTDD225 pKa = 3.41 TNVVNGTTYY234 pKa = 10.8 YY235 pKa = 10.52 YY236 pKa = 11.19 AVTATDD242 pKa = 4.35 GSTEE246 pKa = 3.93 TDD248 pKa = 3.11 LSNEE252 pKa = 3.96 VSATPPTGTAQPKK265 pKa = 9.13 RR266 pKa = 11.84 LKK268 pKa = 10.89 ALGVPAAVLLDD279 pKa = 3.59 WADD282 pKa = 3.63 DD283 pKa = 4.05 TTGHH287 pKa = 6.94 LDD289 pKa = 3.25 YY290 pKa = 10.29 YY291 pKa = 9.17 TVYY294 pKa = 10.76 RR295 pKa = 11.84 STTTPVTTGSPVLTNVTASAFTDD318 pKa = 3.99 FDD320 pKa = 4.11 VVAGTTNYY328 pKa = 10.46 YY329 pKa = 11.0 AVTAVGTNGVPPEE342 pKa = 4.22 SALSDD347 pKa = 3.67 EE348 pKa = 4.67 VSAIPFAAVTEE359 pKa = 4.56 TVLFQHH365 pKa = 6.95 IDD367 pKa = 3.19 ATVAASVQTDD377 pKa = 3.47 GVTNGMGIVTDD388 pKa = 4.02 VVDD391 pKa = 4.11 QSTFGNDD398 pKa = 2.95 ATDD401 pKa = 3.46 VADD404 pKa = 5.2 GDD406 pKa = 4.32 DD407 pKa = 4.8 LGPVLWPSTDD417 pKa = 3.13 LFGSGRR423 pKa = 11.84 AGFDD427 pKa = 2.72 MGTDD431 pKa = 3.17 MRR433 pKa = 11.84 MLNLFTNGTDD443 pKa = 3.24 ALLDD447 pKa = 3.79 FTGDD451 pKa = 3.28 ASGSDD456 pKa = 3.64 GFAMLVAFKK465 pKa = 10.13 TRR467 pKa = 11.84 TMTEE471 pKa = 3.73 GVANIFLNNGPISMEE486 pKa = 3.7 YY487 pKa = 10.62 DD488 pKa = 3.44 GDD490 pKa = 4.16 DD491 pKa = 3.67 GEE493 pKa = 4.68 MTVEE497 pKa = 4.54 LGSATLTQSGAGVEE511 pKa = 4.21 AGDD514 pKa = 4.0 TIVYY518 pKa = 9.66 ALNYY522 pKa = 9.29 NALTGEE528 pKa = 4.26 TTFWDD533 pKa = 3.6 SKK535 pKa = 10.71 NNEE538 pKa = 4.3 EE539 pKa = 4.54 VSVTAEE545 pKa = 3.64 AHH547 pKa = 6.43 GDD549 pKa = 3.65 FSGDD553 pKa = 3.47 PLQLGGSGRR562 pKa = 11.84 DD563 pKa = 3.27 SRR565 pKa = 11.84 IFDD568 pKa = 3.5 GLMGEE573 pKa = 4.38 VKK575 pKa = 10.22 IFSTKK580 pKa = 10.37 LPADD584 pKa = 3.27 QFEE587 pKa = 4.43 AQRR590 pKa = 11.84 DD591 pKa = 3.74 ALRR594 pKa = 11.84 LKK596 pKa = 9.46 WVSADD601 pKa = 3.16 ATGFDD606 pKa = 3.72 VWVSSWGVNLGSATNDD622 pKa = 3.01 WDD624 pKa = 3.87 NDD626 pKa = 4.41 GINNLAEE633 pKa = 3.98 YY634 pKa = 10.53 AFAGNPTNGTDD645 pKa = 3.45 DD646 pKa = 3.96 TYY648 pKa = 11.58 TDD650 pKa = 3.97 LVTDD654 pKa = 4.26 GGLVYY659 pKa = 10.49 IHH661 pKa = 6.67 PQPKK665 pKa = 9.78 NDD667 pKa = 3.6 ADD669 pKa = 3.88 LNYY672 pKa = 10.99 AVLTMDD678 pKa = 4.51 NLPYY682 pKa = 9.81 DD683 pKa = 3.26 TWTDD687 pKa = 3.06 EE688 pKa = 4.46 GYY690 pKa = 8.14 TAVIGTYY697 pKa = 10.04 VPAEE701 pKa = 3.55 GDD703 pKa = 3.5 YY704 pKa = 11.17 NYY706 pKa = 10.5 VSNSVPTTDD715 pKa = 2.67 AVKK718 pKa = 10.31 FIRR721 pKa = 11.84 ASAEE725 pKa = 3.91 YY726 pKa = 10.63
Molecular weight: 76.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.673
IPC2_protein 3.745
IPC_protein 3.795
Toseland 3.567
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.948
Wikipedia 3.757
Rodwell 3.617
Grimsley 3.465
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.202
Thurlkill 3.617
EMBOSS 3.757
Sillero 3.923
Patrickios 1.252
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.814
Protein with the highest isoelectric point:
>tr|A0A6C2US34|A0A6C2US34_9BACT Diaminobutyrate--2-oxoglutarate transaminase OS=Pontiella sulfatireligans OX=2750658 GN=ectB PE=3 SV=1
MM1 pKa = 7.73 KK2 pKa = 10.12 KK3 pKa = 9.79 RR4 pKa = 11.84 KK5 pKa = 9.32 KK6 pKa = 9.06 KK7 pKa = 9.89 HH8 pKa = 5.77 RR9 pKa = 11.84 PRR11 pKa = 11.84 KK12 pKa = 9.55 HH13 pKa = 5.39 NQQHH17 pKa = 5.14 TGKK20 pKa = 9.41 VAYY23 pKa = 8.78 MNSGRR28 pKa = 11.84 IKK30 pKa = 10.08 STLQTMRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 MSS42 pKa = 2.94
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.365
IPC2_protein 11.008
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.076
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 8.991
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5575
0
5575
2209931
29
3911
396.4
43.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.858 ± 0.031
1.218 ± 0.012
6.067 ± 0.025
6.303 ± 0.03
4.17 ± 0.015
8.237 ± 0.031
2.174 ± 0.017
5.472 ± 0.022
5.227 ± 0.034
8.987 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.596 ± 0.013
4.2 ± 0.024
4.694 ± 0.022
3.279 ± 0.017
4.949 ± 0.026
6.147 ± 0.027
5.578 ± 0.03
6.801 ± 0.028
1.679 ± 0.015
3.366 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here