Sphingomonas sp. CL5.1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4226 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N0WWD1|A0A6N0WWD1_9SPHN Cytochrome P450 OS=Sphingomonas sp. CL5.1 OX=2653203 GN=F9288_00855 PE=3 SV=1
MM1 pKa = 7.62SNTTSVGINLSPVNYY16 pKa = 9.43WSTEE20 pKa = 3.81YY21 pKa = 10.61PFLDD25 pKa = 3.21RR26 pKa = 11.84MKK28 pKa = 10.43TAGTWGVQGTSVSSLIVDD46 pKa = 3.38QNGYY50 pKa = 6.9PTTIPTGATSVSTIIALDD68 pKa = 3.77PASAGTDD75 pKa = 3.1NTYY78 pKa = 11.19VLTYY82 pKa = 9.98TGTATFRR89 pKa = 11.84IPGATIVSSKK99 pKa = 10.06PGEE102 pKa = 3.78ITFTVTGANPMQTVMITSIAASNPLTAMHH131 pKa = 6.67VVRR134 pKa = 11.84QDD136 pKa = 3.08QMALFNQGEE145 pKa = 4.54LFNPAFVKK153 pKa = 10.42QVSQFGTLRR162 pKa = 11.84YY163 pKa = 9.06MDD165 pKa = 3.95WEE167 pKa = 4.19QTNITTATSWSQRR180 pKa = 11.84TTPDD184 pKa = 3.15TLSWASTGSTTGVPIEE200 pKa = 4.6AMVALANEE208 pKa = 4.48SRR210 pKa = 11.84TNMWLNIPTQANDD223 pKa = 3.44DD224 pKa = 4.19YY225 pKa = 10.74VRR227 pKa = 11.84QMLTYY232 pKa = 10.6VRR234 pKa = 11.84DD235 pKa = 3.85NLDD238 pKa = 3.28PSLKK242 pKa = 10.86VNVEE246 pKa = 4.06YY247 pKa = 11.17SNEE250 pKa = 3.45MWNWGFQQSRR260 pKa = 11.84YY261 pKa = 10.09ANQLATQLWGTNVTRR276 pKa = 11.84GVQQYY281 pKa = 9.6YY282 pKa = 10.54GYY284 pKa = 10.77RR285 pKa = 11.84SAQIAAIANDD295 pKa = 3.56VFGDD299 pKa = 4.58GAATRR304 pKa = 11.84LDD306 pKa = 3.73NVLATQTANLGLEE319 pKa = 4.04KK320 pKa = 11.0YY321 pKa = 10.08IFEE324 pKa = 4.59GVAKK328 pKa = 10.5AGLGDD333 pKa = 3.71VSSLFQSYY341 pKa = 10.53AVTTYY346 pKa = 10.45FGSEE350 pKa = 3.64LSGQNSADD358 pKa = 3.87RR359 pKa = 11.84ATILGWAKK367 pKa = 10.49GGSAGLDD374 pKa = 3.0AAFNEE379 pKa = 4.5IANGGTLTGWGSLPSIIAQWAYY401 pKa = 9.78QATVAKK407 pKa = 10.27KK408 pKa = 10.18YY409 pKa = 10.87GLDD412 pKa = 3.26LVAYY416 pKa = 9.0EE417 pKa = 4.54GGIDD421 pKa = 3.77LSAAGFSAADD431 pKa = 3.42QPIVMDD437 pKa = 3.85FFSRR441 pKa = 11.84LEE443 pKa = 4.04ADD445 pKa = 3.22PRR447 pKa = 11.84MATIYY452 pKa = 9.78TQMIDD457 pKa = 3.19SFSAAGGTLLNAYY470 pKa = 8.71TDD472 pKa = 3.88AQVDD476 pKa = 4.2RR477 pKa = 11.84PGGLYY482 pKa = 8.52GTLKK486 pKa = 10.51SIYY489 pKa = 10.19DD490 pKa = 3.72SASPAWSAPVNGQAEE505 pKa = 4.3ASKK508 pKa = 11.14ASGPVPANNVSGLPAANQPTPVAQNITTAASSYY541 pKa = 8.04TLEE544 pKa = 4.53SGAMTLSYY552 pKa = 10.01IGQSGFVGTGNDD564 pKa = 3.79LDD566 pKa = 4.11NVITGGNSGNKK577 pKa = 10.0LYY579 pKa = 11.06GGAGNDD585 pKa = 3.77TLIGGAGNDD594 pKa = 3.79YY595 pKa = 11.17LDD597 pKa = 4.62GGTGADD603 pKa = 3.73TMTGGAGNDD612 pKa = 3.12IYY614 pKa = 10.92IVDD617 pKa = 3.85NPGDD621 pKa = 3.97VVVEE625 pKa = 4.13AANGGTDD632 pKa = 3.54EE633 pKa = 4.57VRR635 pKa = 11.84TSLDD639 pKa = 3.4SYY641 pKa = 10.79TLGANIEE648 pKa = 4.16NLTYY652 pKa = 9.9TGSGAFTGTGNDD664 pKa = 3.55LANVITGGDD673 pKa = 3.99GGNKK677 pKa = 10.1LYY679 pKa = 10.73GYY681 pKa = 10.05DD682 pKa = 4.74GNDD685 pKa = 3.4TLIGGAGNDD694 pKa = 3.79YY695 pKa = 11.17LDD697 pKa = 4.56GGTGADD703 pKa = 3.44RR704 pKa = 11.84MVGGAGNDD712 pKa = 3.53TYY714 pKa = 11.17IVDD717 pKa = 3.73NPGDD721 pKa = 3.85VVVEE725 pKa = 4.01LPGGGIDD732 pKa = 4.25EE733 pKa = 4.59VRR735 pKa = 11.84TSLGSYY741 pKa = 10.57ALGANLEE748 pKa = 4.21NLTATGSSTFTGIGNAADD766 pKa = 3.78NVITGSTLGANKK778 pKa = 10.49LYY780 pKa = 10.69GYY782 pKa = 10.12DD783 pKa = 4.45GNDD786 pKa = 3.4TLIGGAGNDD795 pKa = 3.79YY796 pKa = 11.17LDD798 pKa = 4.36GGTGADD804 pKa = 3.82YY805 pKa = 9.51MAGGDD810 pKa = 3.73GDD812 pKa = 3.84DD813 pKa = 4.28TYY815 pKa = 12.05VVDD818 pKa = 3.83NPGDD822 pKa = 3.77VVVEE826 pKa = 4.01LPGGGTDD833 pKa = 3.51TVVSSISYY841 pKa = 8.76TLGANLEE848 pKa = 3.95NLRR851 pKa = 11.84LGGSGNINGAGNDD864 pKa = 3.44AANIITGNAGNNILSGGGGDD884 pKa = 5.44DD885 pKa = 3.61VLQGGDD891 pKa = 3.55GDD893 pKa = 5.12DD894 pKa = 4.04YY895 pKa = 11.89LDD897 pKa = 4.62GGDD900 pKa = 4.74GNDD903 pKa = 3.96SLSGGNGNDD912 pKa = 3.2ILIGGAGNDD921 pKa = 3.76YY922 pKa = 11.12LSGGNGNDD930 pKa = 3.5VLIGGPGKK938 pKa = 10.4DD939 pKa = 3.55SLVGGAGADD948 pKa = 2.92RR949 pKa = 11.84FVFAPGDD956 pKa = 3.67LGNTITNTDD965 pKa = 3.76TILDD969 pKa = 4.18FSHH972 pKa = 7.49AEE974 pKa = 3.66GDD976 pKa = 4.82KK977 pKa = 10.6IDD979 pKa = 5.17LSQFDD984 pKa = 4.93ANTNTIAKK992 pKa = 9.96DD993 pKa = 3.16AFTFIANKK1001 pKa = 9.55PFSHH1005 pKa = 6.56TAGEE1009 pKa = 4.22VRR1011 pKa = 11.84VDD1013 pKa = 4.26SIGGYY1018 pKa = 7.77WNVMGDD1024 pKa = 3.64TNGDD1028 pKa = 3.37GVADD1032 pKa = 4.49FALNVKK1038 pKa = 10.46ASGPLVQSDD1047 pKa = 4.87FILGG1051 pKa = 3.53

Molecular weight: 107.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N0X6D9|A0A6N0X6D9_9SPHN DUF1778 domain-containing protein OS=Sphingomonas sp. CL5.1 OX=2653203 GN=F9288_01675 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4226

0

4226

1378064

29

1920

326.1

35.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.036 ± 0.065

0.759 ± 0.011

5.929 ± 0.029

5.198 ± 0.037

3.495 ± 0.021

9.103 ± 0.04

2.015 ± 0.017

4.989 ± 0.022

2.654 ± 0.027

9.719 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.018

2.461 ± 0.028

5.446 ± 0.025

2.827 ± 0.022

7.791 ± 0.036

4.913 ± 0.033

5.283 ± 0.029

7.303 ± 0.032

1.444 ± 0.015

2.28 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski