Amino acid dipepetide frequency for Sphingomonas sp. CL5.1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.66AlaAla: 21.66 ± 0.209
1.043AlaCys: 1.043 ± 0.033
7.953AlaAsp: 7.953 ± 0.088
7.665AlaGlu: 7.665 ± 0.083
4.501AlaPhe: 4.501 ± 0.06
12.796AlaGly: 12.796 ± 0.111
2.584AlaHis: 2.584 ± 0.046
7.377AlaIle: 7.377 ± 0.076
3.718AlaLys: 3.718 ± 0.061
14.711AlaLeu: 14.711 ± 0.149
3.894AlaMet: 3.894 ± 0.055
3.207AlaAsn: 3.207 ± 0.049
6.937AlaPro: 6.937 ± 0.091
4.076AlaGln: 4.076 ± 0.058
11.287AlaArg: 11.287 ± 0.111
6.392AlaSer: 6.392 ± 0.067
7.211AlaThr: 7.211 ± 0.078
8.927AlaVal: 8.927 ± 0.097
1.757AlaTrp: 1.757 ± 0.041
2.664AlaTyr: 2.664 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
1.046CysAla: 1.046 ± 0.028
0.097CysCys: 0.097 ± 0.009
0.512CysAsp: 0.512 ± 0.019
0.375CysGlu: 0.375 ± 0.017
0.301CysPhe: 0.301 ± 0.014
0.871CysGly: 0.871 ± 0.025
0.199CysHis: 0.199 ± 0.012
0.34CysIle: 0.34 ± 0.015
0.142CysLys: 0.142 ± 0.01
0.633CysLeu: 0.633 ± 0.021
0.138CysMet: 0.138 ± 0.009
0.164CysAsn: 0.164 ± 0.011
0.407CysPro: 0.407 ± 0.019
0.181CysGln: 0.181 ± 0.011
0.593CysArg: 0.593 ± 0.022
0.39CysSer: 0.39 ± 0.016
0.355CysThr: 0.355 ± 0.015
0.561CysVal: 0.561 ± 0.021
0.108CysTrp: 0.108 ± 0.009
0.176CysTyr: 0.176 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
8.33AspAla: 8.33 ± 0.078
0.447AspCys: 0.447 ± 0.021
3.287AspAsp: 3.287 ± 0.06
3.285AspGlu: 3.285 ± 0.057
2.123AspPhe: 2.123 ± 0.041
5.635AspGly: 5.635 ± 0.071
1.321AspHis: 1.321 ± 0.036
2.773AspIle: 2.773 ± 0.048
1.496AspLys: 1.496 ± 0.037
5.585AspLeu: 5.585 ± 0.07
1.348AspMet: 1.348 ± 0.034
1.295AspAsn: 1.295 ± 0.032
3.765AspPro: 3.765 ± 0.058
1.696AspGln: 1.696 ± 0.034
5.168AspArg: 5.168 ± 0.065
2.157AspSer: 2.157 ± 0.04
2.685AspThr: 2.685 ± 0.046
4.083AspVal: 4.083 ± 0.057
1.112AspTrp: 1.112 ± 0.03
1.703AspTyr: 1.703 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
7.578GluAla: 7.578 ± 0.088
0.298GluCys: 0.298 ± 0.013
2.429GluAsp: 2.429 ± 0.043
2.763GluGlu: 2.763 ± 0.055
1.403GluPhe: 1.403 ± 0.035
4.283GluGly: 4.283 ± 0.065
1.166GluHis: 1.166 ± 0.03
2.842GluIle: 2.842 ± 0.046
1.681GluLys: 1.681 ± 0.032
4.836GluLeu: 4.836 ± 0.076
1.39GluMet: 1.39 ± 0.032
1.213GluAsn: 1.213 ± 0.034
2.452GluPro: 2.452 ± 0.042
1.893GluGln: 1.893 ± 0.039
5.321GluArg: 5.321 ± 0.075
2.139GluSer: 2.139 ± 0.04
3.011GluThr: 3.011 ± 0.056
3.612GluVal: 3.612 ± 0.048
0.734GluTrp: 0.734 ± 0.023
0.932GluTyr: 0.932 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
4.918PheAla: 4.918 ± 0.06
0.347PheCys: 0.347 ± 0.016
2.639PheAsp: 2.639 ± 0.048
1.877PheGlu: 1.877 ± 0.039
1.209PhePhe: 1.209 ± 0.036
3.761PheGly: 3.761 ± 0.054
0.75PheHis: 0.75 ± 0.023
1.314PheIle: 1.314 ± 0.035
0.71PheLys: 0.71 ± 0.024
2.969PheLeu: 2.969 ± 0.053
0.7PheMet: 0.7 ± 0.023
1.059PheAsn: 1.059 ± 0.033
1.583PhePro: 1.583 ± 0.033
0.839PheGln: 0.839 ± 0.03
2.323PheArg: 2.323 ± 0.041
1.918PheSer: 1.918 ± 0.039
2.005PheThr: 2.005 ± 0.039
2.652PheVal: 2.652 ± 0.045
0.504PheTrp: 0.504 ± 0.018
0.872PheTyr: 0.872 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
11.089GlyAla: 11.089 ± 0.109
0.811GlyCys: 0.811 ± 0.028
5.111GlyAsp: 5.111 ± 0.061
4.913GlyGlu: 4.913 ± 0.064
3.678GlyPhe: 3.678 ± 0.058
8.667GlyGly: 8.667 ± 0.111
1.916GlyHis: 1.916 ± 0.043
4.518GlyIle: 4.518 ± 0.062
3.13GlyLys: 3.13 ± 0.055
8.511GlyLeu: 8.511 ± 0.09
2.48GlyMet: 2.48 ± 0.05
2.32GlyAsn: 2.32 ± 0.045
3.863GlyPro: 3.863 ± 0.048
2.829GlyGln: 2.829 ± 0.052
6.945GlyArg: 6.945 ± 0.077
4.586GlySer: 4.586 ± 0.061
4.859GlyThr: 4.859 ± 0.069
6.58GlyVal: 6.58 ± 0.073
1.703GlyTrp: 1.703 ± 0.04
2.521GlyTyr: 2.521 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.53HisAla: 2.53 ± 0.048
0.206HisCys: 0.206 ± 0.013
1.307HisAsp: 1.307 ± 0.03
0.994HisGlu: 0.994 ± 0.029
0.793HisPhe: 0.793 ± 0.024
2.096HisGly: 2.096 ± 0.042
0.567HisHis: 0.567 ± 0.022
0.896HisIle: 0.896 ± 0.027
0.385HisLys: 0.385 ± 0.018
1.899HisLeu: 1.899 ± 0.043
0.454HisMet: 0.454 ± 0.019
0.452HisAsn: 0.452 ± 0.018
1.356HisPro: 1.356 ± 0.03
0.517HisGln: 0.517 ± 0.019
1.606HisArg: 1.606 ± 0.04
0.844HisSer: 0.844 ± 0.027
0.696HisThr: 0.696 ± 0.022
1.576HisVal: 1.576 ± 0.031
0.377HisTrp: 0.377 ± 0.017
0.601HisTyr: 0.601 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
8.56IleAla: 8.56 ± 0.089
0.432IleCys: 0.432 ± 0.02
4.044IleAsp: 4.044 ± 0.059
3.352IleGlu: 3.352 ± 0.054
1.443IlePhe: 1.443 ± 0.033
5.321IleGly: 5.321 ± 0.061
0.912IleHis: 0.912 ± 0.019
1.776IleIle: 1.776 ± 0.042
0.993IleLys: 0.993 ± 0.028
3.71IleLeu: 3.71 ± 0.052
0.81IleMet: 0.81 ± 0.025
1.223IleAsn: 1.223 ± 0.032
2.163IlePro: 2.163 ± 0.043
1.102IleGln: 1.102 ± 0.024
3.309IleArg: 3.309 ± 0.048
2.236IleSer: 2.236 ± 0.042
2.464IleThr: 2.464 ± 0.049
4.418IleVal: 4.418 ± 0.07
0.572IleTrp: 0.572 ± 0.024
1.048IleTyr: 1.048 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
3.681LysAla: 3.681 ± 0.07
0.131LysCys: 0.131 ± 0.01
1.332LysAsp: 1.332 ± 0.032
1.115LysGlu: 1.115 ± 0.034
0.755LysPhe: 0.755 ± 0.025
2.305LysGly: 2.305 ± 0.045
0.47LysHis: 0.47 ± 0.02
1.257LysIle: 1.257 ± 0.03
0.822LysLys: 0.822 ± 0.027
2.845LysLeu: 2.845 ± 0.054
0.675LysMet: 0.675 ± 0.022
0.614LysAsn: 0.614 ± 0.021
1.821LysPro: 1.821 ± 0.044
0.767LysGln: 0.767 ± 0.025
2.117LysArg: 2.117 ± 0.043
1.345LysSer: 1.345 ± 0.035
1.412LysThr: 1.412 ± 0.038
2.149LysVal: 2.149 ± 0.042
0.364LysTrp: 0.364 ± 0.017
0.565LysTyr: 0.565 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
14.977LeuAla: 14.977 ± 0.144
0.71LeuCys: 0.71 ± 0.022
6.236LeuAsp: 6.236 ± 0.079
4.49LeuGlu: 4.49 ± 0.065
3.376LeuPhe: 3.376 ± 0.058
8.428LeuGly: 8.428 ± 0.08
1.808LeuHis: 1.808 ± 0.037
4.66LeuIle: 4.66 ± 0.057
2.752LeuLys: 2.752 ± 0.05
9.148LeuLeu: 9.148 ± 0.102
2.027LeuMet: 2.027 ± 0.037
2.339LeuAsn: 2.339 ± 0.046
5.688LeuPro: 5.688 ± 0.065
2.136LeuGln: 2.136 ± 0.037
6.993LeuArg: 6.993 ± 0.082
5.522LeuSer: 5.522 ± 0.065
5.424LeuThr: 5.424 ± 0.066
7.297LeuVal: 7.297 ± 0.082
1.16LeuTrp: 1.16 ± 0.028
2.017LeuTyr: 2.017 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
3.151MetAla: 3.151 ± 0.051
0.155MetCys: 0.155 ± 0.01
1.003MetAsp: 1.003 ± 0.026
0.985MetGlu: 0.985 ± 0.026
0.74MetPhe: 0.74 ± 0.022
1.792MetGly: 1.792 ± 0.041
0.415MetHis: 0.415 ± 0.019
1.386MetIle: 1.386 ± 0.031
0.861MetLys: 0.861 ± 0.026
2.51MetLeu: 2.51 ± 0.045
0.608MetMet: 0.608 ± 0.025
0.639MetAsn: 0.639 ± 0.02
1.442MetPro: 1.442 ± 0.036
0.626MetGln: 0.626 ± 0.021
1.944MetArg: 1.944 ± 0.037
1.371MetSer: 1.371 ± 0.031
1.828MetThr: 1.828 ± 0.036
1.592MetVal: 1.592 ± 0.031
0.238MetTrp: 0.238 ± 0.013
0.266MetTyr: 0.266 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.228AsnAla: 3.228 ± 0.045
0.208AsnCys: 0.208 ± 0.013
1.323AsnAsp: 1.323 ± 0.034
1.037AsnGlu: 1.037 ± 0.028
0.93AsnPhe: 0.93 ± 0.031
2.394AsnGly: 2.394 ± 0.044
0.475AsnHis: 0.475 ± 0.018
1.303AsnIle: 1.303 ± 0.033
0.514AsnLys: 0.514 ± 0.022
2.432AsnLeu: 2.432 ± 0.048
0.517AsnMet: 0.517 ± 0.019
0.691AsnAsn: 0.691 ± 0.025
1.736AsnPro: 1.736 ± 0.037
0.723AsnGln: 0.723 ± 0.025
1.767AsnArg: 1.767 ± 0.038
1.126AsnSer: 1.126 ± 0.037
1.217AsnThr: 1.217 ± 0.044
1.856AsnVal: 1.856 ± 0.037
0.435AsnTrp: 0.435 ± 0.02
0.702AsnTyr: 0.702 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
7.825ProAla: 7.825 ± 0.087
0.324ProCys: 0.324 ± 0.014
3.839ProAsp: 3.839 ± 0.057
3.426ProGlu: 3.426 ± 0.051
1.908ProPhe: 1.908 ± 0.037
5.129ProGly: 5.129 ± 0.063
1.03ProHis: 1.03 ± 0.029
2.516ProIle: 2.516 ± 0.038
1.377ProLys: 1.377 ± 0.033
5.1ProLeu: 5.1 ± 0.061
1.189ProMet: 1.189 ± 0.028
1.186ProAsn: 1.186 ± 0.034
3.08ProPro: 3.08 ± 0.066
1.519ProGln: 1.519 ± 0.033
3.496ProArg: 3.496 ± 0.058
2.578ProSer: 2.578 ± 0.051
2.587ProThr: 2.587 ± 0.041
4.439ProVal: 4.439 ± 0.056
0.745ProTrp: 0.745 ± 0.024
1.165ProTyr: 1.165 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
3.736GlnAla: 3.736 ± 0.056
0.204GlnCys: 0.204 ± 0.015
1.238GlnAsp: 1.238 ± 0.026
1.176GlnGlu: 1.176 ± 0.037
1.02GlnPhe: 1.02 ± 0.029
2.224GlnGly: 2.224 ± 0.043
0.566GlnHis: 0.566 ± 0.019
1.602GlnIle: 1.602 ± 0.035
0.791GlnLys: 0.791 ± 0.028
2.739GlnLeu: 2.739 ± 0.046
0.746GlnMet: 0.746 ± 0.024
0.705GlnAsn: 0.705 ± 0.027
1.764GlnPro: 1.764 ± 0.037
1.087GlnGln: 1.087 ± 0.042
2.428GlnArg: 2.428 ± 0.042
1.529GlnSer: 1.529 ± 0.037
1.431GlnThr: 1.431 ± 0.035
2.21GlnVal: 2.21 ± 0.045
0.461GlnTrp: 0.461 ± 0.019
0.616GlnTyr: 0.616 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
10.401ArgAla: 10.401 ± 0.113
0.533ArgCys: 0.533 ± 0.02
4.591ArgAsp: 4.591 ± 0.061
4.236ArgGlu: 4.236 ± 0.068
3.17ArgPhe: 3.17 ± 0.055
5.706ArgGly: 5.706 ± 0.073
1.82ArgHis: 1.82 ± 0.039
4.272ArgIle: 4.272 ± 0.059
1.941ArgLys: 1.941 ± 0.039
8.487ArgLeu: 8.487 ± 0.103
2.004ArgMet: 2.004 ± 0.037
1.828ArgAsn: 1.828 ± 0.038
4.006ArgPro: 4.006 ± 0.065
2.415ArgGln: 2.415 ± 0.047
6.614ArgArg: 6.614 ± 0.079
3.593ArgSer: 3.593 ± 0.055
3.676ArgThr: 3.676 ± 0.049
5.284ArgVal: 5.284 ± 0.06
1.326ArgTrp: 1.326 ± 0.033
2.001ArgTyr: 2.001 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
6.266SerAla: 6.266 ± 0.075
0.368SerCys: 0.368 ± 0.017
2.872SerAsp: 2.872 ± 0.051
2.171SerGlu: 2.171 ± 0.039
2.029SerPhe: 2.029 ± 0.039
5.268SerGly: 5.268 ± 0.069
0.943SerHis: 0.943 ± 0.029
2.664SerIle: 2.664 ± 0.038
1.16SerLys: 1.16 ± 0.03
4.818SerLeu: 4.818 ± 0.063
1.038SerMet: 1.038 ± 0.027
1.276SerAsn: 1.276 ± 0.035
2.861SerPro: 2.861 ± 0.051
1.316SerGln: 1.316 ± 0.036
3.39SerArg: 3.39 ± 0.05
2.547SerSer: 2.547 ± 0.054
2.52SerThr: 2.52 ± 0.049
3.485SerVal: 3.485 ± 0.05
0.713SerTrp: 0.713 ± 0.026
1.43SerTyr: 1.43 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
6.501ThrAla: 6.501 ± 0.074
0.39ThrCys: 0.39 ± 0.017
2.818ThrAsp: 2.818 ± 0.043
2.123ThrGlu: 2.123 ± 0.043
1.864ThrPhe: 1.864 ± 0.042
5.471ThrGly: 5.471 ± 0.073
0.958ThrHis: 0.958 ± 0.028
3.12ThrIle: 3.12 ± 0.048
1.137ThrLys: 1.137 ± 0.032
5.827ThrLeu: 5.827 ± 0.071
1.123ThrMet: 1.123 ± 0.031
1.277ThrAsn: 1.277 ± 0.041
3.558ThrPro: 3.558 ± 0.054
1.414ThrGln: 1.414 ± 0.037
3.761ThrArg: 3.761 ± 0.058
2.627ThrSer: 2.627 ± 0.054
2.733ThrThr: 2.733 ± 0.057
4.254ThrVal: 4.254 ± 0.055
0.698ThrTrp: 0.698 ± 0.025
1.176ThrTyr: 1.176 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
10.585ValAla: 10.585 ± 0.097
0.542ValCys: 0.542 ± 0.02
4.442ValAsp: 4.442 ± 0.057
4.302ValGlu: 4.302 ± 0.05
2.215ValPhe: 2.215 ± 0.042
5.66ValGly: 5.66 ± 0.069
1.358ValHis: 1.358 ± 0.032
3.693ValIle: 3.693 ± 0.047
1.959ValLys: 1.959 ± 0.043
6.471ValLeu: 6.471 ± 0.071
1.664ValMet: 1.664 ± 0.034
2.014ValAsn: 2.014 ± 0.043
4.166ValPro: 4.166 ± 0.056
1.871ValGln: 1.871 ± 0.041
5.384ValArg: 5.384 ± 0.063
4.129ValSer: 4.129 ± 0.059
4.813ValThr: 4.813 ± 0.06
5.565ValVal: 5.565 ± 0.059
0.834ValTrp: 0.834 ± 0.027
1.364ValTyr: 1.364 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.435TrpAla: 1.435 ± 0.036
0.134TrpCys: 0.134 ± 0.011
0.684TrpAsp: 0.684 ± 0.022
0.583TrpGlu: 0.583 ± 0.018
0.609TrpPhe: 0.609 ± 0.022
0.994TrpGly: 0.994 ± 0.029
0.377TrpHis: 0.377 ± 0.016
0.693TrpIle: 0.693 ± 0.02
0.427TrpLys: 0.427 ± 0.018
1.803TrpLeu: 1.803 ± 0.04
0.327TrpMet: 0.327 ± 0.015
0.458TrpAsn: 0.458 ± 0.018
0.718TrpPro: 0.718 ± 0.023
0.562TrpGln: 0.562 ± 0.022
1.503TrpArg: 1.503 ± 0.041
0.919TrpSer: 0.919 ± 0.029
0.832TrpThr: 0.832 ± 0.024
0.839TrpVal: 0.839 ± 0.026
0.249TrpTrp: 0.249 ± 0.013
0.295TrpTyr: 0.295 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.862TyrAla: 2.862 ± 0.047
0.211TyrCys: 0.211 ± 0.013
1.634TyrAsp: 1.634 ± 0.041
1.109TyrGlu: 1.109 ± 0.03
0.881TyrPhe: 0.881 ± 0.026
2.228TyrGly: 2.228 ± 0.041
0.517TyrHis: 0.517 ± 0.016
0.885TyrIle: 0.885 ± 0.026
0.546TyrLys: 0.546 ± 0.023
2.155TyrLeu: 2.155 ± 0.035
0.418TyrMet: 0.418 ± 0.016
0.655TyrAsn: 0.655 ± 0.025
1.094TyrPro: 1.094 ± 0.029
0.703TyrGln: 0.703 ± 0.022
1.957TyrArg: 1.957 ± 0.035
1.186TyrSer: 1.186 ± 0.031
1.071TyrThr: 1.071 ± 0.038
1.652TyrVal: 1.652 ± 0.042
0.349TyrTrp: 0.349 ± 0.016
0.687TyrTyr: 0.687 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4226 proteins (1378064 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski